Search CTL/CD8+ T-Cell Epitope Database

Found 8 matching records:

Displaying record number 53421

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HXB2 Location	Gag(210-218) p24(78-86) DNA(1417..1443)	Gag Epitope Map
Author Location	p24(78-86)
Epitope	`AEWDRLHPV`	Epitope Alignment
		Show epitope variants
Epitope Name	AEW
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Patient MHC/HLA	A2, A68.1, B07, B35:03, C04:01, C07:02, DQ2, DQ6, DR15, DR17, DR51, DR52
Experimental methods	CD4 T-cell Elispot - IFNγ, CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, escape

Notes

The increase in plasma viral load in a patient that progressed rapidly was preceded by positive selection of viral escape mutations in epitopes targeted by dominant HIV-1-specific CD8+ T-cell responses,and a decrease in HIV-1-specific CD4+ and CD8+ T-cell frequencies. Overall, escape variant epitopes were recognized 0-80% as efficiently as the index peptide, and the relative efficiency of the variant epitopes increased using PBLs collected after their appearance. No changes were found in viral tropism, replication kinetics and neutralizing antibody titers, so the rapid decline of the patient was attributed to loss of HIV containment due to CTL escape.
This was one of 8 reactive epitopes found not to vary over time.

References

Oxenius2004 Annette Oxenius, David A. Price, Alexandra Trkola, Charles Edwards, Emma Gostick, Hua-Tang Zhang, Philippa J. Easterbrook, Tin Tun, Andrew Johnson, Anele Waters, Edward C. Holmes, and Rodney E. Phillips. Loss of Viral Control in Early HIV-1 Infection Is Temporally Associated with Sequential Escape from CD8+ T Cell Responses and Decrease in HIV-1-Specific CD4+ and CD8+ T Cell Frequencies. J. Infect. Dis., 190(4):713-721, 15 Aug 2004. PubMed ID: 15272399. Show all entries for this paper.

Displaying record number 57071

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HXB2 Location	Gag(210-218) p24(78-86) DNA(1417..1443)	Gag Epitope Map
Author Location	p24(78-86 DU422)
Epitope	`AEWDRLHPV`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	in vitro stimulation or selection
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Tetramer binding
Keywords	dendritic cells

Notes

Three types of myeloid dendritic cells - monocytic dendritic cells, Langerhans cells, and interstitial dermal-intestinal dendritic cells - were shown to be able to prime CD8+ T-cell responses to HIV-1. Primed monocytic dendritic cells from 2 uninfected adult donors had broad primary T-cell reactivity, reacting with 15mers containing 42 known epitopes in Gag, Env, and Nef.
One of the reactive 15mers contained optimal epitope AEWDRLHPV, which was restricted by HLA A2.

References

Colleton2009 Bonnie A. Colleton, Xiao-Li Huang, Nada M. Melhem, Zheng Fan, Luann Borowski, Giovanna Rappocciolo, and Charles R. Rinaldo. Primary Human Immunodeficiency Virus Type 1-Specific CD8+ T-Cell Responses Induced by Myeloid Dendritic Cells. J. Virol., 83(12):6288-6299, Jun 2009. PubMed ID: 19357176. Show all entries for this paper.

Displaying record number 54856

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HXB2 Location	Gag(210-218) p24(78-86) DNA(1417..1443)	Gag Epitope Map
Author Location	p24( Henan isolate)
Epitope	`AEWDRLHPV`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords

Notes

CTL responses were studied in treated and untreated donors from China, using Gag peptides from Henan isolate. All peptides were predicted to have A2 or A11 restriction (two of the most prominent alleles in Chinese population) and HLA restriction was further analyzed by CTL recognition in patients carrying corresponding allele. Among treated donors, HLA-A2 donors had higher CTL response than than HLA-A11 donors. Chronically infected untreated individuals had higher magnitude of CTL response than treated ones.
AEWDRLHPV was among 5 mostly recognized epitopes (69%).

References

Gong2006 Xiaoyan Gong, Xien Gui, Yuxia Zhang, and Po Tien. Screening for CD8 Cytotoxic T Lymphocytes Specific for Gag of Human Immunodeficiency Virus Type 1 Subtype B' Henan Isolate from China and Identification of Novel Epitopes Restricted by the HLA-A2 and HLA-A11 Alleles. J. Gen. Virol., 87(Pt 1):151-158, Jan 2006. PubMed ID: 16361427. Show all entries for this paper.

Displaying record number 55266

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HXB2 Location	Gag(210-218) p24(78-86) DNA(1417..1443)	Gag Epitope Map
Author Location	p24(78-86)
Epitope	`AEWDRLHPV`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*40)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	assay or method development, optimal epitope

Notes

HLA-A11-restricted CTL epitopes in 2 male Han Chinese subjects (one ART naive and one receiving HAART) were determined. A novel epitope was discovered and 5 other putative epitopes confirmed using 425 overlapping peptides spanning the entire HIV-1 clade B proteome. By employing intracellular cytokine staining, the authors developed a new method of mapping optimal epitopes and defining their HLA restriction efficiently. In spite of its being the most common HLA type in China, A*11-restricted epitopes were recognized less frequently than other epitopes, and A*11-restricted responses formed a mean of only 13% of the responses against HIV.
This epitope, AEWDRLHPV, was detected within overlapping peptides LKETINEEAAEWDRLHPV and AAEWDRLHPVHAGPIA.

References

Wang2007 Shaoyang Wang, Yongtao Sun, Song Zhai, Yan Zhuang, Shuguang Zhao, Whenzhen Kang, Xinhong Li, Dedong Huang, Xu G. Yu, Bruce D. Walker, and Marcus A. Altfeld. Identification of HLA-A11-Restricted HIV-1-Specific Cytotoxic T-Lymphocyte Epitopes in China. Curr. HIV Res., 5(1):119-128, Jan 2007. PubMed ID: 17266563. Show all entries for this paper.

Displaying record number 55718

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HXB2 Location	Gag(210-218) p24(78-86) DNA(1417..1443)	Gag Epitope Map
Author Location	Gag
Epitope	`AEWDRLHPV`	Epitope Alignment
Epitope Name	AV9-B40
Subtype	B, F
Species (MHC/HLA)	human(B40)
Immunogen	HIV-1 infection
Country	Argentina
Experimental methods
Keywords	dynamics, HLA associated polymorphism

Notes

Dynamics of CTL escape mutations was studied theoretically by analyzing plasma samples from 302 subjects (collected over 20 years). HLA associated polymorphisms accumulated in a subtype-specific manner.
Epitope AEWDRLHPV with anchor residues at A(E)WDRLHP(V) mutates to variant AEWDRLHPa. The consensus epitope AEWDRLHPl increases with time.

References

Dilernia2008 Dario Alberto Dilernia, Leandro Jones, Sabrina Rodriguez, Gabriela Turk, Andrea E Rubio, Sandra Pampuro, Manuel Gomez-Carrillo, Christian Bautista, Gabriel Deluchi, Jorge Benetucci, María Beatriz Lasala, Leonardo Lourtau, Marcelo Horacio Losso, Héctor Perez, Pedro Cahn, and Horacio Salomón. HLA-Driven Convergence of HIV-1 Viral Subtypes B and F Toward the Adaptation to Immune Responses in Human Populations. PLoS ONE, 3(10):e3429, 2008. PubMed ID: 18941505. Show all entries for this paper.

Displaying record number 58493

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HXB2 Location	Gag(210-218) p24(78-86) DNA(1417..1443)	Gag Epitope Map
Author Location	Gag(210-218)
Epitope	`AEWDRLHPV`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*40:02)
Immunogen	HIV-1 infection
Country	Canada
Experimental methods	Other
Keywords	characterizing CD8+ T cells, elite controllers

Notes

Dual color EliSpot testing for IL-2 and IFN-γ was used to determine whether CTL restricted by MHC Class I alleles associated with slow progression to disease, viz. B*57 and B*27, had a more poly-functional profile than other CD8+ cells in a treatment-naive cohort from Montreal. B*57/B*27-restricted CTL producing IL-2 or IFN-γ or IL-2/IFN-γ, were similar in both slow progressors (SP) and progressors. Other non-protective MHC-I-restricted CTL producing both IL-2/IFN-γ, were greater in number in SP than progressors.
Within SP, peptides restricted by both protective and non-protective MHC-I alleles elicited CTL with the same functional profile. For progressors, peptides and epitopes restricted by protective B*57/B*27 alleles stimulated a higher proportion of dual-(IL-2/IFN-γ)secreting CTL.
HLA-B*4002-restricted Gag optimal epitope AEWDRLHPV was tested in a dual color EliSpot and found to elicit CTL that could produce IL-2 and/or IFN-γ.

References

Peretz2011 Yoav Peretz, Olivia Marra, Réjean Thomas, Danielle Legault, Pierre Côté, Mohamed-Rachid Boulassel, Danielle Rouleau, Jean-Pierre Routy, Rafick-Pierre Sékaly, Christos M Tsoukas, Cécile Tremblay, and Nicole F. Bernard. Relative Contribution of HIV-Specific Functional Lymphocyte Subsets Restricted by Protective and Non-Protective HLA Alleles. Viral Immunol, 24(3):189-198, Jun 2011. PubMed ID: 21668360. Show all entries for this paper.

Displaying record number 61534

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HXB2 Location	Gag(210-218) p24(78-86) DNA(1417..1443)	Gag Epitope Map
Author Location	p24(78-86)
Epitope	`AEWDRLHPV`	Epitope Alignment
		Show epitope variants
Epitope Name	Gag AV9
Subtype	B
Species (MHC/HLA)	human(B*40:02)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	subtype comparisons, variant cross-reactivity, optimal epitope, novel epitope, chronic infection

Notes

Polymorphic epitope regions can be targets for cross-clade CTL response. In addition to 6 conserved AE epitopes, 9 epitopes polymorphic between clades AE and B were identified in chronically infected Japanese subjects. This is the first comprehensive study to identify cross-clade CTL by using overlapping HIV-1 peptides.
There were no significant differences in total magnitude of CTL response between 26 clade A/E-infected and 401 clade B-infected subjects when tested with 11-mers from HIV-B Nef, Gag and Pol. 6 epitopes were novel viz. Gag LL11, Pol SV9, Pol GI9, Gag RV8, Gag AP8 and Gag EG11.
Clade B epitope Gag p24 AV9, AEWDRLHPV, was found in 1135/1644 Clade B and 47/315 Clade A/E Los Alamos database HIV-1 sequences. It was found in 1/26 A/E-infected subjects of the KI cohort. CTL induced by this clade B peptide were able to recognize clades B and A/E peptides evenly.
CTL from A/E-infected subjects stimulated by the consensus B AV9 peptide used cross-recognized both clades B and A/E infected cells.

References

Watanabe2013 Koji Watanabe, Hayato Murakoshi, Yoshiko Tamura, Madoka Koyanagi, Takayuki Chikata, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi. Identification of Cross-Clade CTL Epitopes in HIV-1 Clade A/E-Infected Individuals by Using the Clade B Overlapping Peptides. Microbes Infect., 15(13):874-886, Nov 2013. PubMed ID: 23968885. Show all entries for this paper.

Displaying record number 53894

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HXB2 Location	Gag(210-218) p24(78-86) DNA(1417..1443)	Gag Epitope Map
Author Location	p24(78-86)
Epitope	`AEWDRLHPV`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*40:06)
Immunogen	HIV-1 infection
Country	India
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, computational epitope prediction, immunodominance

Notes

PBMCs from HIV-1 infected Indian subjects were tested for HIV-1 Gag (with C clade peptides), Nef (with B clade peptides), and Env (clade B peptides) specific T-cell responses. 5 immunodominant regions conserved across clades were identified in Gag and Nef. 3 antigenic regions were found to be recognized by CTLs in the Indian subjects; these regions were not identified as immunodominant in studies from Africa.
26 epitopes were predicted within reactive peptides, of which 90% were clustered in the conserved immunodominant regions of Gag and Nef.
3 of the epitopes were highly conserved across clades, and 7 novel epitopes were found.
Epitope AEWDRLHPV showed >50% conservation across clades with >90% conservation to subtype D sequence. This epitope was restricted to HLA-B*4006 in one subject and to HLA-B*4006 or -Cw*0602 in another subject.

References

Thakar2005 Madhuri R. Thakar, Leena S. Bhonge, Samir K. Lakhashe, U. Shankarkumar, Suvarna S. Sane, Smita S. Kulkarni, Bharati A. Mahajan, and Ramesh S. Paranjape. Cytolytic T Lymphocytes (CTLs) from HIV-1 Subtype C-Infected Indian Patients Recognize CTL Epitopes From a Conserved Immunodominant Region of HIV-1 Gag and Nef. J. Infect. Dis., 192(5):749-759, 1 Sep 2005. PubMed ID: 16088824. Show all entries for this paper.