Search CTL/CD8+ T-Cell Epitope Database

Found 7 matching records:

Displaying record number 53572

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HXB2 Location	Pol(86-94) Protease(30-38) DNA(2340..2366)	Pol Epitope Map
Author Location	Pol(87-95)
Epitope	`DTVLEEMNL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A28)
Immunogen	HIV-1 infection
Patient MHC/HLA	A28, A29, B14, B44, Cw8
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords

Notes

Two-thirds of all mutations arising in non-envelope proteins during the transition from acute to chronic infection were found to be due to either CD8 T-cell-associated selective pressures or reversion of HLA-I-associated mutations selected in a previous host.The predominance of escape mutations at highly polymorphic sites was observed across different HIV-1 clades.
This epitope was reactive, but escape mutations did not accrue in it over time.

References

Allen2005a Todd M. Allen, Marcus Altfeld, Shaun C. Geer, Elizabeth T. Kalife, Corey Moore, Kristin M. O'Sullivan, Ivna DeSouza, Margaret E. Feeney, Robert L. Eldridge, Erica L. Maier, Daniel E. Kaufmann, Matthew P. Lahaie, Laura Reyor, Giancarlo Tanzi, Mary N. Johnston, Christian Brander, Rika Draenert, Jurgen K. Rockstroh, Heiko Jessen, Eric S. Rosenberg, Simon A. Mallal, and Bruce D. Walker. Selective Escape from CD8+ T-Cell Responses Represents a Major Driving Force of Human Immunodeficiency Virus Type 1 (HIV-1) Sequence Diversity and Reveals Constraints on HIV-1 Evolution. J. Virol., 79(21):13239-13249, Nov 2005. PubMed ID: 16227247. Show all entries for this paper.

Displaying record number 53385

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HXB2 Location	Pol(86-94) Protease(30-38) DNA(2340..2366)	Pol Epitope Map
Author Location	( B consensus)
Epitope	`DTVLEEMNL`	Epitope Alignment
Epitope Name	DL9
Subtype	B
Species (MHC/HLA)	human(A68)
Immunogen	HIV-1 infection
Patient MHC/HLA	A31, A68, B7, B70, Cw1, Cw7
Country	United States
Experimental methods	Chromium-release assay, Cytokine production, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	assay or method development, memory cells, characterizing CD8+ T cells

Notes

Using a flow-cytometric cytotoxicity assay based on caspase-3 activation in dying target cells, it was shown that the subset of HIV-1-specific CD8+ T cells secreting both IFN-gamma and TNF-alpha exhibit stronger cytotoxic activity than those secreting only IFN-gamma. These cells also exhibited stronger intracellular perforin expression. No association between HIV-1-specific CD8+ T-cell maturation phenotypes and intracellular perforin expression was found.
1/9 individuals recognized this epitope.

References

Lichterfeld2004b Mathias Lichterfeld, Xu G. Yu, Michael T. Waring, Stanley K. Mui, Mary N. Johnston, Daniel Cohen, Marylyn M. Addo, John Zaunders, Galit Alter, Eunice Pae, Daryld Strick, Todd M. Allen, Eric S. Rosenberg, Bruce D. Walker, and Marcus Altfeld. HIV-1-Specific Cytotoxicity Is Preferentially Mediated by a Subset of CD8+ T Cells Producing both Interferon-gamma and Tumor Necrosis Factor-alpha. Blood, 104(2):487-394, 15 Jul 2004. PubMed ID: 15059848. Show all entries for this paper.

Displaying record number 55029

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HXB2 Location	Pol(86-94) Protease(30-38) DNA(2340..2366)	Pol Epitope Map
Author Location
Epitope	`DTVLEEMNL`	Epitope Alignment
Species (MHC/HLA)	human(A68)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding
Keywords	binding affinity, immunodominance, optimal epitope

Notes

CTL responses were tested for both HIV-1 and EBV derived epitopes restricted by HLA-A, -B and -C alleles. HLA-B alleles more frequently induce detectable responses and that the responses are generally of greater magnitude than those for HLA-A and -C alleles.
The magnitude and frequency of CTL recognition were related to each other and that TCR avidity for HLA/peptide complexes is a more important determinant for magnitude of response than peptide binding affinity to HLA.
EBV response patterns were not significantly altered by HIV coinfection.
Epitope DTVLEEMNL elicited a magnitude of response of 565 SFC with a functional avidity of 0.5nM and binding affinity of >40,000nM.

References

Bihl2006 Florian Bihl, Nicole Frahm, Loriana Di Giammarino, John Sidney, Mina John, Karina Yusim, Tonia Woodberry, Kaori Sango, Hannah S. Hewitt, Leah Henry, Caitlyn H. Linde, John V. Chisholm, III, Tauheed M. Zaman, Eunice Pae, Simon Mallal, Bruce D Walker, Alessandro Sette, Bette T. Korber, David Heckerman, and Christian Brander. Impact of HLA-B Alleles, Epitope Binding Affinity, Functional Avidity, and Viral Coinfection on the Immunodominance of Virus-Specific CTL Responses. J. Immunol., 176(7):4094-4101, 1 Apr 2006. PubMed ID: 16547245. Show all entries for this paper.

Displaying record number 398

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HXB2 Location	Pol(86-94) Protease(30-38) DNA(2340..2366)	Pol Epitope Map
Author Location	Pol( subtype B)
Epitope	`DTVLEEMNL`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(A*68:02)
Immunogen	HIV-1 exposed seronegative
Experimental methods
Keywords	subtype comparisons

Notes

HIV-specific CTL were found in exposed seronegative prostitutes from Nairobi---these CTL may confer protection.
Seroprevalence in this cohort is 90-95% and their HIV-1 exposure is among the highest in the world.
Most isolated HIV strains are clade A in Nairobi, although clades C and D are also found -- B clade epitopes are often cross-reactive, however stronger responses are frequently observed using A or D clade versions of epitopes.
This epitope is conserved among B and D clade viruses.
The Clade A version of the epitope: DTVLEDINL.
This epitope was recognized by two different exposed and uninfected prostitutes.
This epitope was identified by screening 49 HIV-1 peptides with the predicted A*6802 anchor residue motif x(VT)xxxxxx(VL)
Database note: First author "Rowland-Jones" is also found as "RowlandJones" on annotated papers in this database.

References

Rowland-Jones1998 S. L. Rowland-Jones, T. Dong, K. R. Fowke, J. Kimani, P. Krausa, H. Newell, T. Blanchard, K. Ariyoshi, J. Oyugi, E. Ngugi, J. Bwayo, K. S. MacDonald, A. J. McMichael, and F. A. Plummer. Cytotoxic T cell responses to multiple conserved HIV epitopes in HIV-resistant prostitutes in Nairobi. J. Clin. Invest., 102(9):1758-65, 1 Nov 1998. PubMed ID: 9802890. Show all entries for this paper.

Displaying record number 57331

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HXB2 Location	Pol(86-94) Protease(30-38) DNA(2340..2366)	Pol Epitope Map
Author Location	Pol
Epitope	`DTVLEEMNL`	Epitope Alignment
Subtype	A, CRF02_AG, CRF06_cpx, CRF09_cpx
Species (MHC/HLA)	human(A*68:02)
Immunogen	HIV-1 infection
Country	Burkina Faso
Experimental methods	Other
Keywords	drug resistance

Notes

Drug-associated mutations were analyzed in HIV-1 pol gene from drug-naive patients in Burkina Faso. 12.5% of all mutations were drug resistance-associated, suggesting either transmission from an ART-treated tramsitter or prior undocumented use of ART by the subject. Subtype specific polymorphisms were also seen, some of them within CTL epitopes.
B-consensus HLA-A*6802-restricted epitope DTVLEEMNL was found as variant DTVLEEiNL in Burkina Faso virus subtype CRF02.AG and CRF09.cpx sequences. Variant DTVLEdiNL was seen in subtypes CRF06.cpx and A. 5% Burkinabe carry HLA-A*6802.

References

Tebit2009 Denis M. Tebit, Lassana Sangaré, Fabrice Tiba, Yameogo Saydou, Aline Makamtse, Hermann Somlare, Guillaume Bado, Boris G. Kouldiaty, Inoussa Zabsonre, Sibiri L. Yameogo, Kanokporn Sathiandee, Joseph Y. Drabo, and Hans-Georg Kräusslich. Analysis of the Diversity of the HIV-1 pol Gene and Drug Resistance Associated Changes among Drug-Naïve Patients in Burkina Faso. J. Med. Virol., 81(10):1691-701, Oct 2009. PubMed ID: 19697403. Show all entries for this paper.

Displaying record number 59576

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HXB2 Location	Pol(86-94) Protease(30-38) DNA(2340..2366)	Pol Epitope Map
Author Location	Pol(86-94)
Epitope	`DTVLEEMNL`	Epitope Alignment
Epitope Name	DL9
Species (MHC/HLA)	human(A*68:02)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
DTVLEEMNL had a significantly positive association with HLA A*6802; it was recognized by 2/26 A*6802-positive subjects and 0/315 A*6802-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 61261

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HXB2 Location	Pol(86-94) Protease(30-38) DNA(2340..2366)	Pol Epitope Map
Author Location	Protease
Epitope	`DTVLEEMNL`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Mexico, Spain
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	drug resistance

Notes

Pol (PR-RT) CTL epitopes containing drug resistance (DR) mutations, but capable of eliciting immune response were found in ART-naive subjects. Most CTL response detected was to either a DR-containing or wild type (WT) peptide but not both. A majority of the 22 most-recognized peptides of the 218 tested were DR-containing. Capability of immune recognition and response to DR peptides was usually not associated with the presence of DR mutations in the infecting virus, but this association did exist for WT peptides.
WT PRotease peptide DTVLEEMNL was recognized by 5/49 patients tested. It overlaps a DR position, L33I, but those 5 subjects did not respond to the DR peptide. 10 different subjects however, did recognize DR peptide DTViEEMNL.

References

Blanco-Heredia2016 Juan Blanco-Heredia, Aarón Lecanda, Humberto Valenzuela-Ponce, Christian Brander, Santiago Ávila-Rios, and Gustavo Reyes-Terán. Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naive HIV-Infected Individuals. PLoS One, 11(1):e0147571, 2016. PubMed ID: 26808823. Show all entries for this paper.