Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 55885

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HXB2 Location	Gag(298-308) p24(166-176) DNA(1681..1713)	Gag Epitope Map
Author Location	Gag
Epitope	DRFYKTLRAEQ	Epitope Alignment
Subtype	A, B
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Country	Sweden
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	subtype comparisons

Notes

• By studying Gag p24 in different subtypes of subjects, previously discovered and new epitopes were identified. New T cell epitopes, both CD8 and CD4, related to the patients' HLA are described.
• T cells cross-reacted to peptides from different patient subtypes, and this cross-reactivity was distributed along the entire p24 protein.
• HLA-B14-restricted epitope DRFYKTLRAEQ is from subtype A and B peptide libraries, and is reactive as part of peptide YVDRFYKTLRAEQAS in a subtype B-carrying subject.

References

Gudmundsdotter2008 L. Gudmundsdotter, D. Bernasconi, B. Hejdeman, E. Sandstrom, A. Alaeus, K. Lidman, B. Ensoli, B. Wahren, and S. Buttò. Cross-Clade Immune Responses to Gag p24 in Patients Infected with Different HIV-1 Subtypes and Correlation with HLA Class I and II Alleles. Vaccine, 26(40):5182-5187, 19 Sep 2008. PubMed ID: 18479789. Show all entries for this paper.

Displaying record number 53222

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HXB2 Location	Gag(298-308) p24(166-176) DNA(1681..1713)	Gag Epitope Map
Author Location	Gag(295-305 BORI)
Epitope	DRFYKTLRAEQ	Epitope Alignment
Epitope Name	Gag DQ11
Subtype	B
Species (MHC/HLA)	human(B*14:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	BORI: A02, A29, B14, B44
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	dynamics, acute/early infection, autologous responses, optimal epitope

Notes

• Primary CD8+ T-cell response to Env, Tat and Gag and the extent, kinetics and mechanisms of viral escape were examined in three patients. Rapid escape, within weeks from infection, from HIV specific CTL responses was observed in all three patients, but the kinetics and extent of the escape differed depending on the breadth and co-dominant distribution of CTL-mediated pressure. The two patients that rapidly declined had more focused immunodominant responses, while the single patient that had low viral load and stable CD4 counts for seven years had a broad co-dominant response and less escape.
• The patient BORI had high viral loads and rapid CD4 decline. BORI mounted 8 detected responses. BORI did not control viral replication well, and escape mutations occurred early and in most epitopes, 6/7 that were precisely identified.
• DRFYKTLRAEQ didn't vary. There was no response in acute infection to this epitope, but the response was detectable by early infection.
• Patient BORI autologous epitope DRFYKTLRAEQ overlaps with patient SUMA autologous epitopes DYVDRFYKT & RAEQASQEV.
• Dynamics of this response are shown in Fig. S2 of Yang2019 which developed mathematical models with data from Jones2004. Yang2019 concluded that, in general, relative CTL response breadth increased moderately during the first symptomatic month and then remained relatively stable through the first 400 days post-infection. Analysis also suggested slow expansion kinetics for most HIV-specific CTL responses and the presence of intra- and interclonal competition.
• Yang2019 republished this sequence with a 2 AA truncation (DYVDRFYKT).

References

Jones2004 Nicola A. Jones, Xiping Wei, Darren R. Flower, MaiLee Wong, Franziska Michor, Michael S. Saag, Beatrice H. Hahn, Martin A. Nowak, George M. Shaw, and Persephone Borrow. Determinants of Human Immunodeficiency Virus Type 1 Escape from the Primary CD8+ Cytotoxic T Lymphocyte Response. J. Exp. Med., 200(10):1243-1256, 15 Nov 2004. PubMed ID: 15545352. Show all entries for this paper.

Yang2019 Yiding Yang, and Vitaly V. Ganusov. Defining Kinetic Properties of HIV-Specific CD8(+) T-Cell Responses in Acute Infection. Microorganisms, 7(3):microorganisms7030069 doi, Mar 2019. PubMed ID: 30836625 Show all entries for this paper.

Displaying record number 60927

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HXB2 Location	Gag(298-308) p24(166-176) DNA(1681..1713)	Gag Epitope Map
Author Location	p24(166-176)
Epitope	DRFYKTLRAEQ	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*14:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	94153: A*25:01, A*68:01, B*14:02, B*44:02, C*05:01, C*08:02
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	acute/early infection

Notes

• The extent to which set-point viremia is affected by early HIV-infection T cell responses to either conserved or variable epitopes was studied. 23 ART-naive subjects were characterized for CTL responses for 6 months, postinfection. While most CTL responses (73/123) were targeted against variable HIV-1 epitopes, recognition of conserved epitopes (16/123) was associated with lower set point viremia. This was independent of HLA type and also functional avidity of the CTL response. Most of the conserved responses were targeted to Gag and Pol epitopes.
• DRFYKTLRAEQ, restricted by B*1402, was recognized by 1 patient with the restricting allele.

References

Kunwar2013 Pratima Kunwar, Natalie Hawkins, Warren L. Dinges, Yi Liu, Erin E. Gabriel, David A. Swan, Claire E. Stevens, Janine Maenza, Ann C. Collier, James I. Mullins, Tomer Hertz, Xuesong Yu, and Helen Horton. Superior Control of HIV-1 Replication by CD8+ T Cells Targeting Conserved Epitopes: Implications for HIV Vaccine Design. PLoS One, 8(5):e64405, 2013. PubMed ID: 23741326. Show all entries for this paper.