Search CTL/CD8+ T-Cell Epitope Database

Found 6 matching records:

Displaying record number 52268

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HXB2 Location	Gag(405-413) p2p7p1p6(42-50) DNA(2002..2028)	Gag Epitope Map
Author Location	p15(42-50 SF2)
Epitope	CRAPRKKGC	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Patient MHC/HLA	B14
Experimental methods
Keywords	immunodominance

Notes

• 26/57 HIV-1 infected subjects displayed Gag p15-specific CD8+ T-cell IFNgamma responses were measured by Elispot and intracellular staining. The immunodominant regions targeted by CD8+ T-cells were mapped to three functional domains: the zinc finger structures, the protease cleavage site p7/p1, and to the Vpr binding site in p6.
• p15 contributed on average 17% of the total Gag response (rage 0-100%).
• 3 optimal CTL epitopes were mapped within p15: KELYPLTSL, CRAPRKKGC, and FLGKIWPSYK.
• 2/6 HLA-B14+ subjects recognized this epitope. The binding motif for B14 is C-term Cys, positions 2 and 5 Arg.

References

Yu2002a Xu G. Yu, Hong Shang, Marylyn M. Addo, Robert L. Eldridge, Mary N. Phillips, Margaret E. Feeney, Daryld Strick, Christian Brander, Philip J. R. Goulder, Eric S. Rosenberg, Bruce D. Walker, Marcus Altfeld, and HIV Study Collaboration. Important Contribution of p15 Gag-Specific Responses to the Total Gag-Specific CTL Responses. AIDS, 16(3):321-328, 15 Feb 2002. PubMed ID: 11834942. Show all entries for this paper.

Displaying record number 52552

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HXB2 Location	Gag(405-413) p2p7p1p6(42-50) DNA(2002..2028)	Gag Epitope Map
Author Location	p15(42-50)
Epitope	CRAPRKKGC	Epitope Alignment
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope, A-list

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 53366

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HXB2 Location	Gag(405-413) p2p7p1p6(42-50) DNA(2002..2028)	Gag Epitope Map
Author Location	( B consensus)
Epitope	CRAPRKKGC	Epitope Alignment
Epitope Name	CC9
Subtype	B
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Patient MHC/HLA	A28, A29, B14, B44, Cw8
Experimental methods	Chromium-release assay, Cytokine production, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	assay or method development, memory cells, characterizing CD8+ T cells

Notes

• Using a flow-cytometric cytotoxicity assay based on caspase-3 activation in dying target cells, it was shown that the subset of HIV-1-specific CD8+ T cells secreting both IFN-gamma and TNF-alpha exhibit stronger cytotoxic activity than those secreting only IFN-gamma. These cells also exhibited stronger intracellular perforin expression. No association between HIV-1-specific CD8+ T-cell maturation phenotypes and intracellular perforin expression was found.
• 1/9 individuals recognized this epitope

References

Lichterfeld2004b Mathias Lichterfeld, Xu G. Yu, Michael T. Waring, Stanley K. Mui, Mary N. Johnston, Daniel Cohen, Marylyn M. Addo, John Zaunders, Galit Alter, Eunice Pae, Daryld Strick, Todd M. Allen, Eric S. Rosenberg, Bruce D. Walker, and Marcus Altfeld. HIV-1-Specific Cytotoxicity Is Preferentially Mediated by a Subset of CD8+ T Cells Producing both Interferon-gamma and Tumor Necrosis Factor-alpha. Blood, 104(2):487-394, 15 Jul 2004. PubMed ID: 15059848. Show all entries for this paper.

Displaying record number 53569

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HXB2 Location	Gag(405-413) p2p7p1p6(42-50) DNA(2002..2028)	Gag Epitope Map
Author Location	Gag(405-413)
Epitope	CRAPRKKGC	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Patient MHC/HLA	A28, A29, B14, B44, Cw8
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords

Notes

• Two-thirds of all mutations arising in non-envelope proteins during the transition from acute to chronic infection were found to be due to either CD8 T-cell-associated selective pressures or reversion of HLA-I-associated mutations selected in a previous host.The predominance of escape mutations at highly polymorphic sites was observed across different HIV-1 clades.
• This epitope was reactive, but escape mutations did not accrue in it over time.

References

Allen2005a Todd M. Allen, Marcus Altfeld, Shaun C. Geer, Elizabeth T. Kalife, Corey Moore, Kristin M. O'Sullivan, Ivna DeSouza, Margaret E. Feeney, Robert L. Eldridge, Erica L. Maier, Daniel E. Kaufmann, Matthew P. Lahaie, Laura Reyor, Giancarlo Tanzi, Mary N. Johnston, Christian Brander, Rika Draenert, Jurgen K. Rockstroh, Heiko Jessen, Eric S. Rosenberg, Simon A. Mallal, and Bruce D. Walker. Selective Escape from CD8+ T-Cell Responses Represents a Major Driving Force of Human Immunodeficiency Virus Type 1 (HIV-1) Sequence Diversity and Reveals Constraints on HIV-1 Evolution. J. Virol., 79(21):13239-13249, Nov 2005. PubMed ID: 16227247. Show all entries for this paper.

Displaying record number 59563

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HXB2 Location	Gag(405-413) p2p7p1p6(42-50) DNA(2002..2028)	Gag Epitope Map
Author Location	Gag(405-413)
Epitope	CRAPRKKGC	Epitope Alignment
Epitope Name	CC9
Species (MHC/HLA)	human(C*08)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

• Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
• CRAPRKKGC had a significantly positive association with HLA C*08; it was recognized by 8/41 C*08-positive subjects and 1/300 C*08-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 62829

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HXB2 Location	Gag(405-413) p2p7p1p6(42-50) DNA(2002..2028)	Gag Epitope Map
Author Location	p2p7p1p6(405-413)
Epitope	CRAPRKKGC	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	engineered
Experimental methods	Other, Relative replication capacity assay
Keywords	assay or method development, protective allele

Notes

• NL4-3 Gag polyprotein random mutagenesis, high-throughput fitness profiling and in silico prediction of MHC-peptide binding affinity revealed that mutations in CTL epitopes presented by protective MHC-I alleles, vs. non-protective alleles, showed greater loss of experimental replicative capacity and lower levels of predicted reduction in MHC-I binding affinity. Together, these two variables could predict protectiveness of MHC-I alleles in a linear regression model. Analyses of intraperson viral evolution in 4 LTNP vs. 4 paired progressor subjects further supported paper conclusions.
• After mutagenesis and passaging through human T cells, 37 single nucleotide mutations were observed in epitope CRAPRKKGC (known to bind to B1402) with a log transformation of mean fitness score, relative to NL4-3, of -0.3755.

References

Du2017 Yushen Du, Tian-Hao Zhang, Lei Dai, Xiaojuan Zheng, Aleksandr M. Gorin, John Oishi, Ting-Ting Wu, Janice M. Yoshizawa, Xinmin Li, Otto O. Yang, Otoniel Martinez-Maza, Roger Detels, and Ren Sun. Effects of Mutations on Replicative Fitness and Major Histocompatibility Complex Class I Binding Affinity Are Among the Determinants Underlying Cytotoxic-T-Lymphocyte Escape of HIV-1 Gag Epitopes. mBio, 8(6), 28 Nov 2017. PubMed ID: 29184023. Show all entries for this paper.