Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 329

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HXB2 Location	Gag(298-306) p24(166-174) DNA(1681..1707)	Gag Epitope Map
Author Location	p24
Epitope	DRFYKLTRA	Epitope Alignment
Species (MHC/HLA)	human(B14)
Immunogen
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), HIV-2

Notes

• CTL responses in seronegative highly HIV-exposed African female sex workers in Gambia and Nairobi were studied -- these women had no delta 32 deletion in CCR5.
• In Gambia there is exposure to both HIV-1 and HIV-2. CTL responses to B*35 epitopes in exposed, uninfected women are cross-reactive, and the B*35 allele seems to be protective.
• HIV-2 sequence: DRFYKSLRA is cross-reactive, Ref. from authors is Harrer et al. J. Immunol. Vol. 161(9): pp 4875-81 (1993).
• Database note: First author "Rowland-Jones" is also found as "RowlandJones" on annotated papers in this database.

References

Rowland-Jones1999 S. L. Rowland-Jones, T. Dong, L. Dorrell, G. Ogg, P. Hansasuta, P. Krausa, J. Kimani, S. Sabally, K. Ariyoshi, J. Oyugi, K. S. MacDonald, J. Bwayo, H. Whittle, F. A. Plummer, and A. J. McMichael. Broadly Cross-Reactive HIV-Specific Cytotoxic T Lymphocytes in Highly-Exposed Persistently Seronegative Donors. Immunol. Lett., 66:9-14, 1999. PubMed ID: 10203028. Show all entries for this paper.

Displaying record number 54537

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HXB2 Location	Gag(298-306) p24(166-174) DNA(1681..1707)	Gag Epitope Map
Author Location	p24(166-174)
Epitope	DRFYKLTRA	Epitope Alignment
		Show epitope variants
Epitope Name	DA9
Subtype	B
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	binding affinity, subtype comparisons, acute/early infection

Notes

• T-cell responses to clade B Gag peptides were tested using sequences based on consensus (CON), most recent common ancestor (ANC) and center of tree (COT); as well as CON sequences from clade A, clade C and M-group Gag. It was found that Gag-specific cross-clade T-cell IFN-gamma responses are detectable during primary infection, and there was no significant difference in breadth or magnitude of responses with the different peptide sets. While several epitopes in low diversity domains are invariant across clades, other recognized variants are seen outside HLA anchor positions. T-cell immunity often tolerates conservative or semi-conservative amino acid substitutions but changes presumed to be in TCR contact sites may abrogate epitope recognition.
• Epitope sequences for this epitope, DA9 are invariant between CON B, COT B, ANC B and M-group. Cross-recognition is seen to both A- and C-clade variants. An anchor residue is at position 2; while both A- and C- variants contain a change at position 4 to DRFfKLTRA. HLA-B14 restriction was inferred based on 2 subjects possessing appropriate HLA class I allele and prior publication.

References

Malhotra2007 Uma Malhotra, Jessica Nolin, James I. Mullins, and M. Juliana McElrath. Comprehensive Epitope Analysis of Cross-Clade Gag-Specific T-Cell Responses in Individuals with Early HIV-1 Infection in the US Epidemic. Vaccine, 25(2):381-390, 4 Jan 2007. PubMed ID: 17112643. Show all entries for this paper.