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Displaying record number 52470

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HXB2 Location	Gag(163-176) p24(31-44) DNA(1276..1317)	Gag Epitope Map
Author Location	p24(31-44 HXB2)
Epitope	AFSPEVIPMFSALS	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Experimental methods	T-cell Elispot
Keywords	supervised treatment interruptions (STI), immunodominance, early treatment

Notes

• Comprehensive Elispot screening of 504 overlapping peptides that spanned all HIV-1 proteins in 57 HIV-1 infected individuals. Four groups of patients were compared: 23 were untreated; 12 patients were chronically infected and treated; 22 started treatment during acute infection, 11 continuously treated and 11 with STI.
• 63% of the peptides were recognized - the most frequent responses were directed against Nef (95%) and p24-Gag (88%). p17 was the most frequently recognized protein after correction for protein length. A median of 18 peptides (range 2-42) were recognized per person. The most frequently (>20%) recognized peptides were located in conserved regions within clade B sequences. Vpu was rarely recognized.
• A decrease in the total magnitude and in the breadth of CTL responses was detected in patients with treated acute infection in comparison to chronically infected treated or untreated individuals. No correlation between plasma viral load and HIV-1 specific T-cell responses was observed.
• The authors did not note the reference strain, but based on the peptide sequences provided, it appears to be HXB2.
• Responses to this peptide were detected in 22% of the study subjects, and it was one of the 25 most frequently recognized peptides.

References

Addo2003 M. M. Addo, X. G. Yu, A. Rathod, D. Cohen, R. L. Eldridge, D. Strick, M. N. Johnston, C. Corcoran, A. G. Wurcel, C. A. Fitzpatrick, M. E. Feeney, W. R. Rodriguez, N. Basgoz, R. Draenert, David R. Stone, C. Brander, P. J. R. Goulder, E. S. Rosenberg, M. Altfeld, and B. D. Walker. Comprehensive epitope analysis of human immunodeficiency virus type 1 (HIV-1)-specific T-cell responses directed against the entire expressed HIV-1 genome demonstrate broadly directed responses, but no correlation to viral load. J. Virol., 77(3):2081-2092, Feb 2003. PubMed ID: 12525643. Show all entries for this paper.