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Displaying record number 55666

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HXB2 Location	Gag(295-304) p24(163-172) DNA(1672..1701)	Gag Epitope Map
Author Location	Gag
Epitope	DYVDRFFKTL	Epitope Alignment
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 infection, HIV-1 or HIV-2 infection
Country	Belgium, Senegal
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	subtype comparisons, variant cross-reactivity, HIV-2

Notes

• To compare HIV-1 and HIV-2 CTL responses to Gag as far as homologous levels of response and cross-reactivity, 12 consecutive Gag OLP pools were used with cells from 17 HIV-1 and 17 HIV-2 patients in enhanced IFN-gamma ELISpot assays. Gag-specific homologous CTL responses were significantly higher in HIV-2 patients, but cross-reactivity in HIV-1-infected patients was broader and stronger.
• Cross-reactivity correlated with sequence similarity in HIV-2 patients, but not HIV-1 patients. CD4+ T-cell counts of HIV-2-infected patients correlated directly with homologous responses and inversely with cross-reactive responses; this was not true of HIV-1-infected subjects.
• The authors favor a model in which high HIV-2-specific CTL responses control its replication, containing immune evasion and thus limiting the possibility of cross-reaction to HIV-1 homologous epitopes.
• HIV-1 Gag epitope DYVDRFFKTL is probably cross-recognized with its homologous HIV-2 epitope, SYVDRFYKSL.

References

Jennes2008 Wim Jennes, Makhtar Camara, Tandakha Dièye, Souleymane Mboup, and Luc Kestens. Higher Homologous and Lower Cross-Reactive Gag-Specific T-Cell Responses in Human Immunodeficiency Virus Type 2 (HIV-2) Than in HIV-1 Infection. J. Virol., 82(17):8619-8628, Sep 2008. PubMed ID: 18562522. Show all entries for this paper.

Displaying record number 59431

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HXB2 Location	Gag(295-304) p24(163-172) DNA(1672..1701)	Gag Epitope Map
Author Location	Gag(295-304)
Epitope	DYVDRFFKTL	Epitope Alignment
		Show epitope variants
Epitope Name	Gag295-304
Species (MHC/HLA)	human(C*03:04)
Immunogen	in vitro stimulation or selection
Experimental methods	Degranulation, CD107a and b cell surface mobilization, Other, T2 stabilization assay
Keywords	computational epitope prediction, NK cells

Notes

• HIV may evade killer T cells through the selection of variants that interfere with the interactions of inhibitory killer cell Ig-like receptors (KIRs). This study identified Cw*0304-restricted epitopes that alter KIR2DL2 binding. Seven peptides were selected that were able to bind to C*0304. Three of the seven were previously described.
• DYVDRFFKTL was identified based on its binding to Cw*0304 in vitro. It had been previously identified as a Cw*0304 epitope (YVDRFFKTL and YVDRFFKVL). Gag295-304 enables binding of KIR2DL2 to HLA-Cw*0304 and results in inhibition of KIR2DL2+ primary NK cells.
• P9 variants of this epitope (especially T303V variant) in HLA-Cw*03+ subjects also binds the inhibitory NK cell receptor, KIR2DL2, evading NK-cell mediated killing.

References

vanTeijlingen2014 Nienke H. van Teijlingen, Angelique Hölzemer, Christian Körner, Wilfredo F. García-Beltrán, Jamie L. Schafer, Lena Fadda, Todd J. Suscovich, Christian Brander, Mary Carrington, David T Evans, Debbie van Baarle, and Marcus Altfeld. Sequence Variations in HIV-1 p24 Gag-Derived Epitopes Can Alter Binding of KIR2DL2 to HLA-C*03:04 and Modulate Primary Natural Killer Cell Function. AIDS, 28(10):1399-1408, 19 Jun 2014. PubMed ID: 24785948. Show all entries for this paper.