Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 1396

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HXB2 Location	Nef(42-50) DNA(8920..8946)	Nef Epitope Map
Author Location	Nef(44-52 HXB3)
Epitope	ALTSSNTAA	Epitope Alignment
Species (MHC/HLA)	mouse(A*02:01)
Immunogen	vaccine
Experimental methods
Keywords	binding affinity, computational epitope prediction

Vaccine Details

Notes

• Ten Nef 9-mer peptides were predicted to have a strong binding affinity with HLA-A*0201 -- of these, four did bind strongly by a T2 class I stabilization assay, several others bound weakly.
• A CTL immune response to only 3/10 peptides was detected by a 51Cr-release assay after immunization of HLA-A201 transgenic mice with either nef DNA under the control of a CMV promotor, coated on gold particles delivered to abdominal skin by gene gun.
• ALTSSNTAA was also tested by subcutaneous injection of Nef peptides in Freund's adjuvant.
• ALTSSNTAA bound weakly to HLA-A2, but it had the strongest CTL response among the three elicited by the DNA vaccine and a strong response to the peptide vaccination.

References

Sandberg2000 J. K. Sandberg, A. C. Leandersson, C. Devito, B. Kohleisen, V. Erfle, A. Achour, M. Levi, S. Schwartz, K. Karre, B. Wahren, and J. Hinkula. Human immunodeficiency virus type 1 Nef epitopes recognized in HLA-A2 transgenic mice in response to DNA and peptide immunization. Virology, 273(1):112-9, 20 Jul 2000. PubMed ID: 10891413. Show all entries for this paper.

Displaying record number 53175

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HXB2 Location	Nef(42-50) DNA(8920..8946)	Nef Epitope Map
Author Location	Nef(42-50)
Epitope	ALTSSNTAA	Epitope Alignment
Epitope Name	Nef42-50
Species (MHC/HLA)	human, humanized mouse(A2)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	responses in children, immunodominance, characterizing CD8+ T cells

Notes

• Responses to five HLA-A2 presented epitopes in Nef were characterized in a population of 19 HIV infected adults and 21 children. The CD8 T-cell response to Nef was stronger and broader in adults than children, averaging 652 and 87 SFCs/10⁶ PBMC, and 2.6 and 0.9 recognized epitopes per person, respectively.
• This is one of three novel Nef epitopes previously identified in HLA-A2 transgenic mice, shown to induce CD8 T-cell response in humans. It was not the immunodominant response.

References

Chandwani2004 R. Chandwani, K. A. Jordan, B. L. Shacklett, E. Papasavvas, L. J. Montaner, M. G. Rosenberg, D. F. Nixon, and J. K. Sandberg. Limited Magnitude and Breadth in the HLA-A2-Restricted CD8 T-Cell Response to Nef in Children with Vertically Acquired HIV-1 Infection. Scand. J. Immunol., 59(1):109-114, Jan 2004. PubMed ID: 14723629. Show all entries for this paper.

Displaying record number 58953

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HXB2 Location	Nef(42-50) DNA(8920..8946)	Nef Epitope Map
Author Location	Nef(42-50)
Epitope	ALTSSNTAA	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	CAP063: A*02:01, A*23:01, B*45:01, C*04:01, C*16:01
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	escape, acute/early infection

Notes

• Amino acid mutations in HIV-1 subtype C were tracked within the first 6 months following transmission in 5 subjects. Early, rapid adaptations (55 mutations) to the viral genome were predominantly driven by CTL pressure (38%), but also by antibody pressure (35%) and reversions (16%). Most CTL escape occurred in Nef, followed by Pol and Env.
• Epitope ALTSSNTAA underwent mutations at amino acids S, positions 3 and 4, A positions 8 and 9. These changes were first seen 5-11 weeks after transmission. The escapes were tracked in subject CAP63.
• Using autologous peptides, 13/21 putative epitopes were confirmed to be responsive [see Liu et al. J. Clinical Investigation. 123:380-93 (2013) for details]

References

Abrahams2013 Melissa-Rose Abrahams, Florette K. Treurnicht, Nobubelo K. Ngandu, Sarah A. Goodier, Jinny C. Marais, Helba Bredell, Ruwayhida Thebus, Debra de Assis Rosa, Koleka Mlisana, Cathal Seoighe, Salim Abdool Karim, Clive M. Gray, and Carolyn Williamson. Rapid, Complex Adaptation of Transmitted HIV-1 Full-Length Genomes in Subtype C-Infected Individuals with Differing Disease Progression. AIDS, 27(4):507-518, 20 Feb 2013. PubMed ID: 23370465. Show all entries for this paper.