Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 52651

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HXB2 Location	Nef(83-92) DNA(9043..9072)	Nef Epitope Map
Author Location	Nef(83-92)
Epitope	AAVDLSHFLK	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*02:01, A11, B51, B61, C*14, Cw2
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	acute/early infection, early treatment

Notes

• CTL epitope responses were mapped in 21 men within 15-92 days post-HIV-1 infection. Subjects initially showed a narrow immune response with a mean of 2.3 epitopes recognized per patient. The immune response broadened later in infection. No correlation between the plasma viral load and the number of recognized epitopes or the frequency of IFNγ-secreting cells was observed, and there was no correlation between the functional avidity of a response and the abundance of IFNγ-secreting cells that recognized the epitope. The first epitopes to be recognized tended not to be the most avid, and earliest responses tended to be directed against Nef, Tat, Vpr, and Env.
• More common HLA alleles were less frequently used in primary infection than less common alleles, for example A3, B35, B57, and B62 were more frequently utilized than A1, A2, A30, and A44.
• All HIV-1 proteins except Vpu were recognized, and responses to a total of 41 optimal epitopes were characterized; 24 had been identified previously. 48% of the total T-cell responses were directed against Nef and 43% were directed against Gag.

References

Cao2003 Jianhong Cao, John McNevin, Sarah Holte, Lisa Fink, Lawrence Corey, and M. Juliana McElrath. Comprehensive Analysis of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific Gamma Interferon-Secreting CD8+ T Cells in Primary HIV-1 Infection. J. Virol., 77(12):6867-6878, Jun 2003. PubMed ID: 12768006. Show all entries for this paper.

Displaying record number 54546

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HXB2 Location	Nef(83-92) DNA(9043..9072)	Nef Epitope Map
Author Location	Nef(83-92)
Epitope	AAVDLSHFLK	Epitope Alignment
		Show epitope variants
Epitope Name	AK10
Subtype	B
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	escape, acute/early infection, assay or method development, mutation acquisition

Notes

• The ability of T cells from early infected subjects to recognize Nef clade B HIV-1 epitope variants was tested using both a consensus peptide panel and a standardized potential T-cell epitope panel (PTE-B). The magnitude and breadth of PTE-optimized peptide panels was significantly higher than CON peptides'. 29 potential epitopic domains were detected in Nef using the PTE peptide set.
• 2 PTE-B peptide sequences were identified, PMTYKAAVDLSHFLK and GAlDLSHFLKEKGGL, containing variants of this consensus epitope sequence AK10, viz. AAVDLSHFLK and GAlDLSHFLK, both of which elicited IFN-gamma immune responses.
• HLA-A11 restriction for AK10 was presumed based on the subject's having the HLA allele and publication in the Los Alamos database.

References

Malhotra2007a Uma Malhotra, Fusheng Li, Jessica Nolin, Megan Allison, Hong Zhao, James I. Mullins, Steve Self, and M. Juliana McElrath. Enhanced Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Nef-Specific T Cells Recognizing Multiple Variants in Early HIV-1 Infection. J. Virol., 81(10):5225-5237, May 2007. PubMed ID: 17329342. Show all entries for this paper.

Displaying record number 59490

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HXB2 Location	Nef(83-92) DNA(9043..9072)	Nef Epitope Map
Author Location	Nef( pNL4-3)
Epitope	AAVDLSHFLK	Epitope Alignment
Epitope Name	AK10
Subtype	B
Species (MHC/HLA)	human(A*11:01)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, TCR binding
Keywords	assay or method development, novel epitope

Notes

• Deep Ligand Sequencing (DLS) was used to identify 37 novel HLA-A*1101-restricted epitopes, and 5 previously published ones. HLA-A*1101-expressing CD4⁺ T cells were infected with HIV strain pNL4-3; soluble HLA-A*1101-ligand was recovered and purified, and then ligands (i.e. epitopes) were acid-released. A combination of chromatography and mass spectrometry used to separate the distinct peptides. For each epitope, the HLA binding affinity and median EliSpot response in 23 A*1101-positive patients were determined. This is the first report of direct elution of HIV-1 genome-encoded epitopes.
• Gag and N-terminal Nef epitopes dominate the antigenic profile of epitopes over other HIV-1 proteins (Pol, Env, Vpu). Nef and Pol epitopes, however, were widely recognized. Immunologically reactive epitopes are often atypical in length, particularly in epitope hotspots of Gag and Nef. Epitopes discovered were primarily a result of de novo protein synthesis in the host cell instead of secretory pathway peptide processing.
• AAVDLSHFLK, AK10, had high TCR binding and was recognized by 12/23 A*1101-positive patients with a median magnitude of 461 SFU/10⁶ PBMC. It is located in the primary Nef hotspot of reactive epitopes.

References

Yaciuk2014 Jane C. Yaciuk, Matthew Skaley, Wilfried Bardet, Fredda Schafer, Danijela Mojsilovic, Steven Cate, Christopher J. Stewart, Curtis McMurtrey, Kenneth W. Jackson, Rico Buchli, Alex Olvera, Samandhy Cedeño, Montserrat Plana, Beatriz Mothe, Christian Brander, John T. West, and William H. Hildebrand. Direct Interrogation of Viral Peptides Presented by the Class I HLA of HIV-Infected T Cells. J. Virol., 88(22):12992-13004, Nov 2014. PubMed ID: 25165114. Show all entries for this paper.