Search CTL/CD8+ T-Cell Epitope Database

Found 7 matching records:

Displaying record number 53148

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HXB2 Location	Gag(77-85) p17(77-85) DNA(1018..1044)	Gag Epitope Map
Author Location	p17(77-85)
Epitope	`SLYNTVATL`	Epitope Alignment
Epitope Name	SL9
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods	Chromium-release assay
Keywords	binding affinity, TCR usage, characterizing CD8+ T cells

Notes

Killing efficiency of CTLs and their ability to suppress viral replication is shown to depend more on epitope specificity than to antigenic avidity. Different clones recognizing the same epitope had similar killing efficiency despite their variation in avidity. Nef specific CTL clones tended to be most inhibitory, followed by Gag, then by RT specific clones, regardless of avidity.
3/14 CTL T-cell clones tested were specific for Gag/p17-SL9. Under conditions of excess peptide (100ug/ml), there was no difference in their lytic potential; all possessed similar effector capacity. Avidity was measured as the sensitizing dose of peptide required for 50% of maximal killing (SD50), which varied from 20 pg/ml to 100 ng/ml, over four orders of magnitude for all 14 epitopes. The SD50 range for Gag/p17-SL9 was 1,000 - 20,000 pg/ml.

References

Yang2003a Otto O. Yang, Phuong T. Nguyen Sarkis, Alicja Trocha, Spyros A. Kalams, R. Paul Johnson, and Bruce D. Walker. Impacts of Avidity and Specificity on the Antiviral Efficiency of HIV-1-Specific CTL. J. Immunol., 171(7):3718-3724, 1 Oct 2003. PubMed ID: 14500671. Show all entries for this paper.

Displaying record number 53149

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HXB2 Location	Gag(92-101) p17(92-101) DNA(1063..1092)	Gag Epitope Map
Author Location	p17(92-101)
Epitope	`IEIKDTKEAL`	Epitope Alignment
Epitope Name	Gag/p17-IL10
Subtype	B
Species (MHC/HLA)	human(B60)
Immunogen	HIV-1 infection
Experimental methods	Chromium-release assay
Keywords	binding affinity, epitope processing, TCR usage, characterizing CD8+ T cells

Notes

Killing efficiency of CTLs and their ability to suppress viral replication is shown to depend more on epitope specificity than to antigenic avidity. Different clones recognizing the same epitope had similar killing efficiency despite their variation in avidity. Nef specific CTL clones tended to be most inhibitory, followed by Gag, then by RT specific clones, regardless of avidity.
1/14 CTL T-cell clones tested were specific for Gag/p17-IL10. Under conditions of excess peptide (100ug/ml), there was no difference in their lytic potential; all possessed similar effector capacity. Avidity was measured as the sensitizing dose of peptide required for 50% of maximal killing (SD50), which varied from 20 pg/ml to 100 ng/ml, over four orders of magnitude for all 14 epitopes. The SD50 value for the Gag/p17-IL10 clone was 8,000 pg/ml.

References

Displaying record number 53151

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HXB2 Location	Gag(176-184) p24(44-52) DNA(1315..1341)	Gag Epitope Map
Author Location	p24(176-184)
Epitope	`SEGATPQDL`	Epitope Alignment
Epitope Name	Gag/p24-SL9
Subtype	B
Species (MHC/HLA)	human(B60)
Immunogen	HIV-1 infection
Experimental methods	Chromium-release assay
Keywords	binding affinity, TCR usage, characterizing CD8+ T cells

Notes

Killing efficiency of CTLs and their ability to suppress viral replication is shown to depend more on epitope specificity than to antigenic avidity. Different clones recognizing the same epitope had similar killing efficiency despite their variation in avidity. Nef specific CTL clones tended to be most inhibitory, followed by Gag, then by RT specific clones, regardless of avidity.
1/14 CTL T-cell clones tested were specific for Gag/p24-SL9. Under conditions of excess peptide (100ug/ml), there was no difference in their lytic potential; all possessed similar effector capacity. Avidity was measured as the sensitizing dose of peptide required for 50% of maximal killing (SD50), which varied from 20 pg/ml to 100 ng/ml, over four orders of magnitude for all 14 epitopes. The SD50 value for Gag/p24-SL9 was 30 pg/ml, it was among the peptides with the highest avidity.

References

Displaying record number 53150

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HXB2 Location	Gag(298-306) p24(166-174) DNA(1681..1707)	Gag Epitope Map
Author Location	p24(166-174)
Epitope	`DRFYKTLRA`	Epitope Alignment
Epitope Name	Gag/p24-DA9
Subtype	B
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Experimental methods	Chromium-release assay
Keywords	binding affinity, TCR usage, characterizing CD8+ T cells

Notes

Killing efficiency of CTLs and their ability to suppress viral replication is shown to depend more on epitope specificity than to antigenic avidity. Different clones recognizing the same epitope had similar killing efficiency despite their variation in avidity. Nef specific CTL clones tended to be most inhibitory, followed by Gag, then by RT specific clones, regardless of avidity.
1/14 CTL T-cell clones tested were specific for Gag/p24-DA9. Under conditions of excess peptide (100ug/ml), there was no difference in their lytic potential; all possessed similar effector capacity. Avidity was measured as the sensitizing dose of peptide required for 50% of maximal killing (SD50), which varied from 20 pg/ml to 100 ng/ml, over four orders of magnitude for all 14 epitopes. The SD50 value for Gag/p24-DA9 was 100,000 pg/ml, it had the lowest avidity of the 14 tested.

References

Displaying record number 53152

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HXB2 Location	Pol(464-472) RT(309-317) DNA(3474..3500)	Pol Epitope Map
Author Location	RT(309-317)
Epitope	`ILKEPVHGV`	Epitope Alignment
Epitope Name	RT-IV9
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods	Chromium-release assay
Keywords	binding affinity, TCR usage, characterizing CD8+ T cells

Notes

Killing efficiency of CTLs and their ability to suppress viral replication is shown to depend more on epitope specificity than to antigenic avidity. Different clones recognizing the same epitope had similar killing efficiency despite their variation in avidity. Nef specific CTL clones tended to be most inhibitory, followed by Gag, then by RT specific clones, regardless of avidity.
2/14 CTL T-cell clones tested were specific for RT/IV9. Under conditions of excess peptide (100ug/ml), there was no difference in their lytic potential; all possessed similar effector capacity. Avidity was measured as the sensitizing dose of peptide required for 50% of maximal killing (SD50), which varied from 20 pg/ml to 100 ng/ml, over four orders of magnitude for all 14 epitopes. The SD50 values for the two RT/IV9 clones were very different, 50 and 20,000 pg/ml.

References

Displaying record number 53153

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HXB2 Location	gp160(584-592) gp41(73-81) DNA(7974..8000)	gp160 Epitope Map
Author Location	gp41(73-81)
Epitope	`ERYLKDQQL`	Epitope Alignment
Epitope Name	Env EL9
Subtype	B
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Experimental methods	Chromium-release assay
Keywords	binding affinity, TCR usage, characterizing CD8+ T cells

Notes

Killing efficiency of CTLs and their ability to suppress viral replication is shown to depend more on epitope specificity than to antigenic avidity. Different clones recognizing the same epitope had similar killing efficiency despite their variation in avidity. Nef specific CTL clones tended to be most inhibitory, followed by Gag, then by RT specific clones, regardless of avidity.
4/14 CTL T-cell clones tested were specific for Env EL9. Under conditions of excess peptide (100ug/ml), there was no difference in their lytic potential; all possessed similar effector capacity. Avidity was measured as the sensitizing dose of peptide required for 50% of maximal killing (SD50), which varied from 20 pg/ml to 100 ng/ml, over four orders of magnitude for all 14 epitopes. The SD50 range for Env EL9 was 5,000 - 60,000 pg/ml.

References

Displaying record number 53154

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HXB2 Location	Nef(92-100) DNA(9070..9096)	Nef Epitope Map
Author Location	Nef(92-100)
Epitope	`KEKGGLEGL`	Epitope Alignment
Epitope Name	Nef KL9
Subtype	B
Species (MHC/HLA)	human(B60)
Immunogen	HIV-1 infection
Experimental methods	Chromium-release assay, Flow cytometric T-cell cytokine assay
Keywords	binding affinity, TCR usage, characterizing CD8+ T cells

Notes

Killing efficiency of CTLs and their ability to suppress viral replication is shown to depend more on epitope specificity than to antigenic avidity. Different clones recognizing the same epitope had similar killing efficiency despite their variation in avidity. Nef specific CTL clones tended to be most inhibitory, followed by Gag, then by RT specific clones, regardless of avidity.
2/14 CTL T-cell clones tested were specific for Nef KL9. Under conditions of excess peptide (100ug/ml), there was no difference in their lytic potential; all possessed similar effector capacity. Avidity was measured as the sensitizing dose of peptide required for 50% of maximal killing (SD50), which varied from 20 pg/ml to 100 ng/ml, over four orders of magnitude for all 14 epitopes. The SD50 range for Nef KL9 was 20-30 pg/ml, both high avidity. These clones were among the most efficient at inhibiting viral replication in the set tested, but because of the general lack of correlation between avidity and viral inhibition efficiency in this study, the authors attribute other reasons to Nefs ability to inhibit viral replication that pertain to presentation like kinetics and expression levels.