HIV molecular immunology database
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|gp160 Epitope Map|
|Author Location||gp120(318-327 IIIB)|
|Species (MHC/HLA)||mouse(H-2d, H-2p, H-2u)|
|Vaccine strain||B clade IIIB|
Shirai1997 M. Shirai, S. Kozlowski, D. H. Margulies, and J. A. Berzofsky. Degenerate MHC Restriction Reveals the Contribution of Class I MHC Molecules in Determining the Fine Specificity of CTL Recognition of an Immunodominant Determinant of HIV-1 gp160 V3 Loop. J. Immunol., 158:3181-3188, 1997. The novel allogeneic presentation of an immunodominant determinant within the HIV-1 gp160 V3 loop by three different class I MHC molecules to the same CD8+ CTL is used to study the influence of the MHC molecule on the fine specificity of CTL recognition. We previously reported that four distinct class I molecules of H-2d,u,p,q presented the V3 decapeptide P18-I10 (RGPGRAFVTI) to CTL. Surprisingly, we found that H- 2d,u,p cells mutually cross-present the P18-I10 peptide to allogeneic CTL clones of each of the other haplotypes, whereas none of these cross- presents to H-2q CTL, nor do H-2q targets present to CTL of the other haplotypes. Here, we explore the critical amino acid residues for the cross-presentation using 10 variant peptides with single amino acid substitutions. The fine specificity examined using these mutant peptides presented by the same MHC class I molecule showed striking similarity among the CTL of each haplotype, expressing either V beta 8.1 or V beta 14. In contrast, the fine specificity is different between the distinct MHC class I molecules even for the lysis by the same CTL, as shown by reciprocal effects of the same substitutions. Thus, peptide fine specificity of a single TCR is influenced by changes in the class I MHC molecules presenting the Ag. PubMed ID: 9120272. Show all entries for this paper.