Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 1394

HXB2 Location	Gag(77-85) p17(77-85) DNA(1018..1044)	Gag Epitope Map
Author Location	Gag(77-85)
Epitope	SLYNTVATL	Epitope Alignment
Species (MHC/HLA)	human(A*0201)
Immunogen
Experimental methods
Keywords	binding affinity

Notes

• This epitope served as a positive control in a study comparing peptide binding affinity to HLA-A201 to CTL responses upon vaccination with a nef DNA vaccine.

References

Sandberg2000 J. K. Sandberg, A. C. Leandersson, C. Devito, B. Kohleisen, V. Erfle, A. Achour, M. Levi, S. Schwartz, K. Karre, B. Wahren, and J. Hinkula. Human immunodeficiency virus type 1 Nef epitopes recognized in HLA-A2 transgenic mice in response to DNA and peptide immunization. Virology, 273(1):112-9, 20 Jul 2000. PubMed ID: 10891413. Show all entries for this paper.

Displaying record number 1396

HXB2 Location	Nef(42-50) DNA(8920..8946)	Nef Epitope Map
Author Location	Nef(44-52 HXB3)
Epitope	ALTSSNTAA	Epitope Alignment
Species (MHC/HLA)	mouse(A*0201)
Immunogen	vaccine
Experimental methods
Keywords	binding affinity, computational epitope prediction

Vaccine Details

Notes

• Ten Nef 9-mer peptides were predicted to have a strong binding affinity with HLA-A*0201 -- of these, four did bind strongly by a T2 class I stabilization assay, several others bound weakly.
• A CTL immune response to only 3/10 peptides was detected by a 51Cr-release assay after immunization of HLA-A201 transgenic mice with either nef DNA under the control of a CMV promotor, coated on gold particles delivered to abdominal skin by gene gun.
• ALTSSNTAA was also tested by subcutaneous injection of Nef peptides in Freund's adjuvant.
• ALTSSNTAA bound weakly to HLA-A2, but it had the strongest CTL response among the three elicited by the DNA vaccine and a strong response to the peptide vaccination.

References

Sandberg2000 J. K. Sandberg, A. C. Leandersson, C. Devito, B. Kohleisen, V. Erfle, A. Achour, M. Levi, S. Schwartz, K. Karre, B. Wahren, and J. Hinkula. Human immunodeficiency virus type 1 Nef epitopes recognized in HLA-A2 transgenic mice in response to DNA and peptide immunization. Virology, 273(1):112-9, 20 Jul 2000. PubMed ID: 10891413. Show all entries for this paper.

Displaying record number 1397

HXB2 Location	Nef(79-87) DNA(9031..9057)	Nef Epitope Map
Author Location	Nef(81-89 HXB3)
Epitope	MTYKAALDL	Epitope Alignment
Species (MHC/HLA)	mouse(A*0201)
Immunogen	vaccine
Experimental methods
Keywords	binding affinity, computational epitope prediction

Vaccine Details

Notes

• Ten Nef 9-mer peptides were predicted to have a strong binding affinity with HLA-A*0201 -- of these, four did bind strongly by a T2 class I stabilization assay, several others bound weakly.
• A CTL immune response to only 3/10 peptides was detected by a 51Cr-release assay after immunization of HLA-A201 transgenic mice with either nef DNA under the control of a CMV promotor coated on, gold particles delivered to abdominal skin by gene gun.
• MTYKAALDL bound weakly to HLA-A2, but the DNA nef vaccine elicited a good CTL response.

References

Sandberg2000 J. K. Sandberg, A. C. Leandersson, C. Devito, B. Kohleisen, V. Erfle, A. Achour, M. Levi, S. Schwartz, K. Karre, B. Wahren, and J. Hinkula. Human immunodeficiency virus type 1 Nef epitopes recognized in HLA-A2 transgenic mice in response to DNA and peptide immunization. Virology, 273(1):112-9, 20 Jul 2000. PubMed ID: 10891413. Show all entries for this paper.

Displaying record number 1395

HXB2 Location	Nef(83-91) DNA(9043..9069)	Nef Epitope Map
Author Location	Nef(85-93 HXB3)
Epitope	AALDLSHFL	Epitope Alignment
Species (MHC/HLA)	mouse(A*0201)
Immunogen	vaccine
Experimental methods
Keywords	binding affinity, computational epitope prediction

Vaccine Details

Notes

• Ten Nef 9-mer peptides were predicted to have strong binding affinity for HLA-A*0201 -- of these, four did bind strongly by a T2 class I stabilization assay, several others bound weakly.
• A CTL immune response to only 3/10 peptides was detected by a 51Cr-release assay after immunization of HLA-A201 transgenic mice with nef DNA under the control of a CMV promotor, coated on gold particles delivered to abdominal skin by gene gun.
• AALDLSHFL was predicted to have a strong binding capacity for HLA-A2, and did, but it was the only one of the peptides recognized that was a strong binder, the other two recognized peptides were weak binders.
• AALDLSHFL was also tested by subcutaneous injection of Nef peptides in Freund's adjuvant and gave a strong response to the peptide.

References

Sandberg2000 J. K. Sandberg, A. C. Leandersson, C. Devito, B. Kohleisen, V. Erfle, A. Achour, M. Levi, S. Schwartz, K. Karre, B. Wahren, and J. Hinkula. Human immunodeficiency virus type 1 Nef epitopes recognized in HLA-A2 transgenic mice in response to DNA and peptide immunization. Virology, 273(1):112-9, 20 Jul 2000. PubMed ID: 10891413. Show all entries for this paper.

Displaying record number 1398

HXB2 Location	Nef(137-145) DNA(9205..9231)	Nef Epitope Map
Author Location	Nef(139-147 HXB3)
Epitope	LTFGWCFKL	Epitope Alignment
Species (MHC/HLA)	mouse(A*0201)
Immunogen	vaccine
Experimental methods
Keywords	binding affinity, computational epitope prediction

Vaccine Details

Notes

• Ten Nef 9-mer peptides were predicted to have a strong binding affinity with HLA-A*0201 -- of these, four did bind strongly by a T2 class I stabilization assay, several others bound weakly.
• A CTL immune response to only 3/10 peptides was detected by a 51Cr-release assay after immunization of HLA-A201 transgenic mice with either nef DNA under the control of a CMV promotor, coated on gold particles delivered to abdominal skin by gene gun -- LTFGWCFKL did not elicit a CTL response.
• LTFGWCFKL was also tested by subcutaneous injection of Nef peptides in Freund's adjuvant, because it bound strongly to HLA-A*0201, and the peptide vaccination did elicit a response.
• The lack of response to the nef DNA vaccine and the response to the peptide suggests LTFGWCFKL may not be processed.

References

Sandberg2000 J. K. Sandberg, A. C. Leandersson, C. Devito, B. Kohleisen, V. Erfle, A. Achour, M. Levi, S. Schwartz, K. Karre, B. Wahren, and J. Hinkula. Human immunodeficiency virus type 1 Nef epitopes recognized in HLA-A2 transgenic mice in response to DNA and peptide immunization. Virology, 273(1):112-9, 20 Jul 2000. PubMed ID: 10891413. Show all entries for this paper.