Search CTL/CD8+ T-Cell Epitope Database

Found 8 matching records:

Displaying record number 57704

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HXB2 Location	Gag(93-101) p17(93-101) DNA(1066..1092)	Gag Epitope Map
Author Location	Gag
Epitope	`EIKDTKEAL`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B*08)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	supervised treatment interruptions (STI), escape

Notes

Chronically infected HIV-1-infected patients were examined during treatment interruption (TI) for associations between viral control and presence of escape mutations. Patients 311 and 313 who had best viral control exhibited CTL response against at least 1-2 epitopes early in TI, followed by increasing numbers of epitopes recognized and increasing mutations developed in time . In patient 410 with worst viral load control only 2 epitopes elicited CTL response through all of TI, and no mutations were detected.
Epitope EIKDTKEAL elicited CTL response only at week 48 during TI in Patient 311.

References

Schweighardt2010 Becky Schweighardt, Terri Wrin, Duncan A. Meiklejohn, Gerald Spotts, Christos J. Petropoulos, Douglas F. Nixon, and Frederick M. Hecht. Immune Escape Mutations Detected within HIV-1 Epitopes Associated with Viral Control During Treatment Interruption. J. Acquir. Immune Defic. Syndr., 53(1):36-46, Jan 2010. PubMed ID: 19910798. Show all entries for this paper.

Displaying record number 58530

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HXB2 Location	Gag(93-101) p17(93-101) DNA(1066..1092)	Gag Epitope Map
Author Location	p17(93-101)
Epitope	`EIKDTKEAL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B08, B60)
Immunogen	HIV-1 infection
Country	Canada
Experimental methods	Other
Keywords	characterizing CD8+ T cells, elite controllers

Notes

Dual color EliSpot testing for IL-2 and IFN-γ was used to determine whether CTL restricted by MHC Class I alleles associated with slow progression to disease, viz. B*57 and B*27, had a more poly-functional profile than other CD8+ cells in a treatment-naive cohort from Montreal. B*57/B*27-restricted CTL producing IL-2 or IFN-γ or IL-2/IFN-γ, were similar in both slow progressors (SP) and progressors. Other non-protective MHC-I-restricted CTL producing both IL-2/IFN-γ, were greater in number in SP than progressors.
Within SP, peptides restricted by both protective and non-protective MHC-I alleles elicited CTL with the same functional profile. For progressors, peptides and epitopes restricted by protective B*57/B*27 alleles stimulated a higher proportion of dual-(IL-2/IFN-γ)secreting CTL.
HLA-B*08/B*60-restricted p17 optimal epitope EIKDTKEAL was tested in a dual color EliSpot and was found to elicit CTL that could produce IL-2 and/or IFN-γ.

References

Peretz2011 Yoav Peretz, Olivia Marra, Réjean Thomas, Danielle Legault, Pierre Côté, Mohamed-Rachid Boulassel, Danielle Rouleau, Jean-Pierre Routy, Rafick-Pierre Sékaly, Christos M Tsoukas, Cécile Tremblay, and Nicole F. Bernard. Relative Contribution of HIV-Specific Functional Lymphocyte Subsets Restricted by Protective and Non-Protective HLA Alleles. Viral Immunol, 24(3):189-198, Jun 2011. PubMed ID: 21668360. Show all entries for this paper.

Displaying record number 61995

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HXB2 Location	Gag(93-101) p17(93-101) DNA(1066..1092)	Gag Epitope Map
Author Location	Gag(93-101)
Epitope	`EIKDTKEAL`	Epitope Alignment
Species (MHC/HLA)	human(B*08:01)
Immunogen	peptide-HLA interaction
Experimental methods	Intracellular cytokine staining, Other
Keywords	assay or method development, NK cells

Notes

Contamination of what were previously thought to be highly specific epitopes identified through HLA IP-based immunopeptidomics is exposed. A low-level of co-isolation of erroneously assigned Class I-restricted peptides is found viz. HLA class II peptides, extended peptides from HIV Gag, and peptides from the B lymphoblastoid cell line 721.221 previously thought to be Class I deficient but which actually does present HLA-Cw*0102.
9/64 C8166 cell lysate identified epitopes are actually known epitopes. EIKDTKEAL is one of them, restricted by B*0801.

References

Partridge2018 Thomas Partridge, Annalisa Nicastri, Anna E. Kliszczak, Louis-Marie Yindom, Benedikt M. Kessler, Nicola Ternette, and Persephone Borrow. Discrimination Between Human Leukocyte Antigen Class I-Bound and Co-Purified HIV-Derived Peptides in Immunopeptidomics Workflows. Front. Immunol., 9:912, 2018. PubMed ID: 29780384. Show all entries for this paper.

Displaying record number 119

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HXB2 Location	Gag(93-101) p17(93-101) DNA(1066..1092)	Gag Epitope Map
Author Location	p17(93-101 LAI)
Epitope	`EIKDTKEAL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B60, B8)
Immunogen
Experimental methods
Keywords

Notes

Pers. comm. from A. Trocha and S. Kalams to C. Brander and B. Walker.

References

Brander1997 Christian Brander and Bruce Walker. Systematic Identification of Optimal HIV-1 CTL Epitopes. In Systematic Identification of Optimal HIV-1 CTL Epitopes. pages IV--1--IV--11. Los Alamos National Laboratory, Theoretical Biology and Biophysics, Los Alamos, New Mexico, 1997. URL: http://hiv-web.lanl.gov/immunology. Show all entries for this paper.

Displaying record number 55562

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HXB2 Location	Gag(93-101) p17(93-101) DNA(1066..1092)	Gag Epitope Map
Author Location	p17(93-101)
Epitope	`EIKDTKEAL`	Epitope Alignment
Epitope Name	EL9
Subtype	B
Species (MHC/HLA)	human(B8)
Immunogen	HIV-1 infection
Patient MHC/HLA	A1, A68:01, B51, B8, C15, Cw7, DQ2, DQ3, DR3, DR4
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	rate of progression, escape, mutation acquisition

Notes

An LTNP was followed longitudinally over 6 years and 3 sampling time points, until transition to disease. His HLA were heterozygous and not associated with nonprogressive infection, but 3 alleles were associated with poor clinical prognosis. No attenuating mutations in initial samplings, but an insertion in the V2 loop and continuing CCR5 coreceptor use were found.
A decrease in IFN-gamma response was seen over time, suggesting CTL dysfunction. An increase in T-cell epitopes targeted (e.g. emergence of YL8, YLKDQQLL and WY20, WKFDSRLAFHHMARELHPEY variants and gain of response to EL9, EIKDTKEAL, NL10, NSNPDCKTIL, AK9, AIFQSSMTK, WM8, WSMVRERM, VT15, WVHHTQGYFPDWQNYT) was seen concomitant with disease progression, reflecting viral replication and/or variants.
HLA-B8-restricted autologous epitope EIKDTKEAL was able to elicit CTL response only by the last time point. HLA restriction was predicted in this study based on the patient's HLA alleles and previous publication.

References

Kemal2008 Kimdar Sherefa Kemal, Tara Beattie, Tao Dong, Barbara Weiser, Rupert Kaul, Carla Kuiken, Julian Sutton, Dorothy Lang, Hongbing Yang, Yan Chun Peng, Ronald Collman, Sean Philpott, Sarah Rowland-Jones, and Harold Burger. Transition from Long-Term Nonprogression to HIV-1 Disease Associated with Escape from Cellular Immune Control. J. Acquir. Immune Defic. Syndr., 48(2):119-126, 1 Jun 2008. PubMed ID: 18520675. Show all entries for this paper.

Displaying record number 118

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HXB2 Location	Gag(93-101) p17(93-101) DNA(1066..1092)	Gag Epitope Map
Author Location	p17(93-101)
Epitope	`EIKDTKEAL`	Epitope Alignment
Species (MHC/HLA)	human(B8)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

A study of p17 variation considering known p17 epitopes and individuals with known HLA types revealed that p17 evolution is influenced by immune pressure from CTLs.

References

Birk1998 M. Birk, A. Vahlne, A. Sonnerborg, and M. Sallberg. Nonsynonymous Mutations within the Human Immunodeficiency Virus Type 1 p17 Gene Are Clustered to Sequences Binding to the Host Human Leukocyte Antigen Class I Molecules. AIDS Res. Hum. Retroviruses, 14:241-248, 1998. PubMed ID: 9491914. Show all entries for this paper.

Displaying record number 55237

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HXB2 Location	Gag(93-101) p17(93-101) DNA(1066..1092)	Gag Epitope Map
Author Location	p17(93-101 B1 and B2)
Epitope	`EIKDTKEAL`	Epitope Alignment
		Show epitope variants
Subtype	B, CRF01_AE
Species (MHC/HLA)	human(B8)
Immunogen	HIV-1 infection
Patient MHC/HLA	A3, A32, B62, B8, Cw3
Country	Netherlands
Experimental methods	Other
Keywords	subtype comparisons, computational epitope prediction, superinfection

Notes

The influence of superinfection upon changes in HIV-1 strains was studied in a triple infected subject. While continuous expression of all three strains was observed, the LTR promoters of subtype AE had highest activity of all 3 strains, and subtype B2 had the lowest. Env-V3 sequences were present in higher numbers in strains B2 and CRF01_AE. Recombination was seen between viruses B1/B2 in gag and vpr genes.
This HLA-B08 restricted epitope, EIKDTKEAL, varied to EvKDTKEAL in 83% of viruses in B1 within 4 years, dvKDTKEAL at the earliest time point taken in B2 with no changes over time, and EIlDTKEAL in AE at the earliest time point taken, with no changes over time. A reversion was seen in B1 to dIKDTKEAL i.e. v to I, suggesting a lack of CTL pressure on this sequence.

References

Kozaczynska2007 Karolina Kozaczynska, Marion Cornelissen, Peter Reiss, Fokla Zorgdrager, and Antoinette C. van der Kuyl. HIV-1 Sequence Evolution In Vivo after Superinfection with Three Viral Strains. Retrovirology, 4:59, 2007. PubMed ID: 17716368. Show all entries for this paper.

Displaying record number 117

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HXB2 Location	Gag(93-101) p17(93-101) DNA(1066..1092)	Gag Epitope Map
Author Location	p17(93-101)
Epitope	`EIKDTKEAL`	Epitope Alignment
Species (MHC/HLA)	human(B8)
Immunogen	peptide-HLA interaction
Experimental methods
Keywords

Notes

Examined in the context of motifs important for HLA-B8 binding, predicted epitope based on Achour et al.

References

DiBrino1994a M. DiBrino, K. C. Parker, J. Shiloach, R. V. Turner, T. Tsuchida, M. Garfield, W. E. Biddison, and J. E. Coligan. Endogenous Peptides with Distinct Amino Acid Anchor Residue Motifs Bind to HLA-A1 and HLA-B8. J. Immunol., 152:620-631, 1994. PubMed ID: 7506728. Show all entries for this paper.