Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 52938

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HXB2 Location	Nef(195-202) DNA(9379..9402)	Nef Epitope Map
Author Location	Nef(195-202)
Epitope	ARELHPEY	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A1)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Proliferation
Keywords	vaccine-induced immune responses, variant cross-reactivity

Vaccine Details

Notes

• After injection of an anti-HIV-1 lipopeptide vaccine and the booster injections, a response was observed in >85% of 28 volunteers, and a long-term immune response was observed in >50% of the volunteers. A positive effect of QS21 adjuvant was found for B-and CD4+T-cell responses. A higher number of CD8+ T-cell epitopes was induced after a fourth boost injection. This epitope was one of 31 that were recognized in the vaccinees.

References

Gahery-Segard2003 Hanne Gahéry-Ségard, Gilles Pialoux, Suzanne Figueiredo, Céline Igéa, Mathieu Surenaud, Jessintha Gaston, Helene Gras-Masse, Jean-Paul Lévy, and Jean-Gérard Guillet. Long-Term Specific Immune Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine: Characterization of CD8+-T-Cell Epitopes Recognized. J. Virol., 77(20):11220-11231, Oct 2003. PubMed ID: 14512570. Show all entries for this paper.

Displaying record number 53716

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HXB2 Location	Nef(195-202) DNA(9379..9402)	Nef Epitope Map
Author Location	Nef(195-202 BRU)
Epitope	ARELHPEY	Epitope Alignment
		Show epitope variants
Subtype	B, CRF02_AG
Species (MHC/HLA)	human(A1)
Immunogen	HIV-1 infection
Country	Cote D'Ivoire
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons

Notes

• CD8+ T-cells from HIV-1 CRF02_AG-infected Ivorians could recognize clade B epitopes. No difference was observed in the number of recognized peptide pools between nine French subtype B infected study subjects, and nine CRF02_AG infected Ivorian subjects.
• This epitope was recognized by 0/9 CRF02_AG-infected patients, and by 1/9 B-infected patients. Sequence variants with two amino acid substitutions were found in 5/6 Ivorian subjects.
• An epitope variant was found in 1/4 French patients, and it happened to be the one patient that recognized the epitope: AREmHPEY.

References

Inwoley2005 André Inwoley, Patricia Recordon-Pinson, Marion Dupuis, Jessintha Gaston, Mathieu Genête, Albert Minga, Franck Letourneur, François Rouet, Jeannine Choppin, Hervé Fleury, Jean-Gérard Guillet, Muriel Andrieu, and ANRS 1220 PRIMO-CI Study Group. Cross-Clade Conservation of HIV Type 1 Nef Immunodominant Regions Recognized by CD8+ T Cells of HIV Type 1 CRF02\_AG-Infected Ivorian (West Africa). AIDS Res. Hum. Retroviruses, 21(7):620-628, Jul 2005. PubMed ID: 16060833. Show all entries for this paper.

Displaying record number 54552

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HXB2 Location	Nef(195-202) DNA(9379..9402)	Nef Epitope Map
Author Location	Nef(195-202)
Epitope	ARELHPEY	Epitope Alignment
Epitope Name	AY8
Subtype	B
Species (MHC/HLA)	human(A1)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	escape, acute/early infection, assay or method development, mutation acquisition

Notes

• The ability of T cells from early infected subjects to recognize Nef clade B HIV-1 epitope variants was tested using both a consensus peptide panel and a standardized potential T-cell epitope panel (PTE-B). The magnitude and breadth of PTE-optimized peptide panels was significantly higher than CON peptides'. 29 potential epitopic domains were detected in Nef using the PTE peptide set.
• A PTE-B peptide, FHHkARELHPEYYKD contained the epitope ARELHPEY (AY8) and elicited a IFN-gamma immune response.
• HLA-A01 restriction for AY8 was presumed based on the subject's having the HLA allele and publication in the Los Alamos database.

References

Malhotra2007a Uma Malhotra, Fusheng Li, Jessica Nolin, Megan Allison, Hong Zhao, James I. Mullins, Steve Self, and M. Juliana McElrath. Enhanced Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Nef-Specific T Cells Recognizing Multiple Variants in Early HIV-1 Infection. J. Virol., 81(10):5225-5237, May 2007. PubMed ID: 17329342. Show all entries for this paper.