Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 52327

HXB2 Location	gp160(311-320) gp120(311-320) DNA(7155..7184)	gp160 Epitope Map
Author Location	gp160 (V3)( IIIB)
Epitope	RGPGRAFVTI	Epitope Alignment
Epitope Name	I10
Species (MHC/HLA)	mouse(H-2Dd)
Immunogen	vaccine
Experimental methods
Keywords	acute/early infection

Vaccine Details

Notes

• During acute infection, high doses of virus result in "clonal exhaustion", a depletion of antigen specific T-cells.
• Recently stimulated CTL from BALB/c mice vaccinated with gp160-vaccinia showed a dose- and time-dependent induction of apoptosis when stimulated with antigenic peptide or H-2Dd/peptide tetramers.
• Restimulated CTL showed an upregulation of CD3-chain phosphorylation in comparison to cells stimulated with target cells, indicative of TCR-mediated apoptosis. Furthermore, apoptosis was inhibited by cyclosporin A and U0126, a mitogen activated kinase inhibitor specific for the ERK1/ERK2 MAPK kinase pathway, and a caspase 3 inhibitor.

References

Takahashi2002 Megumi Takahashi, Eiichi Osono, Yohko Nakagawa, Jian Wang, Jay A. Berzofsky, David H. Margulies, and Hidemi Takahashi. Rapid Induction of Apoptosis in CD8+ HIV-1 Envelope-Specific Murine CTLs by Short Exposure to Antigenic Peptide. J. Immunol., 169(11):6588-6593, 1 Dec 2002. PubMed ID: 12444171. Show all entries for this paper.

Displaying record number 52328

HXB2 Location	gp160(311-320) gp120(311-320) DNA(7155..7184)	gp160 Epitope Map
Author Location	gp160 (V3)( MN)
Epitope	IGPGRAFYAT	Epitope Alignment
Epitope Name	MNT10
Species (MHC/HLA)	mouse(H-2Dd)
Immunogen	vaccine
Experimental methods
Keywords	acute/early infection

Vaccine Details

Notes

• During acute infection, high doses of virus result in "clonal exhaustion", a depletion of antigen specific T-cells.
• Recently stimulated CTL from BALB/c mice vaccinated with gp160-vaccinia showed a dose- and time-dependent induction of apoptosis when stimulated with antigenic peptide or H-2Dd/peptide tetramers.
• Restimulated CTL showed an upregulation of CD3-chain phosphorylation in comparison to cells stimulated with target cells, indicative of TCR-mediated apoptosis. Furthermore, apoptosis was inhibited by cyclosporin A and U0126, a mitogen activated kinase inhibitor specific for the ERK1/ERK2 MAPK kinase pathway, and a caspase 3 inhibitor.

References

Takahashi2002 Megumi Takahashi, Eiichi Osono, Yohko Nakagawa, Jian Wang, Jay A. Berzofsky, David H. Margulies, and Hidemi Takahashi. Rapid Induction of Apoptosis in CD8+ HIV-1 Envelope-Specific Murine CTLs by Short Exposure to Antigenic Peptide. J. Immunol., 169(11):6588-6593, 1 Dec 2002. PubMed ID: 12444171. Show all entries for this paper.