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HXB2 Location	gp160(435-443) gp120(435-443) DNA(7527..7553)	gp160 Epitope Map
Author Location	Env( 89.6)
Epitope	`YAPPISGQI`	Epitope Alignment
Epitope Name	p41A
Species (MHC/HLA)	macaque
Immunogen	vaccine
Experimental methods
Keywords

Vaccine Details

Vaccine type	DNA
Vaccine strain	B clade 89.6, SIV
Vaccine component	Env, Gag
Adjuvant	IL-2/Ig

Notes

Different HIV strains were used for different regions: SIVmac239 Gag and HIV-1 89.6P Env
Monkeys that received the DNA vaccines augmented with IL-2/Ig were infected when challenged with pathogenic SHIV-89.6P, but had potent CTL responses, stable CD4+ T-cell counts, preserved virus-specific CD4+ T-cell responses, low to undetectable viral loads, and no evidence of disease or mortality by day 140 after challenge -- monkeys that got a sham vaccine had high viral load, progressed to disease, and were half were dead by day 140.
IL2/Ig consisting of interleukin-2 (IL-2) for immune stimulation, and the Fc portion of immunoglobulin G (IgG) for stability, was delivered either as protein or as DNA -- both enhance the CTL response to vaccination, DNA IL2/Ig giving the most intense response.
Responses to a dominant Mamu A*01 gag epitope SIV Gag p11C (CTPYDINQM) and a subdominant epitope HIV-1 Env p41A (YAPPISGQI) were tracked and had good durability prior to challenge, and the higher the prechallenge peak p11C CTL response, the lower the post-challenge viral load.
No NAb responses were detected in the vaccinated monkeys prior to challenge, and comparable peak NAb titers developed in vaccinated monkeys and control monkeys with preserved CD4+ T-cells.
Shen et al. 2000 is an accompanying commentary.

References

Barouch2000 D. H. Barouch, S. Santra, J. E. Schmitz, M. J. Kuroda, T. M. Fu, W. Wagner, M. Bilska, A. Craiu, X. X. Zheng, G. R. Krivulka, K. Beaudry, M. A. Lifton, C. E. Nickerson, W. L. Trigona, K. Punt, D. C. Freed, L. Guan, S. Dubey, D. Casimiro, A. Simon, M. E. Davies, M. Chastain, T. B. Strom, R. S. Gelman, D. C. Montefiori, M. G. Lewis, E. A. Emini, J. W. Shiver, and N. L. Letvin. Control of viremia and prevention of clinical AIDS in rhesus monkeys by cytokine-augmented DNA vaccination. Science, 290(5491):486-92, 20 Oct 2000. PubMed ID: 11039923. Show all entries for this paper.

Shen2000 X. Shen and R. F. Siliciano. Preventing AIDS but not HIV-1 infection with a DNA vaccine. Science, 290(5491):463-5, 20 Oct 2000. PubMed ID: 11183769. Show all entries for this paper.