Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 58686

HXB2 Location	Rev(10-18) DNA(5997..6023)	Rev Epitope Map
Author Location	Rev
Epitope	EELLKTVRL	Epitope Alignment
		Show epitope variants
Epitope Name	EL9
Subtype	B
Species (MHC/HLA)	human(B*4402, B*4403)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*0301, A*2902, B*0702, B*4403, C*07
Country	Belgium
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	escape, optimal epitope

Notes

• Rev epitope EELLKTVRL (EL9) is immunogenic despite its resemblance to human self-antigen nucleolar protein 6 (NOL6). A case study following one subject infected with HIV subtype B and on ART was used to show that upon treatment interruption while viral load (VL) and CD4+ counts remained stable, EL9 mutated. The EL9 mutations did not impair Rev function but did decrease or abolish CTL recognition.
• EELLKTVRL (EL9) was shown to be the optimal epitope and HLA-B*4403 the restricting molecule. B*4402 is also able to present EL9, but results in lower cytokine secretion. B*4404 cannot present EL9.

References

Allard2012 S. D. Allard, A. L. de Goede, B. De Keersmaecker, C. Heirman, P. Lacor, A. D. M. E. Osterhaus, C. Demanet, K. Thielemans, R. A. Gruters, and J. L. Aerts. Sequence Evolution and Escape from Specific Immune Pressure of an HIV-1 Rev Epitope with Extensive Sequence Similarity to Human Nucleolar Protein 6. Tissue Antigens, 79(3):174-185, Mar 2012. PubMed ID: 22309258. Show all entries for this paper.

Displaying record number 61765

HXB2 Location	Rev(10-18) DNA(5997..6023)	Rev Epitope Map
Author Location	Rev(10-18)
Epitope	EELLKTVRL	Epitope Alignment
Epitope Name	EL9
Species (MHC/HLA)	human(B*4402, B*4403)
Immunogen
Experimental methods
Keywords	optimal epitope

Notes

• C. Brander notes this as a B*4402/4403-restricted Rev epitope (Allard2016).

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 54980

HXB2 Location	Rev(10-18) DNA(5997..6023)	Rev Epitope Map
Author Location	Rev(10-18)
Epitope	EELLKTVRL	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection, peptide-HLA interaction
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	immunodominance, T-cell crossreactivity to human proteins

Notes

• Resemblance between viral and host peptides may preclude immune reactivity against such epitopes. This was shown using ELISpot to test recognition against the entire viral genome in 314 HIV-infected subjects. Also, 944 overlapping Nef variants were tested for 30 patients. This inverse relationship between similarity of HIV epitope to host proteome and its immunogenicity has implications in vaccine design.
• Other factors, however, like entropy effects and forbidden residue usage or even hydrophobicity or disorder prediction scores were more influential than this similarity to self, with regards to immunogenicity of HIV-1. Also, forbidden aa ratios at the C-terminus of CTL epitopes and similarity to host proteins are positively correlated. Low disorder peptides elicit broader CTL responses and are better recognized.
• This HIV-1 epitope, EELLKTVRL, is similar to human protein nucleolar RNA associated protein, sequence EELLKeVRL, and a human protein, sequence LLKTVRLlRLL.

References

Rolland2007a Morgane Rolland, David C. Nickle, Wenjie Deng, Nicole Frahm, Christian Brander, Gerald H. Learn, David Heckerman, Nebosja Jojic, Vladimir Jojic, Bruce D. Walker, and James I. Mullins. Recognition of HIV-1 Peptides by Host CTL Is Related to HIV-1 Similarity to Human Proteins. PLoS ONE, 2(9):e823, 2007. PubMed ID: 17786195. Show all entries for this paper.