Search CTL/CD8+ T-Cell Epitope Database

Found 15 matching records:

Displaying record number 59561

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37)
Epitope	`CCFHCQVC`	Epitope Alignment
Epitope Name	CC8
Species (MHC/HLA)	human(B*18)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
CCFHCQVC had a significantly positive association with HLA B*18; it was recognized by 1/6 B*18-positive subjects and 0/189 B*18-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 59562

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37)
Epitope	`CCFHCQVC`	Epitope Alignment
Epitope Name	CC8
Species (MHC/HLA)	human(C*07)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
CCFHCQVC had a significantly positive association with HLA C*07; it was recognized by 4/96 C*07-positive subjects and 1/195 C*07-negative subjects.

References

Displaying record number 53530

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat
Epitope	`CCFHCQVC`	Epitope Alignment
Epitope Name	CC8
Subtype	B
Species (MHC/HLA)	human(C*12)
Immunogen	HIV-1 infection
Patient MHC/HLA	A11, A2, B18, B44, C*12, Cw5
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, escape, characterizing CD8+ T cells, viral fitness and reversion

Notes

Two-thirds of all mutations arising in non-envelope proteins during the transition from acute to chronic infection were found to be due to either CD8 T-cell-associated selective pressures or reversion of HLA-I-associated mutations selected in a previous host.The predominance of escape mutations at highly polymorphic sites was observed across different HIV-1 clades.
One escape mutation, at position 3, CClHCQVC, was found in the most polymorphic residue in the epitope.

References

Allen2005a Todd M. Allen, Marcus Altfeld, Shaun C. Geer, Elizabeth T. Kalife, Corey Moore, Kristin M. O'Sullivan, Ivna DeSouza, Margaret E. Feeney, Robert L. Eldridge, Erica L. Maier, Daniel E. Kaufmann, Matthew P. Lahaie, Laura Reyor, Giancarlo Tanzi, Mary N. Johnston, Christian Brander, Rika Draenert, Jurgen K. Rockstroh, Heiko Jessen, Eric S. Rosenberg, Simon A. Mallal, and Bruce D. Walker. Selective Escape from CD8+ T-Cell Responses Represents a Major Driving Force of Human Immunodeficiency Virus Type 1 (HIV-1) Sequence Diversity and Reveals Constraints on HIV-1 Evolution. J. Virol., 79(21):13239-13249, Nov 2005. PubMed ID: 16227247. Show all entries for this paper.

Displaying record number 53789

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat
Epitope	`CCFHCQVC`	Epitope Alignment
Epitope Name	CC8
Species (MHC/HLA)	(C*12)
Immunogen
Experimental methods
Keywords	review, immunodominance, escape, acute/early infection, early-expressed proteins, kinetics, viral fitness and reversion

Notes

This review discusses the importance of 3 factors that impact the selection of immunodominant epitopes in acute HIV infection: i) the kinetics of viral protein expression, ii) the HLA class I background of the infected individual, and iii) the autologous sequence of the infecting virus. This is 1 of 12 peptides listed as immunodominant in acute HIV-1 infection.

References

Lichterfeld2005 Mathias Lichterfeld, Xu G. Yu, Sylvie Le Gall, and Marcus Altfeld. Immunodominance of HIV-1-Specific CD8+ T-Cell Responses in Acute HIV-1 Infection: At the Crossroads of Viral and Host Genetics. Trends Immunol., 26(3):166-171, Mar 2005. PubMed ID: 15745859. Show all entries for this paper.

Displaying record number 52611

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37)
Epitope	`CCFHCQVC`	Epitope Alignment
Species (MHC/HLA)	human(C*12)
Immunogen
Experimental methods
Keywords	optimal epitope, A-list

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 53476

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37)
Epitope	`CCFHCQVC`	Epitope Alignment
		Show epitope variants
Epitope Name	CC8
Subtype	B
Species (MHC/HLA)	human(C*12)
Immunogen	HIV-1 infection
Patient MHC/HLA	A11, A2, B18, B44, C*12, Cw5
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	escape

Notes

4/14 optimal HIV-1 T-cell epitopes in a subject underwent mutation associated with dramatic loss of the original CD8 response. For 1 of the escape variants, a novel CD8 T-cell response equal in magnitude to the wildtype, was generated. CD8 T-cell recognizing the variant epitope utilized a distinct T-cell receptor and did not exhibit any cross-reactivity against the wildtype.
A mutation occurred at position 3 of this epitope, ccMhcqvc, but significant cross-recognition was observed between the escape variant and the wildtype epitope.

References

Allen2005 Todd M. Allen, Xu G. Yu, Elizabeth T. Kalife, Laura L. Reyor, Mathias Lichterfeld, Mina John, Michael Cheng, Rachel L. Allgaier, Stanley Mui, Nicole Frahm, Galit Alter, Nancy V. Brown, Mary N. Johnston, Eric S. Rosenberg, Simon A. Mallal, Christian Brander, Bruce D. Walker, and Marcus Altfeld. De Novo Generation of Escape Variant-Specific CD8+ T-Cell Responses Following Cytotoxic T-Lymphocyte Escape in Chronic Human Immunodeficiency Virus Type 1 Infection. J. Virol., 79(20):12952-12960, Oct 2005. PubMed ID: 16188997. Show all entries for this paper.

Displaying record number 57173

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location
Epitope	`CCFHCQVC`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(C*12)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	immunodominance, acute/early infection

Notes

To compare the prevalence and immunodominance of CTL responses to conserved and variable epitopes, CTL responses were analyzed in 45 HIV-1 B-infected males during primary and/or chronic infection. Conserved and variable CTL epitopes were recognized with similar probabilities, but conserved epitopes generally elicited subdominant responses. Low viral loads were associated with Gag-specific responses during acute infection, but with Env-specific responses during chronic infection.
Among 8 Cw12-positive subjects tested during primary infection, 8 recognized CCFHCQVC, and 7 had an immunodominant response to it. Among 5 Cw12-positive subjects tested during chronic infection, 3 recognized CCFHCQVC, and 1 had an immunodominant response to it.

References

Liu2009c Yi Liu, John McNevin, Morgane Rolland, Hong Zhao, Wenjie Deng, Janine Maenza, Claire E. Stevens, Ann C. Collier, M. Juliana McElrath, and James I. Mullins. Conserved HIV-1 Epitopes Continuously Elicit Subdominant Cytotoxic T-Lymphocyte Responses. J. Infect. Dis., 200(12):1825-1833, 15 Dec 2009. PubMed ID: 19909083. Show all entries for this paper.

Displaying record number 59560

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37)
Epitope	`CCFHCQVC`	Epitope Alignment
Epitope Name	CC8
Species (MHC/HLA)	human(C*12)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
CCFHCQVC had a significantly positive association with HLA C*12; it was recognized by 9/50 C*12-positive subjects and 5/291 C*12-negative subjects.

References

Displaying record number 52640

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37)
Epitope	`CCFHCQVC`	Epitope Alignment
Species (MHC/HLA)	human(C*12:03)
Immunogen	HIV-1 infection
Patient MHC/HLA	A26, A3, B38:01, B7, C07:02, C12:03; A02:01, A25:01, B18, B51, C01:02, C*12:03
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	acute/early infection, early treatment

Notes

CTL epitope responses were mapped in 21 men within 15-92 days post-HIV-1 infection. Subjects initially showed a narrow immune response with a mean of 2.3 epitopes recognized per patient. The immune response broadened later in infection. No correlation between the plasma viral load and the number of recognized epitopes or the frequency of IFNγ-secreting cells was observed, and there was no correlation between the functional avidity of a response and the abundance of IFNγ-secreting cells that recognized the epitope. The first epitopes to be recognized tended not to be the most avid, and earliest responses tended to be directed against Nef, Tat, Vpr, and Env.
Two individuals recognized this epitope both presented by Cw*1203.
More common HLA alleles were less frequently used in primary infection than less common alleles, for example A3, B35, B57, and B62 were more frequently recognized than A1, A2, A30, and A44.
All HIV-1 proteins except Vpu were recognized, and responses to a total of 41 optimal epitopes were characterized; 24 had been identified previously. 48% of the total T-cell responses were directed against Nef and 43% were directed against Gag.

References

Cao2003 Jianhong Cao, John McNevin, Sarah Holte, Lisa Fink, Lawrence Corey, and M. Juliana McElrath. Comprehensive Analysis of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific Gamma Interferon-Secreting CD8+ T Cells in Primary HIV-1 Infection. J. Virol., 77(12):6867-6878, Jun 2003. PubMed ID: 12768006. Show all entries for this paper.

Displaying record number 58146

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37)
Epitope	`CCFHCQVC`	Epitope Alignment
		Show epitope variants
Epitope Name	CC8
Subtype	B
Species (MHC/HLA)	human(C*12:03)
Immunogen	HIV-1 infection
Patient MHC/HLA	PIC1362: A02:01, A25:01, B18:01, B51:01, C01:02, C12:03
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	escape, acute/early infection

Notes

CTL responses were studied in a drug-naive patient for 4 years, beginning at the time of infection. CTL recognition of 25 epitopes was observed, and 18 of them underwent mutational changes, usually indicative of escape.
Evolving epitope CCFHCQVC underwent strong selection during 4 years of infection.
Variant CCFHCQsC arose early in infection, then disappeared, while CClHCQVC dominated by 200 days post-infection. Both variants were associated with loss of EliSpot response to the epitope.

References

Liu2011a Yi Liu, John P. McNevin, Sarah Holte, M. Juliana McElrath, and James I. Mullins. Dynamics of Viral Evolution and CTL Responses in HIV-1 Infection. PLoS One, 6(1):e15639, 2011. PubMed ID: 21283794. Show all entries for this paper.

Displaying record number 58991

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37)
Epitope	`CCFHCQVC`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(C*12:03)
Immunogen	HIV-1 infection
Country	Australia, Canada, Spain, Switzerland
Experimental methods	Other
Keywords	mutation acquisition

Notes

Computational genome-wide scans were run to find associations between (subjects' ) host genome DNA polymorphisms, and infecting HIV-1 genome sequence variation from 1071 subjects, as well as their viral load (VL). Such genome-to-genome approaches also reveal sites of single nucleotide polymorphisms (SNPs) linked to variant HIV-1 amino acids that either limit viral efficiency or that are easily tolerated by it.
48 amino acid residues of HIV-1 were linked to human SNPs, and the variants were not antiretroviral drug resistance mutations. 50% of these viral amino acids were located within previously defined optimal epitopes, such as CCFHCQVC, whose variation at position 32 is associated with SNP rs16899214.

References

Bartha2013 István Bartha, Jonathan M. Carlson, Chanson J. Brumme, Paul J. McLaren, Zabrina L. Brumme, Mina John, David W. Haas, Javier Martinez-Picado, Judith Dalmau, Cecilio López-Galíndez, Concepción Casado, Andri Rauch, Huldrych F. Günthard, Enos Bernasconi, Pietro Vernazza, Thomas Klimkait, Sabine Yerly, Stephen J. O'Brien, Jennifer Listgarten, Nico Pfeifer, Christoph Lippert, Nicolo Fusi, Zoltán Kutalik, Todd M. Allen, Viktor Müller, P. Richard Harrigan, David Heckerman, Amalio Telenti, and Jacques Fellay. A Genome-to-Genome Analysis of Associations between Human Genetic Variation, HIV-1 Sequence Diversity, and Viral Control. eLife, 2:e01123, 2013. PubMed ID: 24171102. Show all entries for this paper.

Displaying record number 59089

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37)
Epitope	`CCFHCQVC`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(C*12:03)
Immunogen	HIV-1 infection
Patient MHC/HLA	PIC1362: A02:01, A25:01, B18:01, B51:01, C01:02, C12:03
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	viral fitness and reversion

Notes

CTL responses were studied in a single drug-naive individual during the first 4 years of infection. 25 CTL epitopes were identified; 17 of them underwent evolution; 15 mutant forms that escaped or partially escaped were detected.
CCFHCQVC was an epitope whose frequency decreased during 4 years of infection; 2 escape mutants were detected (CClHCQVC, CCFHCQsC).

References

Liu2007a Yi Liu, John McNevin, Hong Zhao, Denis M. Tebit, Ryan M. Troyer, Matthew McSweyn, Ananta K. Ghosh, Daniel Shriner, Eric J. Arts, M. Juliana McElrath, and James I. Mullins. Evolution of Human Immunodeficiency Virus Type 1 Cytotoxic T-Lymphocyte Epitopes: Fitness-Balanced Escape. J. Virol., 81(22):12179-12188, Nov 2007. PubMed ID: 17728222. Show all entries for this paper.

Displaying record number 52639

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37)
Epitope	`CCFHCQVC`	Epitope Alignment
Species (MHC/HLA)	human(C*12:03)
Immunogen	HIV-1 infection
Patient MHC/HLA	A26, A3, B38:01, B7, C07:02, C12:03; A02:01, A25:01, B18, B51, C01:02, C*12:03
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	acute/early infection, early treatment

Notes

CTL epitope responses were mapped in 21 men within 15-92 days post-HIV-1 infection. Subjects initially showed a narrow immune response with a mean of 2.3 epitopes recognized per patient. The immune response broadened later in infection. No correlation between the plasma viral load and the number of recognized epitopes or the frequency of IFNγ-secreting cells was observed, and there was no correlation between the functional avidity of a response and the abundance of IFNγ-secreting cells that recognized the epitope. The first epitopes to be recognized tended not to be the most avid, and earliest responses tended to be directed against Nef, Tat, Vpr, and Env.
Two individuals recognized this epitope both presented by Cw*1203.
More common HLA alleles were less frequently used in primary infection than less common alleles, for example A3, B35, B57, and B62 were more frequently recognized than A1, A2, A30, and A44.
All HIV-1 proteins except Vpu were recognized, and responses to a total of 41 optimal epitopes were characterized; 24 had been identified previously. 48% of the total T-cell responses were directed against Nef and 43% were directed against Gag.

References

Displaying record number 54159

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location	Tat(30-37 HXB2)
Epitope	`CCFHCQVC`	Epitope Alignment
		Show epitope variants
Epitope Name	CC8
Subtype	B
Species (MHC/HLA)	human(C*12:03)
Immunogen	HIV-1 infection
Patient MHC/HLA	PIC1362: A02:01, A25:01, B18:01, B51:01, C01:02, C12:03
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	escape, mutation acquisition, optimal epitope

Notes

T-cell immunity and the impact of selection were longitudinally studied in a patient from the time of transmission. New epitopes were detected at sites that were shown to be under positive selection. Env was found to be the major target of CTLs and most of the new epitopes were found here. One third of the sites examined were associated with epitope escape mutations from CTL. Acquisition of mutations found at high frequency in the database was suggested to be a result of reversion of CTL epitopes in the absence of immune targeting.
Position 3 in the epitope had potentially experienced positive selection. CClHCQVC and CCFHCQsC escape variants were found.

References

Liu2006 Yi Liu, John McNevin, Jianhong Cao, Hong Zhao, Indira Genowati, Kim Wong, Sherry McLaughlin, Matthew D. McSweyn, Kurt Diem, Claire E. Stevens, Janine Maenza, Hongxia He, David C. Nickle, Daniel Shriner, Sarah E. Holte, Ann C. Collier, Lawrence Corey, M. Juliana McElrath, and James I. Mullins. Selection on the Human Immunodeficiency Virus Type 1 Proteome following Primary Infection. J. Virol., 80(19):9519-9529, Oct 2006. PubMed ID: 16973556. Show all entries for this paper.

Displaying record number 54925

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HXB2 Location	Tat(30-37) DNA(5918..5941)	Tat Epitope Map
Author Location
Epitope	`CCFHCQVC`	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	HIV-1 infection, vaccine
Patient MHC/HLA	014A-005: A25:01, A30:02, B07:02, B18:01, C07:02, C12:03
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	vaccine-induced immune responses, acute/early infection

Vaccine Details

Vaccine type	canarypox prime with gp120 boost
Vaccine strain	B clade LAI, B clade MN
Vaccine component	Gag-Pol, gp120, gp41

Notes

T cell responses during early infection in individuals previously enrolled in HIV vaccine trials were compared with those of nonvaccinated subjects.
None of the breakthrough infections possessed vaccine-induced T-cell responses preinfection and 85-86% of vaccinees and nonvaccinees with primary infection developed T-cell responses postinfection, indicating that vaccinees' ability to mount T-cell response postinfection is not compromized by previous immunization.
Breakthrough subjects recognized 43 T-cell epitopes, 8 of them novel, and 25% were present in the vaccine.
This epitope was not contained in the vaccine, but the linked vaccinated patient in whom the study was conducted recognized it after developing infection.

References

Horton2006a Helen Horton, Colin Havenar-Daughton, Deborah Lee, Erin Moore, Jianhong Cao, John McNevin, Thomas Andrus, Haiying Zhu, Abbe Rubin, Tuofu Zhu, Connie Celum, and M. Juliana McElrath. Induction of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific T-Cell Responses in HIV Vaccine Trial Participants Who Subsequently Acquire HIV-1 Infection. J. Virol., 80(19):9779-9788, Oct 2006. PubMed ID: 16973582. Show all entries for this paper.