Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 59007

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HXB2 Location	Pol(592-600) RT(437-445) DNA(3858..3884)	Pol Epitope Map
Author Location	Pol(592-)
Epitope	AETFYVDGA	Epitope Alignment
Epitope Name	Pol592
Subtype	A
Species (MHC/HLA)	human(B*44)
Immunogen	HIV-1 infection, vaccine
Patient MHC/HLA	P4: A*30:02, A*68:01, B*56:01, B*58:01; P7: A*26:01, A*68:01, B*08:01
Country	Guinea-Bissau
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses, vaccine antigen design, therapeutic vaccine, chronic infection

Vaccine Details

Notes

• Therapeutic vaccination of chronically infected HIV-1 subjects was evaluated as a means of halting disease progression. A subdominant peptide vaccine containing 15 CTL epitopes and 3 helper T-cell peptides along with an adjuvant was delivered to 18 infected subjects, and this Guinea-Bissau study compared to a similar Danish one. Using IFN-γ EliSpot, 9 new T-cell responses were detected in 6/14 subjects whose viral loads (VL) remained stable. Other pre-existing responses, including CTL responses, however, were not significantly detected.
• Pol592, AETFYVDGA, is a CTL epitope sequence used as a vaccine component.

References

Roman2013 Victor Raúl Gómez Román, Kristoffer Jarlov Jensen, Sanne Skov Jensen, Christian Leo-Hansen, Sanne Jespersen, David da Silva Té, Candida Medina Rodrigues, Christoph Mikkel Janitzek, Lasse Vinner, Terese Lea Katzenstein, Peter Andersen, Ingrid Kromann, Lars Vibe Andreasen, Ingrid Karlsson, and Anders Fomsgaard. Therapeutic Vaccination Using Cationic Liposome-Adjuvanted HIV Type 1 Peptides Representing HLA-Supertype-Restricted Subdominant T Cell Epitopes: Safety, Immunogenicity, and Feasibility in Guinea-Bissau. AIDS Res Hum Retroviruses, 29(11):1504-1512, Nov 2013. PubMed ID: 23634822. Show all entries for this paper.

Displaying record number 58420

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HXB2 Location	Pol(592-600) RT(437-445) DNA(3858..3884)	Pol Epitope Map
Author Location	Pol
Epitope	AETFYVDGA	Epitope Alignment
Epitope Name	Pol592
Subtype	B, CRF02_AG
Species (MHC/HLA)	human(B44)
Immunogen	HIV-1 infection
Country	Cote D'Ivoire, Denmark, Ghana, Guinea-Bissau, Mali, Senegal
Experimental methods	Intracellular cytokine staining, Pentamer labeling
Keywords	vaccine antigen design

Notes

• 15 subdominant CTL epitopes were used to study 2 new Guinea-Bissau HIV-1 isolates, a newly completed Danish subtype B genome sequence and other previously published West African sequences. Subtype analysis showed the 2 new Guinea-Bissau sequences were CRF02_AG inter-subtype recombinant forms with typical cross-over points. The 15 epitopes were restricted by common HLA alleles (A1, A2, A3, B7, B44) and were present and conserved in all sequences studied. Mean conservation of the epitopes in local strains was 76% and 70% for Denmark and West Africa respectively. All epitopes had been previously confirmed as antigenic [Perez2008].
• Pol592 epitope, AETFYVDGA, showed a conservation of amino acid identity of 94% for the Danish sequence and 86% for the West African sequences.

References

Perez2008 Carina L. Pérez, Mette V. Larsen, Rasmus Gustafsson, Melissa M. Norström, Ann Atlas, Douglas F. Nixon, Morten Nielsen, Ole Lund, and Annika C. Karlsson. Broadly Immunogenic HLA Class I Supertype-Restricted Elite CTL Epitopes Recognized in a Diverse Population Infected with Different HIV-1 Subtypes. J. Immunol., 180(7):5092-5100, 1 Apr 2008. PubMed ID: 18354235. Show all entries for this paper.

Vinner2011 Lasse Vinner, Birgitta Holmgren, Kristoffer J. Jensen, Joakim Esbjornsson, Marie Borggren, Julie L. Hentze, Ingrid Karlsson, Betina S. Andresen, Gregers J. Gram, Henrik Kloverpris, Peter Aaby, Zacarias José Da Silva, Eva-Maria Fenyö, and Anders Fomsgaard. Sequence Analysis of HIV-1 Isolates from Guinea-Bissau: Selection of Vaccine Epitopes Relevant in Both West African and European Countries. APMIS, 119(8):487-497, Aug 2011. PubMed ID: 21749448. Show all entries for this paper.

Displaying record number 1972

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HXB2 Location	Pol(592-600) RT(437-445) DNA(3858..3884)	Pol Epitope Map
Author Location	RT(437-445)
Epitope	AETFYVDGA	Epitope Alignment
Epitope Name	Pol-AA9; RT-AA9
Subtype	B
Species (MHC/HLA)	human(B*45:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*30:02, A*32:01, B*45:01, B*53:01, C*04:01, C*12:02; 00RCH33: A*30:02, A*32:01, B*45:01, B*53:01, C*04:01, C*12:02
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	HAART, ART

Notes

• This study monitored epitope responses in HIV-1 infected minority women living in the United States.
• 24 epitopes were described -- 8 were novel, 8 used new restricting elements but were previously defined epitopes, and 8 were previously described.
• Serial peptide truncations were used to define optimal epitopes for CTL cell lines isolated from 12 individuals, assayed by a Cr-release.
• Subject 00RCH33 was on HAART had a viral load of 2900 and CD4 count of 727 and also recognized the epitopes YPLTFGWCY, Nef(135-143), HLA B*5301; RSLYNTVATLY, p17(76-86), HLA A*3002; and HIGPGRAFY, gp160(310-318), HLA A*3002.
• Among HIV+ individuals who carried HLA B45, 3/9 (33%) recognized this previously described epitope.

References

Sabbaj2003 Steffanie Sabbaj, Anju Bansal, G. Douglas Ritter, Christopher Perkins, Bradley H. Edwards, Ethan Gough, Jianming Tang, James J. Szinger, Bette Korber, Craig M. Wilson, Richard A. Kaslow, Mark J. Mulligan, and Paul A. Goepfert. Cross-Reactive CD8+ T Cell Epitopes Identified in US Adolescent Minorities. J. Acquir. Immune Defic. Syndr., 33(4):426-438, 1 Aug 2003. PubMed ID: 12869831. Show all entries for this paper.

Displaying record number 61959

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HXB2 Location	Pol(592-600) RT(437-445) DNA(3858..3884)	Pol Epitope Map
Author Location	Pol(592-)
Epitope	AETFYVDGA	Epitope Alignment
Epitope Name	AA9
Subtype	A3, B, C, CRF02_AG
Species (MHC/HLA)	human(B*45:01, B*50:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	1108: ; 1115: A*02:01, A*68:01, B*50:01, B*58:01
Country	Denmark, Guinea-Bissau
Experimental methods	HLA binding, Intracellular cytokine staining, Tetramer binding
Keywords	binding affinity, vaccine antigen design

Notes

• 95% and 100% of untreated, asymptomatic HIV-1-infected patients, from Guinea-Bissau and Denmark respectively, were able to subdominantly recognize 15 predicted CTL epitopes restricted by at least one of the 5 most common HLA supertypes, HLA-A*01, -A*02, -A*03, -B*07 and -B*44.
• Epitope AA9, AETFYVDGA, predicted and validated to be restricted by supertype B44, was found across geographic regions in 94% of HIV-1 sequences in Denmark clade-B and 86% Guinea-Bissau/Western Africa subtypes A3, C and CRF02_AG. Its equivalent peptide, Pol311 was able to elicit cytokine production and bound the following HLA alleles moderately or well: B*4501, B*5001 (B44 supertype).

References

Karlsson2012 Ingrid Karlsson, Henrik Kløverpris, Kristoffer Jarlov Jensen, Anette Stryhn, Søren Buus, Annika Karlsson, Lasse Vinner, Philip Goulder, and Anders Fomsgaard. Identification of Conserved Subdominant HIV Type 1 CD8+ T Cell Epitopes Restricted within Common HLA Supertypes for Therapeutic HIV Type 1 Vaccines. AIDS Res. Hum. Retroviruses, 28(11):1434-1443, Nov 2012. PubMed ID: 22747336. Show all entries for this paper.

Displaying record number 56953

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HXB2 Location	Pol(592-600) RT(437-445) DNA(3858..3884)	Pol Epitope Map
Author Location	Pol
Epitope	AETFYVDGA	Epitope Alignment
Subtype	A1
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Sweden
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, computational epitope prediction, vaccine antigen design

Notes

• From HIV-1 sequences of multiple subtypes, bioinformatic prediction generated 174 CTL epitopes from Gag, Pol, Nef, Env and Tat restricted by HLA supertypes A1, A2, A3, A24, B7, B44 and B58; 10 previously-published A2- and B58-restricted epitopes were also included. Of these 184 epitopes, 113 were recognized experimentally in serum from 31 HIV+ patients.
• While Gag, Pol, Env and Nef were highly immunogenic, only 38% of Tat epitopes elicited a response.
• AETFYVDGA was a predicted epitope restricted by B44 supertype. 1/31 patient(s) responded to it; the patient was known to carry an allele of B44 supertype. The responding patient was infected with HIV clade A1.

References

Perez2008 Carina L. Pérez, Mette V. Larsen, Rasmus Gustafsson, Melissa M. Norström, Ann Atlas, Douglas F. Nixon, Morten Nielsen, Ole Lund, and Annika C. Karlsson. Broadly Immunogenic HLA Class I Supertype-Restricted Elite CTL Epitopes Recognized in a Diverse Population Infected with Different HIV-1 Subtypes. J. Immunol., 180(7):5092-5100, 1 Apr 2008. PubMed ID: 18354235. Show all entries for this paper.