Search CTL/CD8+ T-Cell Epitope Database

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Displaying record number 689

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HXB2 Location	gp160(239-247) gp120(239-247) DNA(6939..6965)	gp160 Epitope Map
Author Location	gp120(241-249 LAI)
Epitope	CTNVSTVQC	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

• HIV IIIB proteins were used to define the range of CTL epitopes recognized by 3 lab workers accidentally infected with HIV-1 IIIB.
• CTNVSTVQC contains a potential N-linked glycosylation site and cysteine residues, possibly related to a requirement for a high sensitizing dose of peptide for CTL activity.

References

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.

Displaying record number 55294

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HXB2 Location	gp160(239-247) gp120(239-247) DNA(6939..6965)	gp160 Epitope Map
Author Location	Env
Epitope	CTNVSTVQC	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Patient MHC/HLA	A1, A19, B*35:01, B44, C*16, Cw7; A*02:01, A19, B14, B44, C*16, Cw8
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	HAART, ART, mother-to-infant transmission, rate of progression, escape, co-receptor, mutation acquisition, HLA associated polymorphism

Notes

• HIV-1 mother-to-child transmission is studied for LTNPs by comparing entire genomes from 2 mother (M1, M2) and 2 daughter (D1, D2) RNA samples of a mother-child pair over 11 years. Genetic distance was 94% between subjects' strains. Divergence in sequences was attributed to distinct HLA selection pressures as ds/dn was larger for intra- rather than inter-person sequences. 10 new mutations in D2 were found related to unique daughter HLA alleles.
• Functional ELISpot studies using D2 and Nef peptides reveal strong associations between CTL responses and escape variants, contributing to delayed progression.
• LTNP status was not related to defective virus since all viral genes were intact and CTL response did not effectively control viral load. It is supposed that genetic HLA background and HIV-1 epitope-immune response interaction account for nonprogression of disease.
• All isolates contained R77Q in Vpr, a variation associated with reduction of cellular apoptosis.
• This HLA-Cw8 restricted epitope, CTNVSTVQC was mutated to CqNVSTVQC in the daughter D2 isolate.

References

Reinis2007 Milan Reinis, Barbara Weiser, Carla Kuiken, Tao Dong, Dorothy Lang, Sharon Nachman, Yonghong Zhang, Sarah Rowland-Jones, and Harold Burger. Genomic Analysis of HIV Type 1 Strains Derived from a Mother and Child Pair of Long-Term Nonprogressors. AIDS Res. Hum. Retroviruses, 23(2):309-315, Feb 2007. PubMed ID: 17331038. Show all entries for this paper.