Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 58227

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HXB2 Location	Vif(119-127) DNA(5395..5421)	Vif Epitope Map
Author Location	Vif(119-127)
Epitope	AIRKAILGR	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(A*31:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	CH040: A*02:01, A*31:01, B*40:01, B*44:02, C*03:02, C*05:01
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	dynamics, escape, acute/early infection, autologous responses, characterizing CD8+ T cells

Notes

• Multifunctionality of CTL against transmitted or founder HIV-1 isolates during early or acute infection was examined for correlation with escape mutation selection. The earliest CTL responses were oligofunctional, and progressively became more multifunctional. A significant association was found between total CTL response and order of escape selection, and between epitope entropy with the rate of emergence of escape mutants.
• 18-mer DCFSESAIRKAILGRIVS (Vif 113-129) contains epitope AIRKAILGR, that shows early evidence of escape by 181 days from onset of symptoms (DFOSx). This epitope elicited CTL response in patient CH40.

References

Ferrari2011 Guido Ferrari, Bette Korber, Nilu Goonetilleke, Michael K. P. Liu, Emma L. Turnbull, Jesus F. Salazar-Gonzalez, Natalie Hawkins, Steve Self, Sydeaka Watson, Michael R. Betts, Cynthia Gay, Kara McGhee, Pierre Pellegrino, Ian Williams, Georgia D. Tomaras, Barton F. Haynes, Clive M. Gray, Persephone Borrow, Mario Roederer, Andrew J. McMichael, and Kent J. Weinhold. Relationship between Functional Profile of HIV-1 Specific CD8 T Cells and Epitope Variability with the Selection of Escape Mutants in Acute HIV-1 Infection. PLoS Pathog., 7(2):e1001273, 2011. PubMed ID: 21347345. Show all entries for this paper.

Displaying record number 56658

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HXB2 Location	Vif(119-127) DNA(5395..5421)	Vif Epitope Map
Author Location	Vif(119-127)
Epitope	AIRKAILGR	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	A*02:01, A*31:01, B*40:01, B*44:02, C*03:02, C*05:01
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	escape, acute/early infection

Notes

• Using overlapping 15-mer peptides, CTL responses to early, acute HIV infection were determined. Early virus escape attributed to CTL response was charted and subsequent clusters of mutations due to NAb targeting were also found.
• Five findings made: (i) exact numbers of transmitted HIV-1 actual and potential T cell epitopes were determined (ii) CTL-mediated virus escape occurring as viremia peaks, drops and reaches set point earlier and faster than previously known (iii) CTL responses to some transmitted virus epitopes were lost after early escapes were selected (iv) a few unexplained escapes were probably due to bystander mutations (v) earliest CTL responses cause the decline of VL in acute infection.
• This transmitted epitope, AIRKAILGR, studied as part of peptide dcfsesAIRKAILGRiv, was recognized in patient CH40. Unrecognized mutants, AIRKAlLGR, AIRKAIvGR, AIRtAILGR, AIReAILGR and dcfsDsAIReAILGRiv were seen from day 111 on. There is statistical evidence for variation and escape in this region.

References

Goonetilleke2009 Nilu Goonetilleke, Michael K. P. Liu, Jesus F. Salazar-Gonzalez, Guido Ferrari, Elena Giorgi, Vitaly V. Ganusov, Brandon F. Keele, Gerald H. Learn, Emma L. Turnbull, Maria G Salazar, Kent J Weinhold, Stephen Moore, CHAVI Clinical Core B, Norman Letvin, Barton F. Haynes, Myron S. Cohen, Peter Hraber, Tanmoy Bhattacharya, Persephone Borrow, Alan S. Perelson, Beatrice H. Hahn, George M. Shaw, Bette T. Korber, and Andrew J. McMichael. The first T cell response to transmitted/founder virus contributes to the control of acute viremia in HIV-1 infection. J. Exp. Med., 206(6):1253-1272, 8 Jun 2009. PubMed ID: 19487423. Show all entries for this paper.

Displaying record number 61055

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HXB2 Location	Vif(119-127) DNA(5395..5421)	Vif Epitope Map
Author Location	Vif(119-127)
Epitope	AIRKAILGR	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	CH040: A*02:01, A*31:01, B*40:01, B*44:02, C*03:02, C*05:01
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	computational epitope prediction, escape, acute/early infection, mutation acquisition

Notes

• 17 patients with acute HIV-1 infection were followed to validate that (1) relative escape rate (2) location and identity of escape mutations and (3) epistatic effects on escape, could be predicted within a host by using a simple model based on population genetics and known fitness measures of HIV polyproteins. In general escape location was found most at residues with the lowest fitness cost and escape time correlates with early detection of mutants when T cell response was first detected.
• Epitope AIRKAILGR had a fitness cost ΔE of -0.572 and T cell response was first detected to it at 16d, escape time was 171.97 days.

References

Barton2016 John P. Barton, Nilu Goonetilleke, Thomas C. Butler, Bruce D. Walker, Andrew J. McMichael, and Arup K. Chakraborty. Relative Rate and Location of Intra-Host HIV Evolution to Evade Cellular Immunity Are Predictable. Nat. Commun., 7:11660, 23 May 2016. PubMed ID: 27212475. Show all entries for this paper.