Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 61768

HXB2 Location	Gag(407-415) p2p7p1p6(44-52) DNA(2008..2034)	Gag Epitope Map
Author Location	p2p7p1p6(44-52)
Epitope	APRKKGCWK	Epitope Alignment
Epitope Name	AK9
Species (MHC/HLA)	human(B*0702)
Immunogen
Experimental methods
Keywords	optimal epitope

Notes

• C. Brander notes this as a B*0702-restricted p2p7p1p6 epitope (Luo2012).

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 61964

HXB2 Location	Gag(407-415) p2p7p1p6(44-52) DNA(2008..2034)	Gag Epitope Map
Author Location	Gag(406-414)
Epitope	APRKKGCWK	Epitope Alignment
Subtype	A, D
Species (MHC/HLA)	human(B*0702)
Immunogen	HIV-1 infection
Patient MHC/HLA	2230: A*0101, A*0301, B*0702, B*5802, Cw*0606, Cw*0702; 1394: A*0201, A*6802, B*0702, B*1302, Cw*0401, Cw*0602
Country	Kenya
Experimental methods	CD8 T-cell Elispot - IFNγ, Other, Tetramer binding
Keywords	HIV exposed persistently seronegative (HEPS), assay or method development

Notes

• A systematic analysis of clade A or D epitopes that bind either protective HLA, A*0101 or susceptible HLA, B*0702 using the iTopia epitope discovery system was conducted. Broad epitope and variant recognition, and immune response was seen with the susceptible HLA allele, while a focused recognition of multipe epitope subtypes was seen associated with the protective HLA allele.
• Novel epitopes were discovered using the iTopia system (3 assays: peptide binding, binding affinity, and off-rate).
• Four new amino acids at anchor positions P2 (Lys, Arg, Thr, Iso) and P9 (Lys, Thr, Arg, Gly) were identified for B*0702 binding, including polar basic residues K and R, showing that B*0702-restricted epitopes are more tolerant to amino acid changes than known previously.
• Previously known B*0702-restricted Gag epitope APRKKGCWK was recognized by seronegative subjects as well as HIV-1 patients and bound HLA-B*0702. 2/8 B*0702+ female subjects responded to the epitope, with a mean of 148 SFU/million cells.

References

Luo2012 Ma Luo, Christina A. Daniuk, Tamsir O. Diallo, Rupert E. Capina, Joshua Kimani, Charles Wachihi, Makubo Kimani, Thomas Bielawny, Trevor Peterson, Mark G. R. Mendoza, Sandra Kiazyk, T. Blake Ball, and Francis A. Plummer. For Protection from HIV-1 Infection, More Might Not Be Better: A Systematic Analysis of HIV Gag Epitopes of Two Alleles Associated with Different Outcomes of HIV-1 Infection. J. Virol., 86(2):1166-1180, Jan 2012. PubMed ID: 22072744. Show all entries for this paper.