Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 53137

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HXB2 Location	Pol(90-98) Protease(34-42) DNA(2352..2378)	Pol Epitope Map
Author Location	Protease
Epitope	EEMNLPGRW	Epitope Alignment
Epitope Name	EW9
Species (MHC/HLA)	human(B44)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	epitope processing, supervised treatment interruptions (STI), immunodominance

Notes

• Protease and integrase are shown to be frequently targeted by CD8 T-cell responses (23% and 68% of 56 HIV+ patients, respectively). Responses tend to cluster in conserved regions of Int, although 1 high conserved region had no responses. CTL frequencies per unit protein length for Pro and Int were similar to other HIV non-structural proteins. Three novel HLA class I-restricted optimal epitopes were found and characterized with fine mapping.
• The epitope includes residue M36, which is a known accessory mutation site in individuals treated with PIs.

References

Rodriguez2004 William R. Rodriguez, Marylyn M. Addo, Almas Rathod, Cecily A. Fitzpatrick, Xu G. Yu, Beth Perkins, Eric S. Rosenberg, Marcus Altfeld, and Bruce D. Walker. CD8+ T Lymphocyte Responses Target Functionally Important Regions of Protease and Integrase in HIV-1 Infected Subjects. J. Transl. Med., 2(1):15, 21 May 2004. PubMed ID: 15154967. Show all entries for this paper.

Displaying record number 53139

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HXB2 Location	Pol(838-847) Integrase(123-132) DNA(4596..4625)	Pol Epitope Map
Author Location	Integrase
Epitope	STTVKAACWW	Epitope Alignment
Epitope Name	SW10
Species (MHC/HLA)	human(B57)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	epitope processing, supervised treatment interruptions (STI), rate of progression, immunodominance

Notes

• Protease and integrase are shown to be frequently targeted by CD8 T-cell responses (23% and 68% of 56 HIV+ patients, respectively). Responses tend to cluster in conserved regions of Int, although 1 high conserved region had no responses. CTL frequencies per unit protein length for Pro and Int were similar to other HIV non-structural proteins. Three novel HLA class I-restricted optimal epitopes were found and characterized with fine mapping.
• All 5 HLA-B57 patients recognized this epitope and were long-term nonprogressors.

References

Rodriguez2004 William R. Rodriguez, Marylyn M. Addo, Almas Rathod, Cecily A. Fitzpatrick, Xu G. Yu, Beth Perkins, Eric S. Rosenberg, Marcus Altfeld, and Bruce D. Walker. CD8+ T Lymphocyte Responses Target Functionally Important Regions of Protease and Integrase in HIV-1 Infected Subjects. J. Transl. Med., 2(1):15, 21 May 2004. PubMed ID: 15154967. Show all entries for this paper.

Displaying record number 53138

Download this epitope record as JSON.

HXB2 Location	Pol(934-942) Integrase(219-227) DNA(4884..4910)	Pol Epitope Map
Author Location	Integrase
Epitope	KIQNFRVYY	Epitope Alignment
Epitope Name	KY9
Species (MHC/HLA)	human(A30)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	epitope processing, supervised treatment interruptions (STI), immunodominance

Notes

• Protease and integrase are shown to be frequently targeted by CD8 T-cell responses (23% and 68% of 56 HIV+ patients, respectively). Responses tend to cluster in conserved regions of Int, although 1 high conserved region had no responses. CTL frequencies per unit protein length for Pro and Int were similar to other HIV non-structural proteins. Three novel HLA class I-restricted optimal epitopes were found and characterized with fine mapping.

References

Rodriguez2004 William R. Rodriguez, Marylyn M. Addo, Almas Rathod, Cecily A. Fitzpatrick, Xu G. Yu, Beth Perkins, Eric S. Rosenberg, Marcus Altfeld, and Bruce D. Walker. CD8+ T Lymphocyte Responses Target Functionally Important Regions of Protease and Integrase in HIV-1 Infected Subjects. J. Transl. Med., 2(1):15, 21 May 2004. PubMed ID: 15154967. Show all entries for this paper.