Search CTL/CD8+ T-Cell Epitope Database

Found 6 matching records:

Displaying record number 53768

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HXB2 Location	Nef(86-94) DNA(9052..9078)	Nef Epitope Map
Author Location	Nef(86-94 HXB2)
Epitope	`DLSHFLKEK`	Epitope Alignment
		Show epitope variants
Subtype	B, CRF01_AE
Species (MHC/HLA)	human(A03, A11)
Immunogen	HIV-1 infection
Country	Viet Nam
Experimental methods	HLA binding
Keywords	subtype comparisons, computational epitope prediction, variant cross-reactivity, vaccine antigen design

Notes

The most common HLA-alleles in the Vietnamese population were found to be HLA-A*11, A*02, A*33, B*75, B*46, and B*62. Several epitopes cluster in short regions in Gag and Nef that are presented by these HLA molecules; these epitopes may be useful targets for vaccine antigens. In a comparison of CRF01_AE sequences to HXB2, 14 mutations were found in epitopes in these Gag and Nef pluriepitopic regions; 7 did not affect the HLA binding score, 3 increased it, and 4 reduced it.
The CRF01_AE variant DLSfFLKEK had same HLA-binding score as the HXB2 epitope.

References

Lazaro2005 Estibaliz Lazaro, Ioannis Theodorou, Elisabeth Legrand, Patricia Recordon-Pinson, Sebastien Boucher, Corinne Capoulade, Thi Hoang Lan, Pham Van Hung, Patrice Debre, and Herve Fleury. Sequences of Clustered Epitopes in Gag and Nef Potentially Presented by Predominant Class I Human Leukocyte Antigen (HLA) Alleles A and B Expressed By Human Immunodeficiency Virus Type 1 (HIV-1)-Infected Patients in Vietnam. AIDS Res. Hum. Retroviruses, 21(6):586-591, Jun 2005. PubMed ID: 15989466. Show all entries for this paper.

Displaying record number 1009

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HXB2 Location	Nef(86-94) DNA(9052..9078)	Nef Epitope Map
Author Location	Nef(84-92 LAI)
Epitope	`DLSHFLKEK`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*03:01)
Immunogen	HIV-1 infection
Experimental methods
Keywords	review

Notes

Review of HIV CTL epitopes.

References

McMichael1994 A. J. McMichael and B. D. Walker. Cytotoxic T Lymphocytes Epitopes: Implications for HIV Vaccine. AIDS, 8S:S155-S173, 1994. Comprehensive review summarizing CTL epitopes that have known HLA type and are fine mapped to indicate epitope boundaries. Anchor residues are indicated when known for different HLA restricted epitopes. Includes a summary of the published literature, as well as much work that was in press or submitted for publication. Show all entries for this paper.

Displaying record number 52437

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HXB2 Location	Nef(86-94) DNA(9052..9078)	Nef Epitope Map
Author Location	Nef
Epitope	`DLSHFLKEK`	Epitope Alignment
Subtype	A, B, D, F
Species (MHC/HLA)	human, macaque(A*03:01)
Immunogen	HIV-1 infection, vaccine
Experimental methods
Keywords	subtype comparisons, epitope processing, vaccine-induced immune responses, immunodominance

Vaccine Details

Vaccine type	DNA prime with modified vaccinia Ankara (MVA) boost
Vaccine strain	A clade
Vaccine component	p17 Gag, p24 Gag

Notes

The HIV-1 subtype A focused vaccine HIVA contains p24 and p17, in a reversed order relative to the Gag polyprotein to prevent myristylation of p17, which could direct the protein to the cell membrane and inhibit efficient peptide processing and class I presentation, as well as a polyepitope string of conserved, often immunodominant epitopes that were selected to have particularly good cross-reactive potential for the A-clade epidemic in Nairobi, Kenya. A DNA and MVA prime-boost vaccination protocol using the HIVA antigen will be used in a phase III clinical trial in Kenya. This epitope is included in the polyepitope string Hanke2000.
Multiple CD4+ or CD8+ T-cell vaccine-induced responses to peptide pools were detected using intracellular cytokine staining and IFNgamma Elispot assays after vaccination of 5 macaques. The response to the Mamu A*01 SIV p27 epitope p11C (CTPYDINQM), included in the polyepitope region, was not immunodominant in the Mamu A*01 vaccinated macaques, possibly because of processing limitations in context of the artificial polyepitope string Wee2002.

References

Hanke2000 Tomas Hanke and Andrew J. McMichael. Design and construction of an experimental HIV-1 vaccine for a year-2000 clinical trial in Kenya. Nat. Med., 6(9):951-955, Sep 2000. PubMed ID: 10973301. Show all entries for this paper.

Wee2002 Edmund G.-T. Wee, Sandip Patel, Andrew J. McMichael, and Tomáš Hanke. A DNA/MVA-Based Candidate Human Immunodeficiency Virus Vaccine for Kenya Induces Multi-Specific T Cell Responses in Rhesus Macaques. J. Gen. Virol., 83(Pt 1):75-80, Jan 2002. PubMed ID: 11752703. Show all entries for this paper.

Displaying record number 57099

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HXB2 Location	Nef(86-94) DNA(9052..9078)	Nef Epitope Map
Author Location	Nef(86-94 DU422)
Epitope	`DLSHFLKEK`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*03:01)
Immunogen	in vitro stimulation or selection
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Tetramer binding
Keywords	dendritic cells

Notes

Three types of myeloid dendritic cells - monocytic dendritic cells, Langerhans cells, and interstitial dermal-intestinal dendritic cells - were shown to be able to prime CD8+ T-cell responses to HIV-1. Primed monocytic dendritic cells from 2 uninfected adult donors had broad primary T-cell reactivity, reacting with 15mers containing 42 known epitopes in Gag, Env, and Nef.
One of the reactive 15mers contained optimal epitope DLSHFLKEK, which was restricted by HLA A*0301.

References

Colleton2009 Bonnie A. Colleton, Xiao-Li Huang, Nada M. Melhem, Zheng Fan, Luann Borowski, Giovanna Rappocciolo, and Charles R. Rinaldo. Primary Human Immunodeficiency Virus Type 1-Specific CD8+ T-Cell Responses Induced by Myeloid Dendritic Cells. J. Virol., 83(12):6288-6299, Jun 2009. PubMed ID: 19357176. Show all entries for this paper.

Displaying record number 53017

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HXB2 Location	Nef(86-94) DNA(9052..9078)	Nef Epitope Map
Author Location	Nef(86-94)
Epitope	`DLSHFLKEK`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*03:01, A11)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, CD8 T-cell Elispot granzyme B
Keywords	Th1, characterizing CD8+ T cells

Notes

Only 20% of CD8+ T-cells produce IFN-gamma and granzyme B simultaneously (Tc1a). Two additional subpopulations of HIV specific CD8 cells are found, each one constituting 30-40% of the CD8 cell pool. One of these (Tc1b) secretes IFN-gamma only, and the other one (Tc1c) secretes GzB only.
Two of nine patients responded to this peptide with GzB producing cells, while three of the patients responded with IFN-gamma producing cells. Only one patient had both GzB and IFN-gamma responses.

References

Kleen2004 Thomas O. Kleen, Robert Asaad, Samuel J. Landry, Bernhard O. Boehm, and Magdalena Tary-Lehmann. Tc1 Effector Diversity Shows Dissociated Expression of Granzyme B and Interferon-gamma in HIV Infection. AIDS, 18(3):383-392, 20 Feb 2004. PubMed ID: 15090789. Show all entries for this paper.

Displaying record number 1655

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HXB2 Location	Nef(86-94) DNA(9052..9078)	Nef Epitope Map
Author Location	Nef(86-94)
Epitope	`DLSHFLKEK`	Epitope Alignment
Species (MHC/HLA)	human(A3)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Kaul2001a R. Kaul, T. Dong, F. A. Plummer, J. Kimani, T. Rostron, P. Kiama, E. Njagi, E. Irungu, B. Farah, J. Oyugi, R. Chakraborty, K. S. MacDonald, J. J. Bwayo, A. McMichael, and S. L. Rowland-Jones. CD8(+) lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J. Clin. Invest., 107(10):1303-10, May 2001. URL: http://www.jci.org/cgi/content/full/107/10/1303. PubMed ID: 11375420. Show all entries for this paper.