Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 53850

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HXB2 Location	Nef(186-194) DNA(9352..9378)	Nef Epitope Map
Author Location	Nef(186-194)
Epitope	DSRLAFHHM	Epitope Alignment
		Show epitope variants
Epitope Name	DM9
Subtype	B
Species (MHC/HLA)	human(A*24)
Immunogen	HIV-1 infection
Patient MHC/HLA	M-1003: A*24, A*30, B*39, B*47, C*12, C*17; P-1189: A*23, A*24, B*07, B*39, C*12, C*17; M-1001: A*03, A*31, B*08, B*15, C*04, C*07; P-1024: A*24, A*31, B*15, B*47, C*04, C*07
Country	United Kingdom
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	responses in children, mother-to-infant transmission, escape, acute/early infection, viral fitness and reversion

Notes

• CD8 T-cell responses were examined in mother-infant pairs. Escape variants were commonly detected in maternal plasma. Early infant plasma viruses showed heterogeneity of gag and nef gene sequences as well as mother-to-child transmission of CD8 T-cell escape variants. The stability of escape mutants in the infant over time was determined by infant HLA haplotype and viral fitness.
• Sequential plasma specimens from infants showed changes in CD8 T-cell epitope sequences, suggesting that infants are capable of generating virus-specific CD8 T-cell responses.
• DSRLAFHHM is a known A*24 epitope. A known escape variant, DSRLAFqHM, was transmitted from an A*24- mother (M-1001) to her A*24+ infant (P-1024), where it was gradually lost over time, present in 6/10 clones at 2 months, 7/10 at 4 months, and 0/10 at 15 months.
• Another escape variant was present in an A*24+ mother, DStLAFqHk and this form was transmitted to her A*24+ infant where it persisted in 30/30 sequences sampled over 12 months.
• DSRLAFHHM had higher responder cell frequencies in the A*24+ mother (M-1003) than DStLAFqHk. Her A*24+ infant (P-1189) did not recognize either form. The variant DSRLAFqHM also stimulated lower responder cell frequencies.

References

Sanchez-Merino2005 Victor Sanchez-Merino, Siwei Nie, and Katherine Luzuriaga. HIV-1-Specific CD8+ T Cell Responses and Viral Evolution in Women and Infants. J. Immunol., 175(10):6976-6986, 15 Nov 2005. PubMed ID: 16272358. Show all entries for this paper.

Displaying record number 1662

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HXB2 Location	Nef(186-194) DNA(9352..9378)	Nef Epitope Map
Author Location	Nef(186-194)
Epitope	DSRLAFHHM	Epitope Alignment
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

• ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Kaul2001a R. Kaul, T. Dong, F. A. Plummer, J. Kimani, T. Rostron, P. Kiama, E. Njagi, E. Irungu, B. Farah, J. Oyugi, R. Chakraborty, K. S. MacDonald, J. J. Bwayo, A. McMichael, and S. L. Rowland-Jones. CD8(+) lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J. Clin. Invest., 107(10):1303-10, May 2001. URL: http://www.jci.org/cgi/content/full/107/10/1303. PubMed ID: 11375420. Show all entries for this paper.

Displaying record number 53020

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HXB2 Location	Nef(186-194) DNA(9352..9378)	Nef Epitope Map
Author Location	Nef(186-194)
Epitope	DSRLAFHHM	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, CD8 T-cell Elispot granzyme B
Keywords	Th1, characterizing CD8+ T cells

Notes

• Only 20% of CD8+ T-cells produce IFN-gamma and granzyme B simultaneously (Tc1a). Two additional subpopulations of HIV specific CD8 cells are found, each one constituting 30-40% of the CD8 cell pool. One of these (Tc1b) secretes IFN-gamma only, and the other one (Tc1c) secretes GzB only.
• Two of seven patients responded to this peptide with GzB producing cells, while none of the patients responded with IFN-gamma producing cells.

References

Kleen2004 Thomas O. Kleen, Robert Asaad, Samuel J. Landry, Bernhard O. Boehm, and Magdalena Tary-Lehmann. Tc1 Effector Diversity Shows Dissociated Expression of Granzyme B and Interferon-gamma in HIV Infection. AIDS, 18(3):383-392, 20 Feb 2004. PubMed ID: 15090789. Show all entries for this paper.