Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 52588

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HXB2 Location	Gag(197-204) p24(65-72) DNA(1378..1401)	Gag Epitope Map
Author Location	p24
Epitope	AMQMLKET	Epitope Alignment
Epitope Name	A9I
Species (MHC/HLA)	mouse(H-2d)
Immunogen	vaccine
Experimental methods	Chromium-release assay
Keywords

Vaccine Details

Notes

• Codon-optimized gag gene DNA vaccines were compared to wild type by vaccination of BALB/c mice. Codon optimized DNA gave a Th1 polarized Th response, a strong antibody response that persisted from more than 20 weeks, and CTL responses, while wild-type DNA induced weak and inconsistent immune responses.

References

Bojak2002a Alexandra Bojak, Jens Wild, Ludwig Deml, and Ralf Wagner. Impact of Codon Usage Modification on T Cell Immunogenicity and Longevity of HIV-1 Gag-Specific DNA Vaccines. Intervirology, 45(4-6):275-86, 2002. PubMed ID: 12566710. Show all entries for this paper.

Displaying record number 56241

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HXB2 Location	Gag(197-204) p24(65-72) DNA(1378..1401)	Gag Epitope Map
Author Location	Gag(197-205 SF2)
Epitope	AMQMLKET	Epitope Alignment
Epitope Name	AMQ
Subtype	B
Species (MHC/HLA)	mouse(H-2d)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	vaccine-induced immune responses

Vaccine Details

Notes

• In order to increase the known number of epitopes in Gag protein so that they can be assayed for reactivity in vaccine studies, BALB/c mice were inoculated with Gag or Gag+Tat. OLPs were used to elicit T-cell immune responses from mouse splenocytes, tested for by cytotoxicity or ELISpot assays. 5 novel predicted optimal CTL epitopes, 2 novel predicted CD4 epitopes and 2 immune response eliciting peptides were found.
• Previously known epitope AMQMLKET was found in reactive Peptides 49 and 50, GHQAAMQMLKETINEEAAE and AMQMLKETINEEAAE.

References

Cellini2008 Silvia Cellini, Cinzia Fortini, Eleonora Gallerani, Federica Destro, Egidio Brocca Cofano, Antonella Caputo, and Riccardo Gavioli. Identification of New HIV-1 Gag-Specific Cytotoxic T Lymphocyte Responses in BALB/c Mice. Virol. J., 5:81, 2008. PubMed ID: 18625037. Show all entries for this paper.