Search CTL/CD8+ T-Cell Epitope Database

Found 25 matching records:

Displaying record number 53720

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag(197-205 BRU)
Epitope	`AMQMLKETI`	Epitope Alignment
Subtype	B, CRF02_AG
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Country	Cote D'Ivoire
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons

Notes

CD8+ T-cells from HIV-1 CRF02_AG-infected Ivorians could recognize clade B epitopes. No difference was observed in the number of recognized peptide pools between nine French subtype B infected study subjects, and nine CRF02_AG infected Ivorian subjects.
This epitope was recognized by 1/9 CRF02_AG-infected Ivorians, and 1/9 B-infected French subjects.

References

Inwoley2005 André Inwoley, Patricia Recordon-Pinson, Marion Dupuis, Jessintha Gaston, Mathieu Genête, Albert Minga, Franck Letourneur, François Rouet, Jeannine Choppin, Hervé Fleury, Jean-Gérard Guillet, Muriel Andrieu, and ANRS 1220 PRIMO-CI Study Group. Cross-Clade Conservation of HIV Type 1 Nef Immunodominant Regions Recognized by CD8+ T Cells of HIV Type 1 CRF02\_AG-Infected Ivorian (West Africa). AIDS Res. Hum. Retroviruses, 21(7):620-628, Jul 2005. PubMed ID: 16060833. Show all entries for this paper.

Displaying record number 52912

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	p24(199-207 SF2)
Epitope	`AMQMLKETI`	Epitope Alignment
Epitope Name	p24(199-207)
Subtype	B
Species (MHC/HLA)	mouse(H-2D^d)
Immunogen	vaccine
Experimental methods	Chromium-release assay
Keywords	epitope processing, vaccine-induced immune responses

Vaccine Details

Vaccine type	DNA with CMV promotor
Vaccine strain	B clade SF2
Vaccine component	Gag, gp120

Notes

Spleen cells from mice with distinct MHC types were infused into HIV vaccinated scid mice, to study the antigen presenting cells used by CTL induced in intramuscular injections. Bone marrow derived cells are used for presentation, but DNA infection is not required for priming, rather APCs can present proteins synthesized in other host cells.

References

Doe1996a Barbara Doe, Mark Selby, Susan Barnett, Juerg Baenziger, and Christopher M. Walker. Induction of Cytotoxic T Lymphocytes by Intramuscular Immunization with Plasmid DNA Is Facilitated by Bone Marrow-Derived Cells. Proc. Natl. Acad. Sci. U.S.A., 93(16):8578-8583, 6 Aug 1996. PubMed ID: 8710913. Show all entries for this paper.

Displaying record number 207

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	p24(199-207 SF2)
Epitope	`AMQMLKETI`	Epitope Alignment
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods
Keywords	immunodominance

Vaccine Details

Vaccine type	vaccinia
Vaccine component	Gag, Pol

Notes

Immunodominant murine CTL response to this peptide observed after immunization with vaccine VVgagpol.
Optimal peptide was defined.

References

Doe1996 B Doe and C M Walker. HIV-1 p24 Gag-Specific Cytotoxic T-Lymphocyte Responses in Mice. AIDS, 10(7):793-4, Jun 1996. PubMed ID: 8805872. Show all entries for this paper.

Displaying record number 1515

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag(197-205 SF2)
Epitope	`AMQMLKETI`	Epitope Alignment
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods
Keywords	immunodominance

Vaccine Details

Vaccine type	Listeria monocytogenes
Vaccine strain	B clade HXB2
Vaccine component	Gag

Notes

BALB/c mice were immunized with recombinant Listeria monocytogenes (Lm-Gag) expressing HIV-1 HXB2 Gag.
L. monocytogenes is a gram-positive bacteria that enters the macrophage on phagocytosis and lives in the cytoplasm -- secreted L. monocytogenes antigens are processed and presented by both class I and class II pathways.
This is the immunodominant CTL epitope in Gag in BALB/c mice.
AMQMLKETI does not contain established Kd anchoring residue in position 2, tyrosine or phenylalanine, thus deviating from the typical Kd anchoring motif -- the lack of the aromatic anchor residue is compensated for by interaction of the glutamine at P3 with pocket D of Kd.

References

Mata1998 M. Mata, P. J. Travers, Q. Liu, F. R. Frankel, and Y. Paterson. The MHC Class I-Restricted Immune Response to HIV-gag in BALB/c Mice Selects a Single Epitope That Does Not Have a Predictable MHC-Binding Motif and Binds to Kd through Interactions between a Glutamine at P3 and Pocket D. J. Immunol., 161:2985-2993, 1998. PubMed ID: 9743362. Show all entries for this paper.

Displaying record number 52190

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag( HXB2)
Epitope	`AMQMLKETI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods
Keywords	immunodominance

Vaccine Details

Vaccine type	vaccinia, vesicular stomatitis virus (VSV)
Vaccine strain	B clade HXB2, B clade IIIB
Vaccine component	Env, Gag

Notes

Different HIV strains were used for different regions: Env IIIB, Gag HXB2.
BALB/c mice were vaccinated with rec vesicular stomatitis virus (rVSV) expressing either HIV-1 Gag, Env, or both, and compared to using rec Env and Gag in vaccinia virus (rVVs). The primary response was determined by cell lysis, cytokine production and tetramer staining.
Primary CTL responses to the immunodominant Gag (AMQMLKETI) epitope peaked in 7 days for GAG-rVSV, 3% of the cells were tetramer positive, and this response was 8-fold higher than for Gag-rVV.
Vaccinating with GagEnv-rVSV carrying both Gag and Env allowed recognition of both HIV-1 proteins, but at reduced levels compared to either Gag-rVSV or Env-rVSV alone.
Intranasal immunization with Env-rVSV yielded CTL responses that were strong but reduced compared to an intraperitoneal route.

References

Haglund2002a Karl Haglund, Ingrid Leiner, Kristen Kerksiek, Linda Buonocore, Eric Pamer, and John K. Rose. High-Level Primary CD8(+) T-Cell Response to Human Immunodeficiency Virus Type 1 Gag and Env Generated by Vaccination with Recombinant Vesicular Stomatitis Viruses. J. Virol., 76(6):2730-2738, Mar 2002. PubMed ID: 11861840. Show all entries for this paper.

Displaying record number 53145

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location
Epitope	`AMQMLKETI`	Epitope Alignment
Epitope Name	A9I
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods	Chromium-release assay, Cytokine production
Keywords	epitope processing, vaccine-induced immune responses, immunodominance, vaccine antigen design

Vaccine Details

Vaccine type	DNA, virus-like particle (VLP), polyepitope
Vaccine component	Gag, p24 Gag, Env V3

Notes

A codon optimized gag DNA vaccine was compared to a myristylation defective gag and p24 alone, both of which lack signals for secretion from transfected cells. Gag-derived immunogens that were secreted as VLPs and those that remained intracellular (p24) each produced strong CTL responses, and neither the size of antigen nor cellular trafficking and localization significantly influenced the strength of humoral and cellular immune activation. The formation and release of VLPs was not essential for eliciting strong CTL. BALB/c mice were given the DNA vaccine by i.m. administration of plasmid DNA for the prime and boost.
Linking the region encoding the V3 immunodominant epitope to the gag gene did not diminish the response to the Gag p24 epitope A9I, but did enable a response to the V3 epitope.
Minigenes were made incorporating just 1 epitope, minitopes, carrying 1 of 3 murine class I epitopes linked to the Ad2-E3 protein-derived signal peptide to allow access of the epitope to the ER. Weak induction of cellular immune responses was observed, in contrast to the complex polyprotein.

References

Wild2004 Jens Wild, Alexandra Bojak, Ludwig Deml, and Ralf Wagner. Influence of Polypeptide Size and Intracellular Sorting on the Induction of Epitope-Specific CTL Responses by DNA Vaccines in a Mouse Model. Vaccine, 22(13-14):1732-1743, 15 Apr 2004. PubMed ID: 15068857. Show all entries for this paper.

Displaying record number 1391

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag(197-205)
Epitope	`AMQMLKETI`	Epitope Alignment
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods
Keywords

Vaccine Details

Vaccine type	Listeria monocytogenes
Vaccine component	Gag

Notes

BALB/c mice were immunized with a highly attenuated recombinant Listeria monocytogenes, Lmdaldat, that can grow only when supplemented with D-alanine, and that expresses HIV-1 HXB2 Gag.
Parenteral immunization provided protection against systemic and mucosal challenges with a recombinant vaccinia virus expressing HIV-1 gag, and a long lasting memory CTL response against Gag in spleen, mesenteric lymph nodes, and Peyer's patches directed against the gag protein.
Oral immunization gave protection only against mucosal virus challenge and was associated with a transient CTL response in the three lymphoid tissues examined.
L. monocytogenes is a gram-positive bacteria that enters the macrophage on phagocytosis and lives in the cytoplasm -- secreted L. monocytogenes antigens are processed and presented by both class I and class II pathways.

References

Rayevskaya2001 M. V. Rayevskaya and F. R. Frankel. Systemic immunity and mucosal immunity are induced against human immunodeficiency virus Gag protein in mice by a new hyperattenuated strain of Listeria monocytogenes. J. Virol., 75(6):2786-91, Mar 2001. URL: http://jvi.asm.org/cgi/content/full/75/6/2786. PubMed ID: 11222702. Show all entries for this paper.

Displaying record number 59152

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag(65-73)
Epitope	`AMQMLKETI`	Epitope Alignment
Epitope Name	Gag-AI9
Subtype	B
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Tetramer binding
Keywords	characterizing CD8+ T cells

Vaccine Details

Vaccine type	AIDSVAX B/E
Vaccine component	Gag-Pol

Notes

Antigen dosage effects (10⁸ or 10¹⁰) on immune profile were examined across tissues using an Ad5.Gag.Pol HIV-1 vaccine in BALB/c mice. Two immunodominant CTL populations that recognize Gag-AI9 and Pol-LI9 were characterized and had similar clonality, but Gag-AI9 clones elicited higher avidity and lysis. Lower dose vaccination enhanced functional profiles and proportions of central memory cells.
Gag-AI9-specific CTL were more robust after high-dose vaccination, and retained their clonotypic diversity. They lacked any preference for TRBV/TRBJ usage or CDR3β sequence motif. These cells contained large numbers of nucleotide additions at TCR junctional regions.

References

Hill2014 Brenna J. Hill, Patricia A. Darrah, Zachary Ende, David R. Ambrozak, Kylie M. Quinn, Sam Darko, Emma Gostick, Linda Wooldridge, Hugo A. van den Berg, Vanessa Venturi, Martin Larsen, Miles P. Davenport, Robert A. Seder, David A. Price, and Daniel C. Douek. Epitope Specificity Delimits the Functional Capabilities of Vaccine-Induced CD8 T Cell Populations. J. Immunol., 193(11):5626-5636, 1 Dec 2014. PubMed ID: 25348625. Show all entries for this paper.

Displaying record number 52771

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag
Epitope	`AMQMLKETI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Patient MHC/HLA	H-2d
Experimental methods	Intracellular cytokine staining, Tetramer binding
Keywords	genital and mucosal immunity

Vaccine Details

Vaccine type	Listeria monocytogenes
Vaccine component	Gag

Notes

Intravenous, rectal, and oral vaccination of recombinant L. monocytogenes expressing HIV-1 Gag antigen were compared for their ability to stimulate a mucosal CTL response; mucosal administration of this vaccine gave strong mucosal response that was readily boosted.
This CTL epitope is the immunodominant epitope in Gag for BALB/c mice, and was used to characterize the vaccine responses.

References

Peters2003 Christian Peters, Xiaohui Peng, Dennis Douven, Zhen-Kun Pan, and Yvonne Paterson. The Induction of HIV Gag-Specific CD8+ T Cells in the Spleen and Gut-Associated Lymphoid Tissue by Parenteral or Mucosal Immunization with Recombinant Listeria monocytogenes HIV Gag. J. Immunol., 170(10):5176-5187, May 2003. PubMed ID: 12734365. Show all entries for this paper.

Displaying record number 52773

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag(197-205)
Epitope	`AMQMLKETI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Patient MHC/HLA	H-2d
Experimental methods	Chromium-release assay, Cytokine production, Intracellular cytokine staining, Proliferation, Tetramer binding
Keywords	memory cells

Vaccine Details

Vaccine type	vaccinia, Listeria monocytogenes
Vaccine component	Gag, Nef

Notes

Splenocytes derived from BALB/c mice immunized and boosted with Lmdd-gag were stimulated with gag-peptide specific antigen in vitro. In culture, CTL activity against this epitope reached a maximum at 9 days, then declined. Peptide restimulation gave a delayed (18 hours) but potent response, and growth was IL-2 or IL-15 dependent. Adoptive transfer of 5000 of the sorting purified cells could protect recipient BALB/c against vaccinia-gag challenge up to 3 months after transfer.

References

Rayevskaya2003 Marina Rayevskaya, Natasha Kushnir, and Fred R. Frankel. Anti-Human Immunodeficiency Virus-Gag CD8+ Memory T Cells Generated In Vitro From Listeria-Immunized Mice. Immunology, 109(3):450-460, Jul 2003. PubMed ID: 12807492. Show all entries for this paper.

Displaying record number 57180

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag(197-205)
Epitope	`AMQMLKETI`	Epitope Alignment
Epitope Name	Gag197-205
Subtype	CRF01_AE
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other, Tetramer binding
Keywords	vaccine-induced immune responses, characterizing CD8+ T cells

Vaccine Details

Vaccine type	fowlpox prime with modified vaccinia Ankara (MVA) boost
Vaccine strain	CRF01_AE clade
Vaccine component	Env, Gag, Pol, Rev, Tat

Notes

Influence of IL-4 and IL-13 expression on K(d)Gag197-205-specific CTL avidity was studied using mucosal or systemic methods of HIV-1 vaccine delivery. A number of mouse KO models were used to determine that while mucosal immunization elicits CTL of higher avidity, systemic immunization produces higher IL-4 and IL-13. CTL avidity and CCL5 expression are inversely related to IL-4/IL-13 expression. IL-4 expression, however, helps maintain Th1/Th2 cytokine balance in the cell milieu.
CTL specific to Gag peptide epitope AMQMLKETI was assessed in these studies.

References

Ranasinghe2009 Charani Ranasinghe and Ian A. Ramshaw. Immunisation Route-Dependent Expression of IL-4/IL-13 Can Modulate HIV-Specific CD8+ CTL Avidity. Eur. J. Immunol., 39(7):1819-1830, Jul 2009. PubMed ID: 19582753. Show all entries for this paper.

Displaying record number 52210

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag( HXB2)
Epitope	`AMQMLKETI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods
Keywords	immunodominance

Vaccine Details

Vaccine type	vaccinia, vesicular stomatitis virus (VSV)
Vaccine strain	B clade HXB2, B clade IIIB
Vaccine component	Env, Gag

Notes

Different HIV strains were used for different regions: Env IIIB, Gag HXB2.
BALB/c mice were vaccinated with rec vesicular stomatitis virus (rVSV) expressing either HIV-1 Gag or Env, or both, and retention of memory responses and recall responses were studied by tetramer staining and IFN-gamma production.
Seven months after vaccination with Env-rVSV, 6% of the CD8+ cells were tetramer positive for the immunodominant Env epitope; these cells had a memory phenotype, CD44-Hi positive.
Env in rec vaccinia virus (Env-rVV) elicited a strong recall response, with up to 45% to the CD8+ T-cell population tetramer positive and activated (expressing CD62L-Lo), and capable of IFN-gamma production.
A prime with Env-rVSV and heterologous boost of Env-rVV gave remarkably high levels of memory cells, with approximately 1/3 of the CD8+ splenocytes being Env specific memory cells 150 days after the boost.
A Gag-rVSV or EnvGag-rVSV prime and with a heterologous Gag-rVV or EnvGag-rVV boost combination gave 40% tetramer positive CD8+ cells, but the fraction of IFN-gamma producing cells was only about 25%. Still the heterologous vector prime-boost combination showed a profound benefit.
A HIV-1 protein rVSV prime, rVV boost was a more potent combination than a vector reversal of a rVV prime and rVSV boost.

References

Haglund2002b Karl Haglund, Ingrid Leiner, Kristen Kerksiek, Linda Buonocore, Eric Pamer, and John K. Rose. Robust Recall and Long-Term Memory T-Cell Responses Induced by Prime-Boost Regimens with Heterologous Live Viral Vectors Expressing Human Immunodeficiency Virus Type 1 Gag and Env Proteins. J. Virol., 76(15):7506-7517, Aug 2002. PubMed ID: 12097563. Show all entries for this paper.

Displaying record number 53274

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag(197-205)
Epitope	`AMQMLKETI`	Epitope Alignment
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Country	United States
Experimental methods	Proliferation, T-cell Elispot
Keywords	vaccine antigen design

Vaccine Details

Vaccine type	vaccinia
Vaccine strain	B clade HXB2
Vaccine component	Gag

Notes

A recombinant vaccinia virus with HIV-1 Gag replacing the cytoplasmic domain of the B5R protein was shown to induce better primary CD4 response than recombinant vaccinia virus expressing Gag from the TK-locus; CD8 responses were less specific. When immunized BALB/c mice were challenged with a recombinant Listeria that expresses HIV-Gag, lower colony counts of Listeria were found in the liver and spleen of mice immunized with virus expressing B5R-Gag fusion protein.

References

Kwak2004 Heesun Kwak, Waleed Mustafa, Kendra Speirs, Asha J. Abdool, Yvonne Paterson, and Stuart N. Isaacs. Improved Protection Conferred by Vaccination with a Recombinant Vaccinia Virus That Incorporates a Foreign Antigen into the Extracellular Enveloped Virion. Virology, 322(2):337-348, 1 May 2004. PubMed ID: 15110531. Show all entries for this paper.

Displaying record number 206

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag(199-207 HXB2)
Epitope	`AMQMLKETI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse(H-2^d)
Immunogen	vaccine
Experimental methods
Keywords

Vaccine Details

Vaccine type	DNA
Vaccine strain	B clade HXB2
Vaccine component	Gag

Notes

Different expression vectors were tested to increase Gag expression in cell lines and create suitable vectors for DNA vaccines.
Stable Gag expression was achieved in murine p815 cells, using a Gag gene that had mutated silent base positions that disrupt inhibitory RNA sequences which promote RNA degradation.
Silent mutations were more effective than introduction of the D retrovirus cis-acting posttranscriptional control element (CTE) for enhancing Gag expression.
The gag vector with silent mutations given as a vaccine to BALB/c mice gave CTL responses in splenic mononuclear cells, using peptide pulsed cells as targets.

References

Qiu1999 J. T. Qiu, R. Song, M. Dettenhofer, C. Tian, T. August, B. K. Felber, G. N. Pavlakis, and X. F. Yu. Evaluation of Novel Human Immunodeficiency Virus Type 1 Gag DNA Vaccines for Protein Expression in Mammalian Cells and Induction of Immune Responses. J. Virol., 73:9145-9152, 1999. PubMed ID: 10516021. Show all entries for this paper.

Displaying record number 1399

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	p24(199-207 SF2)
Epitope	`AMQMLKETI`	Epitope Alignment
Epitope Name	p7g
Species (MHC/HLA)	mouse(H-2^d)
Immunogen	vaccine
Experimental methods
Keywords

Vaccine Details

Vaccine type	protein, vaccinia
Vaccine strain	B clade SF2
Vaccine component	Gag, Gag-Pol
Adjuvant	E. coli mutant heat labile enterotoxin (LT-R72)

Notes

Intranasal immunization of CB6F1 (H2bxd) mice with soluble gag p55 with LT ADP-ribosyltransferase mutants (LTK63 and LTK73) from Escherichia coli as adjuvants was tested.
Intranasal and intramucosal immunization of p55 gag protein with LTK63 or LTK72 adjuvant induced a CTL response comparable to intramuscular immunization responses.
Oral co-administration of LTR72, with residual ADP-ribosyltransferase activity, induced systemic CTL responses, but LTK63 with no ADP-ribosyltransferase activity did not.

References

Neidleman2000 J. A. Neidleman, M. Vajdy, M. Ugozzoli, G. Ott, and D. O'Hagan. Genetically detoxified mutants of heat-labile enterotoxin from Escherichia coli are effective adjuvants for induction of cytotoxic T-cell responses against HIV-1 gag-p55. Immunology, 101(1):154-60, Sep 2000. PubMed ID: 11012767. Show all entries for this paper.

Displaying record number 52302

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	p24(66-74)
Epitope	`AMQMLKETI`	Epitope Alignment
Species (MHC/HLA)	mouse(H-2^d)
Immunogen	vaccine
Experimental methods
Keywords

Vaccine Details

Vaccine type	DNA
Vaccine component	Gag
Adjuvant	vesicular stomatitis virus glycoprotein (VSV-G)

Notes

BALB/c mice were injected with plasmids expressing HIV-1 Gag with or without coinjection of a plasmid expressing vesicular stomatitis virus glycoprotein (VSV-G). The combination encodes VSV-G pseudotyped Gag particles that can be taken up by cells for presentation in either the class I or class II pathways, while exogenous Gag alone can only be taken into the class II pathway.
Vaccination with DNA expressing VSV-G pseudotyped Gag particles rather than just Gag increase Gag-specific CTL responses generally as well as the specific H-2d restricted anti-AMQMLKETI response.

References

Marsac2002 D. Marsac, D. Loirat, C. Petit, O. Schwartz, and M.-L. Michel. Enhanced Presentation of Major Histocompatibility Complex Class I-Restricted Human Immunodeficiency Virus Type 1 (HIV-1) Gag-Specific Epitopes after DNA Immunization with Vectors Coding for Vesicular Stomatitis Virus Glycoprotein-Pseudotyped HIV-1 Gag Particles. J. Virol., 76(15):7544-53, Aug 2002. PubMed ID: 12097567. Show all entries for this paper.

Displaying record number 55113

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag
Epitope	`AMQMLKETI`	Epitope Alignment
Subtype	A, B
Species (MHC/HLA)	mouse(H-2^d)
Immunogen	vaccine
Country	Finland
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	vaccine antigen design

Vaccine Details

Vaccine type	DNA
Vaccine strain	A clade, B clade
Vaccine component	Env, Gag

Notes

A novel mouse model was used to test the efficacy of 2 HIV DNA vaccines in protection against tumor challenge. Comparable immunogenicity between the single and multi-clade vaccines tested was seen in different mouse strains. CTL response to HIV-1-APCs was both in vivo and in vitro and this animal model not only evaluated vaccine immunogenicity but also confirmed the potency of GTU-multi-HIV vaccines.

References

Malm2007 Maria Malm, Rein Sikut, Kai Krohn, and Vesna Blazevic. GTU-MultiHIV DNA Vaccine Results in Protection in a Novel P815 Tumor Challenge Model. Vaccine, 25(17):3293-301, 30 Apr 2007. PubMed ID: 17289222. Show all entries for this paper.

Displaying record number 59103

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	p24(65-73)
Epitope	`AMQMLKETI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human, mouse(H-2^d)
Immunogen	vaccine
Experimental methods	CD4 T-cell Elispot - IFNγ, CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	DNA
Vaccine strain	A clade, B clade, C clade
Vaccine component	Env, Gag, Nef, Pol, Tat

Notes

An engineered artificial polyepitope (polyE) vaccine consisting 50 CTL epitopes across Env, Gag, Pol, Nef and Tat, 7 helper epitopes, additional signal sequences and selected to cover the most of 35 HLA was delivered to BALB/c mice over a triple immunization schedule. Splenocytes from vaccinated mice were challenged by 6 CTL epitopes to determine magnitude of response.
Vaccine epitopes were selected from the list at HIV Immunology database according to conservation across clades A, B, C. PolyE was linked to N-terminal ubiquitin (proteasomal trageting, MHC I loading), N-signal peptide (ER and secretory pathway delivery) and/or C-terminal LAMP-1 protein (directs immunogen from secretory pathway to lysosomes, MHC II loading) to give 3 constructs: TCI-N (polyE), TCI-N2 (ER signal_polyE_LAMP-1), and TCI-N3 (Ubi_polyE). All groups of mice developed both CTL and helper T-cell responses, but N-terminal ubiquitination mediated the highest expression of IL-2 and IFN-γ.
Conserved HIV-1 epitope AMQMLKETI restricted by mouse BALB/c HLA-H-2^d was included in the vaccine and elicited T-cell responses.

References

Reguzova2015 Alena Reguzova, Denis Antonets, Larisa Karpenko, Alexander Ilyichev, Rinat Maksyutov, and Sergei Bazhan. Design and Evaluation of Optimized Artificial HIV-1 Poly-T Cell-Epitope Immunogens. PLoS One, 10(3):e0116412, 2015. PubMed ID: 25786238. Show all entries for this paper.

Displaying record number 58685

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag
Epitope	`AMQMLKETI`	Epitope Alignment
Species (MHC/HLA)	mouse(H-2^kd)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	adenovirus type 5 (Ad5)
Vaccine component	Gag

Notes

Since recombinant adenovirus serotype 5 (rAd5) based vaccines are thought to fail frequently because of pre-existing immunity to Ad5, the authors experiment with an EAT-2-expressing rAd5 vector. EAT-2 is a member of the signaling lymphocyte activation molecule (SLAM) family of receptor adaptors that is costimulatory, initiating signal-transduction networks in both innate and adaptive immunities.
Using Gag immunodominant epitope, AMQMLKETI, in vaccinated mice, it was shown by EliSpot and tetramer staining that cellular immunity is heightened with rad5-EAT-2 immunization. Boosting immunizations elicited greater long-term T cell memory responses, CD4+ responses; and no fall in humoral immunity. This was not seen in a mutant rAd5-EAT-2(R31Q) vaccine. Studies were conducted in Ad5-immune mice.

References

Aldhamen2012 Yasser Ali Aldhamen, Sergey S. Seregin, Nathaniel J. Schuldt, David P. W. Rastall, Chyong-jy J. Liu, Sarah Godbehere, and Andrea Amalfitano. Vaccines Expressing the Innate Immune Modulator EAT-2 Elicit Potent Effector Memory T Lymphocyte Responses Despite Pre-Existing Vaccine Immunity. J. Immunol., 189(3):1349-1359, 1 Aug 2012. PubMed ID: 22745373. Show all entries for this paper.

Displaying record number 52310

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	p24(199-207 SF2)
Epitope	`AMQMLKETI`	Epitope Alignment
Epitope Name	p7G
Species (MHC/HLA)	mouse(H-2^kd)
Immunogen	vaccine
Experimental methods
Keywords	dendritic cells

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade SF2
Vaccine component	Gag
Adjuvant	DDA, DOTAP, CpG immunostimulatory sequence (ISS), MF59, PLG, urea

Notes

Intramuscular or intraperitoneal immunization of BALB/c or CB6F1 mice with urea-solubilized, emulsified, or PLG-microparticle associated p55 Gag was studied in conjunction with the adjuvant CpG. CpG did not enhance CTL immunity when combined with urea solubilized p55, but did when combined with emulsions and PLG-microparticle antigen.
CpG shifted the Ab response towards a IgG2a, and CpG was shown to upregulate CD86 on mouse bone-marrow derived dendritic cells.

References

OHagan2002 D. T. O'Hagan, M. Singh, J. Kazzaz, M. Ugozzoli, M. Briones, J. Donnelly, and G. Ott. Synergistic Adjuvant Activity of Immunostimulatory DNA and Oil/Water Emulsions for Immunization with HIV p55 Gag Antigen. Vaccine, 20(27-28):3389-3398, 10 Sep 2002. PubMed ID: 12213409. Show all entries for this paper.

Displaying record number 52783

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag(199-207)
Epitope	`AMQMLKETI`	Epitope Alignment
Epitope Name	p7g
Subtype	B
Species (MHC/HLA)	mouse
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	genital and mucosal immunity

Vaccine Details

Vaccine type	vaccinia, Sindbis
Vaccine component	Gag

Notes

Nasal, vaginal, rectal and i.m. immunization was performed with Sindbis virus expressing HIV-1 Gag (SIN-Gag), followed by intravaginal or intrarectal challenge with vaccinia virus expressing either Gag (VV-Gag) or gp160 (VV-gp160) as a control.
Intranasal and intramuscular immunization followed by intravaginal challenge induced HIV-1 Gag specific, IFN-γ producing CD8+ T-cells in the vaginal/uteral mucosal tissue, as well as in the draining iliac lymph nodes and in the spleen, but could not protect against a VV-Gag infection of the ovaries. Local vaginal or rectal immunization, despite lower CD8+ T-cell responses, did provide protection.

References

Vajdy2001 Michael Vajdy, Jason Gardner, Jason Neidleman, Lina Cuadra, Catherine Greer, Silvia Perri, Derek O'Hagan, and John M. Polo. Human Immunodeficiency Virus Type 1 Gag-Specific Vaginal Immunity and Protection after Local Immunizations with Sindbis Virus-Based Replicon Particles. J. Infect. Dis., 184(12):1613-1616, Dec 2001. PubMed ID: 11740739. Show all entries for this paper.

Displaying record number 61137

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location
Epitope	`AMQMLKETI`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human, mouse
Immunogen	HIV-1 infection, vaccine
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	DNA, modified vaccinia Ankara (MVA), adenovirus
Vaccine strain	B clade

Notes

Using 3 strategies, (1) in silico-designed multivalent-mosaic proteins for greater breadth, magnitude, cross-reactivity (2) highly conserved epitopes for loss of replicative fitness and oligofunctional inter-clade specific T-cell production (3) beneficial CTL epitopes associated with low viral load (VL) for broad immune response; a conserved bivalent-mosaic immunogen, tHIV-consvX, was designed and vectored by plasmid DNA, ChAdOx1, and MVA for testing in mice and humans. Strong preclinical immunogenicity was seen in mice and in 120 untreated, chronic HIV-1 patients, tHIVconsvX-specific CTLs correlated with high CD4+ T cell count but not VL.
Env was excluded from this second generation vaccine as its short, conserved stretch has no beneficial epitopes and while its epitopes are immunodominant, they are not associated with good control of viremia.
Epitope AMQMLKETI was present in mosaic 2, being immunodominant in response and eliciting a stronger response than its variant AMQMLKDTI in Mosaic 1.

References

Ondondo2016 Beatrice Ondondo, Hayato Murakoshi, Genevieve Clutton, Sultan Abdul-Jawad, Edmund G.-T. Wee, Hiroyuki Gatanaga, Shinichi Oka, Andrew J. McMichael, Masafumi Takiguchi, Bette Korber, and Tomáš Hanke. Novel Conserved-region T-cell Mosaic Vaccine With High Global HIV-1 Coverage Is Recognized by Protective Responses in Untreated Infection. Mol. Ther., 24(4):832-842, Apr 2016. PubMed ID: 26743582. Show all entries for this paper.

Displaying record number 55739

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag
Epitope	`AMQMLKETI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	vaccine
Experimental methods	Other
Keywords	vaccine antigen design

Vaccine Details

Vaccine type	modified vaccinia Ankara (MVA)
Vaccine strain	B clade ADA
Vaccine component	Env, Gag-Pol

Notes

An in-frame initiation codon upsteam of Env gene in rMVA increases Env protein 5-fold in inoculated mice. This results in enhanced immune responses to Env that do not affect responses to Gag.
The immunodominant Gag peptide AMQMLKETI was used to elicit IFN-gamma production as a marker of CTL function against Gag.

References

Wyatt2008 Linda S. Wyatt, Patricia L. Earl, Jennifer Vogt, Leigh Anne Eller, Dev Chandran, Jinyan Liu, Harriet L. Robinson, and Bernard Moss. Correlation of Immunogenicities and In Vitro Expression Levels of Recombinant Modified Vaccinia Virus Ankara HIV Vaccines. Vaccine, 26(4):486-493, 24 Jan 2008. PubMed ID: 18155813. Show all entries for this paper.

Displaying record number 61470

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag
Epitope	`AMQMLKETI`	Epitope Alignment
Species (MHC/HLA)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses

Vaccine Details

Vaccine type	adenovirus
Vaccine component	Gag

Notes

Specific targeting of CRACC (CD2-like receptor activating cytotoxic cell) functions during vaccination by overexpression of adaptor protein EAT-2 (Ewing's sarcoma-associated transcript 2) potently enhances Natural Killer (NK) activation and Dendritic Cell (DC) maturation which are innate responses and improves CTL response to the vaccine-antigen, Gag in mice.
AMQMLKETI was the Gag epitope used to measure CTL response by EliSpot.

References

Aldhamen2016 Yasser A. Aldhamen, David P. W. Rastall, Weimin Chen, Sergey S. Seregin, Cristiane Pereira-Hicks, Sarah Godbehere, Norbert E. Kaminski, and Andrea Amalfitano. CRACC-Targeting Fc-Fusion Protein Induces Activation of NK Cells and DCs and Improves T Cell Immune Responses to Antigenic Targets. Vaccine, 34(27):3109-3118, 8 Jun 2016. PubMed ID: 27151882. Show all entries for this paper.

Displaying record number 57721

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag( HIV 89.6)
Epitope	`AMQMLKETI`	Epitope Alignment
Species (MHC/HLA)	mouse
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine antigen design

Notes

Normally immunization with HIV Env DNA induces a strong CTL response but weak antibody response, while virus-like particle (VLP) vaccines induce a stronger antibody than CTL response. Here a mixtures of Env DNA and VLP vaccines is used to combine the advantages of both.
HIV-1 Gag epitope AMQMLKETI was used as a negative control peptide to test immunity post-immunization with Env.

References

Ye2010 Ling Ye, Zhiyuan Wen, Ke Dong, Lei Pan, Zhigao Bu, Richard W. Compans, Huizhong Zhang, and Chinglai Yang. Immunization with a Mixture of HIV Env DNA and VLP Vaccines Augments Induction of CD8 T Cell Responses. J. Biomed. Biotechnol., 2010:497219, 2010. PubMed ID: 20508832. Show all entries for this paper.