Search CTL/CD8+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 53441

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HXB2 Location	Pol(101-110) Protease(45-54) DNA(2385..2414)	Pol Epitope Map
Author Location	Pol(45-54 IIIB)
Epitope	KMIGGIGGFI	Epitope Alignment
		Show epitope variants
Epitope Name	pol45-54
Subtype	B
Species (MHC/HLA)	humanized mouse(A*02:01)
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining
Keywords	epitope processing, vaccine-induced immune responses, immunodominance, variant cross-reactivity, vaccine antigen design

Vaccine Details

Notes

• When A*0201-C3H/J transgenic mice were immunized with plasmids encoding wild-type gag-pol, codon-optimized (CO) gag-pol, and an expression library vaccine expressing 16 fragments of gag-pol fused with ubiquitin for proteosome targeting (ELI), the ELI vaccine produced up to 10-fold higher CD8 T-cell responses than the other two vaccines. In contrast to the wt and CO vaccines, which tended to augment only immunodominant responses, boosting with the ELI vaccine resulted in many CD8 responses against variant epitopes from different HIV-1 clades, and against drug-resistant variants.
• The drug resistant variant of this epitope, kViVgiggfi, was tested. WT and CO vaccines produced low level CD8+ T-cell responses against the B clade form as well as against drug resistant variant, but the ELI vaccine produced much more intense responses against both the WT and the variant, including after boosting.

References

Singh2004 Rana A K Singh and Michael A Barry. Repertoire and Immunofocusing of CD8 T Cell Responses Generated by HIV-1 gag-pol and Expression Library Immunization Vaccines. J. Immunol., 173(7):4387-4393, 1 Oct 2004. PubMed ID: 15383568. Show all entries for this paper.

Displaying record number 53442

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HXB2 Location	Pol(131-140) Protease(75-84) DNA(2475..2504)	Pol Epitope Map
Author Location	Pol(75-84 IIIB)
Epitope	VLVGPTPVNI	Epitope Alignment
		Show epitope variants
Epitope Name	pol75-84
Subtype	B
Species (MHC/HLA)	humanized mouse(A*02:01)
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining
Keywords	epitope processing, vaccine-induced immune responses, immunodominance, variant cross-reactivity, vaccine antigen design

Vaccine Details

Notes

• When A*0201-C3H/J transgenic mice were immunized with plasmids encoding wild-type gag-pol, codon-optimized (CO) gag-pol, and an expression library vaccine expressing 16 fragments of gag-pol fused with ubiquitin for proteosome targeting (ELI), the ELI vaccine produced up to 10-fold higher CD8 T-cell responses than the other two vaccines. In contrast to the wt and CO vaccines, which tended to augment only immunodominant responses, boosting with the ELI vaccine resulted in many CD8 responses against variant epitopes from different HIV-1 clades, and against drug-resistant variants.
• The drug resistant variant of this epitope,vlvgptpTnV, was tested. WT and CO vaccines produced low level CD8+ T-cell responses against the B clade form as well as against drug resistant variant, but the ELI vaccine produced much more intense responses against both the WT and the variant, including after boosting.

References

Singh2004 Rana A K Singh and Michael A Barry. Repertoire and Immunofocusing of CD8 T Cell Responses Generated by HIV-1 gag-pol and Expression Library Immunization Vaccines. J. Immunol., 173(7):4387-4393, 1 Oct 2004. PubMed ID: 15383568. Show all entries for this paper.

Displaying record number 53440

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HXB2 Location	Pol(188-196) RT(33-41) DNA(2646..2672)	Pol Epitope Map
Author Location	Pol(132-140 IIIB)
Epitope	ALVEICTEM	Epitope Alignment
		Show epitope variants
Epitope Name	pol132-140
Subtype	B
Species (MHC/HLA)	humanized mouse(A*02:01)
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining
Keywords	subtype comparisons, vaccine-induced immune responses, immunodominance, variant cross-reactivity, vaccine antigen design

Vaccine Details

Notes

• When A*0201-C3H/J transgenic mice were immunized with plasmids encoding wild-type gag-pol, codon-optimized (CO) gag-pol, and an expression library vaccine expressing 16 fragments of gag-pol fused with ubiquitin for proteosome targeting (ELI), the ELI vaccine produced up to 10-fold higher CD8 T-cell responses than the other two vaccines. In contrast to the wt and CO vaccines, which tended to augment only immunodominant responses, boosting with the ELI vaccine resulted in many CD8 responses against variant epitopes from different HIV-1 clades, and against drug-resistant variants.
• Different variants of this epitope from different clades were tested. WT and CO vaccines produced low level CD8+ T-cell responses against the B clade form as well as against variants from other clades, but the ELI vaccine produced much more intense responses against the B clade and all variants tested, including after boosting. The variants were: clade A, alTDictem; clade C, atTAicEem; and clade D, alIeicSem.

References

Singh2004 Rana A K Singh and Michael A Barry. Repertoire and Immunofocusing of CD8 T Cell Responses Generated by HIV-1 gag-pol and Expression Library Immunization Vaccines. J. Immunol., 173(7):4387-4393, 1 Oct 2004. PubMed ID: 15383568. Show all entries for this paper.

Displaying record number 53443

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HXB2 Location	Pol(464-472) RT(309-317) DNA(3474..3500)	Pol Epitope Map
Author Location	Pol(468-476 IIIB)
Epitope	ILKEPVHGV	Epitope Alignment
Epitope Name	pol468-476
Subtype	B
Species (MHC/HLA)	humanized mouse(A*02:01)
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining
Keywords	epitope processing, vaccine-induced immune responses, variant cross-reactivity, vaccine antigen design

Vaccine Details

Notes

• When A*0201-C3H/J transgenic mice were immunized with plasmids encoding wild-type gag-pol, codon-optimized (CO) gag-pol, and an expression library vaccine expressing 16 fragments of gag-pol fused with ubiquitin for proteosome targeting (ELI), the ELI vaccine produced up to 10-fold higher CD8 T-cell responses than the other two vaccines. In contrast to the wt and CO vaccines, which tended to augment only immunodominant responses, boosting with the ELI vaccine resulted in many CD8 responses against variant epitopes from different HIV-1 clades, and against drug-resistant variants.
• This epitope was recognized in transgenic mice vaccinated with all three vaccine constructs, but the most intense responses were to the ELI vaccine.

References

Singh2004 Rana A K Singh and Michael A Barry. Repertoire and Immunofocusing of CD8 T Cell Responses Generated by HIV-1 gag-pol and Expression Library Immunization Vaccines. J. Immunol., 173(7):4387-4393, 1 Oct 2004. PubMed ID: 15383568. Show all entries for this paper.