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Displaying record number 58483

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HXB2 Location	Gag(329-337) p24(197-205) DNA(1774..1800)	Gag Epitope Map
Author Location	p24(197-205)
Epitope	DCKSILRAL	Epitope Alignment
Subtype	A1
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Kenya
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Proliferation
Keywords	assay or method development, characterizing CD8+ T cells

Notes

• 24 longitudinally studied HIV-1 (clades A1 or D)-chronically-infected Kenyan subjects were tested for CTL response to 9-mer overlapping p24 peptides. Epitope mapping was assessed not just by IFN-γ EliSpot assays, but by multiparametric flow cytometry of intracellular cytokine staining for IL-2, tumor necrosis factor (TNF-α), chemokine macrophage inflammatory protein 1β (MIP-1β) and degranulation marker CD107a, as well as by proliferation assays. 50/69 epitope-specific responses detected were IFN-γ negative. Several epitope responses had polyfunctional (33%) and proliferative (19%) components. Long term non progressors (LTNP) had more polyfunctional responses of greater magnitude and proliferation than normal progressors (NP).
• 16 subjects were tested specifically with 126 putative epitopes, 50 of which were confirmed. Clade A1 epitope DCKsILrAL, corresponds to B consensus DCKTILKAL, and elicited a single proliferative response to an NP. This epitope is or is contained within a best-defined epitope.

References

Richmond2011 Meika Richmond, Lyle R. McKinnon, Sandra A. Koesters Kiazyk, Charles Wachihi, Makobu Kimani, Joshua Kimani, Francis A. Plummer, and T. Blake Ball. Epitope Mapping of HIV-Specific CD8+ T Cell Responses by Multiple Immunological Readouts Reveals Distinct Specificities Defined by Function. J. Virol., 85(3):1275-1286, Feb 2011. PubMed ID: 21084478. Show all entries for this paper.

Displaying record number 58939

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HXB2 Location	Gag(329-337) p24(197-205) DNA(1774..1800)	Gag Epitope Map
Author Location	Gag
Epitope	DCKSILRAL	Epitope Alignment
Subtype	A
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Kenya, Pakistan
Experimental methods	Other
Keywords	epitope processing

Notes

• To compare epitopes generated in diverse genetic populations due to the mutations that affect respective antigen processing, 29 Kenyan and 56 Pakistani subjects infected with HIV-1 subtype A were studied. HIV-1 Gag was sequenced for mutations affecting proteasomal degradation, TAP or HLA binding and CTL epitope generation. Epitope patterns were found to be altered due to population-specific mutations in epitope-generating pathways.
• Several predicted epitopes of differential frequency were shown in this study (see Table 1). Only epitopes immunologically verified previously are annotated here.
• Epitope DCKSILRAL was generated from HIV-1 subtype A for HLA-presentation in Kenyan but not Pakistani populations.

References

Abidi2013 Syed H. Abidi, Aniqa Shahid, Laila S. Lakhani, Muhammad R. Khanani, Peter Ojwang, Nancy Okinda, Reena Shah, Farhat Abbas, Sarah Rowland-Jones, and Syed Ali. Population-Specific Evolution of HIV Gag Epitopes in Genetically Diverged Patients. Infect. Genet. Evol., 16:78-86, Jun 2013. PubMed ID: 23403357. Show all entries for this paper.