Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 1690

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HXB2 Location	Gag(203-212) p24(71-80) DNA(1396..1425)	Gag Epitope Map
Author Location	p24(203-212)
Epitope	`DTINEEAAEW`	Epitope Alignment
Species (MHC/HLA)	human(B53)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B53 women, 0/2 HEPS and 7/9 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in 4 of the 7/9 responsive HIV-1 infected women.

References

Kaul2001a R. Kaul, T. Dong, F. A. Plummer, J. Kimani, T. Rostron, P. Kiama, E. Njagi, E. Irungu, B. Farah, J. Oyugi, R. Chakraborty, K. S. MacDonald, J. J. Bwayo, A. McMichael, and S. L. Rowland-Jones. CD8(+) lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J. Clin. Invest., 107(10):1303-10, May 2001. URL: http://www.jci.org/cgi/content/full/107/10/1303. PubMed ID: 11375420. Show all entries for this paper.

Displaying record number 1713

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HXB2 Location	Gag(203-212) p24(71-80) DNA(1396..1425)	Gag Epitope Map
Author Location	p24(203-212 subtype A consensus)
Epitope	`DTINEEAAEW`	Epitope Alignment
		Show epitope variants
Subtype	A
Species (MHC/HLA)	human(B53)
Immunogen	HIV-1 infection
Country	Gambia
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, HLA binding
Keywords	binding affinity, subtype comparisons, epitope processing, variant cross-reactivity, optimal epitope, novel epitope

Notes

In clade A infected Gambians, three HLA-B53 epitopes were defined in Gag p24 using ELISPOT, tetramer, and cytotoxicity assays.
Two of the new epitopes lacked the predicted P2 anchors, DTINEEAAEW and QATQEVKNM, and bound to B53 with high affinity, thus extending the anchor residue motif for B53 and the related B35.
Two overlapping 20 mer peptides carry this complete epitope, but only one stimulates recognition, which could be due to different peptide processing.
DTINEEAAEW was recognized in only 2/7 HLA-B53 subjects.
DTINEEAAEW was not A subtype specific and there was cross-recognition although diminished, of the subtype B, C, and D variant, ETINEEAAEW.
In one of the two subjects there was cross-recognition of the HIV-2 version of the epitope, EIINEEAADW.

References

Dorrell2001 L. Dorrell, B. E. Willcox, E. Y. Jones, G. Gillespie, H. Njai, S. Sabally, A. Jaye, K. DeGleria, T. Rostron, E. Lepin, A. McMichael, H. Whittle, and S. Rowland-Jones. Cytotoxic T lymphocytes recognize structurally diverse, clade-specific and cross-reactive peptides in human immunodeficiency virus type-1 gag through HLA-B53. Eur. J. Immunol., 31(6):1747-56, Jun 2001. PubMed ID: 11385619. Show all entries for this paper.

Displaying record number 2005

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HXB2 Location	Gag(203-212) p24(71-80) DNA(1396..1425)	Gag Epitope Map
Author Location	p24
Epitope	`DTINEEAAEW`	Epitope Alignment
Epitope Name	Gag-DW10; p24-DW10
Subtype	B
Species (MHC/HLA)	human(B*53:01)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	HAART, ART

Notes

Among HIV+ individuals who carried HLA B*5301, 2/15 (13%) recognized this epitope.

References

Sabbaj2003 Steffanie Sabbaj, Anju Bansal, G. Douglas Ritter, Christopher Perkins, Bradley H. Edwards, Ethan Gough, Jianming Tang, James J. Szinger, Bette Korber, Craig M. Wilson, Richard A. Kaslow, Mark J. Mulligan, and Paul A. Goepfert. Cross-Reactive CD8+ T Cell Epitopes Identified in US Adolescent Minorities. J. Acquir. Immune Defic. Syndr., 33(4):426-438, 1 Aug 2003. PubMed ID: 12869831. Show all entries for this paper.

Displaying record number 58828

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HXB2 Location	Gag(203-212) p24(71-80) DNA(1396..1425)	Gag Epitope Map
Author Location	p24
Epitope	`DTINEEAAEW`	Epitope Alignment
Epitope Name	DW10
Subtype	C
Species (MHC/HLA)	human(B*58:01)
Immunogen	HIV-1 infection
Country	Botswana, South Africa, Zimbabwe
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding, Intracellular cytokine staining, Tetramer binding
Keywords	optimal epitope, HLA associated polymorphism, novel epitope

Notes

1,010-HIV C clade-chronically infected subjects were tested in order to distinguish levels of immune control by related but different HLA-B*57/58. B*57 micropolymorphisms (B*5703 and B*5702) as well as B*5801 have differing CTL specificity and also present different p24 Gag epitopes. This results in different immune selection pressures on the HIV-C virus.
Though all three studied HLA alleles, B*5801, B*5702 and B*5703 are significantly associated with lower viral-load (VL) set points, HLA-B*5703, followed closely by B*5702 is the most potent controller with lowest VL and HLA-B*5702, followed by B*5703 and then B*5801 are associated with highest CD4 counts.
By using 410 overlapping 18-mers that span the HIV-C proteome in EliSpot assays, 17 epitopes were identified that are presented by one of HLAs-B*5801/5702/5703, 13 of them novel.
Novel epitope DW10 p24, DTINEEAAEW, was confirmed to be optimal by peptide truncation and restricted by HLA-B*5801 using CTL from subject N067. ˜50% B*5801-positive responders recognized DW10 p24, while <20% of B*5702- and B*5703-positive responders recognized it.
The binding affinity of DW10 is greatest to B*5702, but stability is greatest with HLA-B*5801.
No B*5702-positive subjects carried mutations in DW10 p24, while <10% of HLA-B*801 subjects and ∼10% HLA-B*5703 and non-B*57 subjects sustained DW10 variations.

References

Kloverpris2012 Henrik N. Kloverpris, Anette Stryhn, Mikkel Harndahl, Mary van der Stok, Rebecca P. Payne, Philippa C. Matthews, Fabian Chen, Lynn Riddell, Bruce D. Walker, Thumbi Ndung'u, Søren Buus, and Philip Goulder. HLA-B*57 Micropolymorphism Shapes HLA Allele-Specific Epitope Immunogenicity, Selection Pressure, and HIV Immune Control. J. Virol., 86(2):919-929, Jan 2012. PubMed ID: 22090105. Show all entries for this paper.

Displaying record number 53893

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HXB2 Location	Gag(203-212) p24(71-80) DNA(1396..1425)	Gag Epitope Map
Author Location	p24(71-80)
Epitope	`DTINEEAAEW`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	India
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, computational epitope prediction, immunodominance

Notes

PBMCs from HIV-1 infected Indian subjects were tested for HIV-1 Gag (with C clade peptides), Nef (with B clade peptides), and Env (clade B peptides) specific T-cell responses. 5 immunodominant regions conserved across clades were identified in Gag and Nef. 3 antigenic regions were found to be recognized by CTLs in the Indian subjects; these regions were not identified as immunodominant in studies from Africa.
PBMCs from HIV-1 infected Indian subjects were tested for HIV-1 Gag (with C clade peptides), Nef (with B clade peptides), and Env (clade unspecified) specific T-cell responses. 5 immunodominant regions conserved across clades were identified in Gag and Nef. 3 antigenic regions were found to be recognized by CTLs in the Indian subjects; these regions were not identified as immunodominant in studies from Africa.
26 epitopes were predicted within reactive peptides, of which 90% were clustered in the conserved immunodominant regions of Gag and Nef.
3 of the epitopes were highly conserved across clades, and 7 novel epitopes were found.
Epitope DTINEEAAEW is highly conserved across clades, >70% to clades A and C. It was predicted to have HLA-B*5801 restriction.

References

Thakar2005 Madhuri R. Thakar, Leena S. Bhonge, Samir K. Lakhashe, U. Shankarkumar, Suvarna S. Sane, Smita S. Kulkarni, Bharati A. Mahajan, and Ramesh S. Paranjape. Cytolytic T Lymphocytes (CTLs) from HIV-1 Subtype C-Infected Indian Patients Recognize CTL Epitopes From a Conserved Immunodominant Region of HIV-1 Gag and Nef. J. Infect. Dis., 192(5):749-759, 1 Sep 2005. PubMed ID: 16088824. Show all entries for this paper.