Search CTL/CD8+ T-Cell Epitope Database

Found 8 matching records:

Displaying record number 52755

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HXB2 Location	Gag(11-30) p17(11-30) DNA(820..879)	Gag Epitope Map
Author Location	Gag(11-30)
Epitope	`GELDRWEKIRLRPGGKKKYK`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B62)
Immunogen	HIV-1 infection
Patient MHC/HLA	A2, A32, B27, B62
Experimental methods	Chromium-release assay
Keywords	genital and mucosal immunity

Notes

CTL clones from blood, semen, cervix and rectum samples from 12 individuals were compared. CTL clones derived from blood and mucosal samples had similar high lysis efficiency, primarily perforin dependent, and TCRβ VDJ region sequencing revealed cases of expansion of the same clone in different compartments.
CD8+ T cell clones directed at this epitope were derived from blood and semen.

References

Musey2003 L. Musey, Y. Ding, J. Cao, J. Lee, C. Galloway, A. Yuen, K. R. Jerome, and M. J. McElrath. Ontogeny and Specificities of Mucosal and Blood Human Immunodeficiency Virus Type 1-Specific CD8+ Cytotoxic T Lymphocytes. J. Virol., 77(1):291-300, Jan 2003. PubMed ID: 12477834. Show all entries for this paper.

Displaying record number 52753

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HXB2 Location	Gag(147-155) p24(15-23) DNA(1228..1254)	Gag Epitope Map
Author Location	Gag(147-155)
Epitope	`ISPRTLNAW`	Epitope Alignment
Species (MHC/HLA)	human(B57)
Immunogen	HIV-1 infection
Patient MHC/HLA	A28, A3, B53, B57; A31, B57, B7
Experimental methods	Chromium-release assay
Keywords	TCR usage, genital and mucosal immunity

Notes

CTL clones from blood, semen, cervix and rectum samples from 12 individuals were compared. CTL clones derived from blood and mucosal samples had similar high lysis efficiency, primarily perforin dependent, and TCRβ VDJ region sequencing revealed cases of expansion of the same clone in different compartments.
CD8+ T cell clones directed at this epitope were derived from blood and semen of one male subject, and blood and cervix of one female subject.
From the male patient, six clones that recognized this epitope had three different patterns of TCRβ usage: 2 from the blood and 1 from the semen used Vβ6S2DJ2S2; 1 from the blood and 1 from the semen used Vβ6S2DJ1.1; and 1 from the semen used Vβ7S1DJ2.3.
From the female patient, five clones that recognized this epitope had different TCRβ usage. Blood derived clones were Vβ6S7DJ2.7, Vβ6.4DJ2.3, and Vβ6S3DJ2.1. Cervix derived clones were Vβ6S3DJ1.4 and Vβ6S5DJ2.5.

References

Displaying record number 52754

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HXB2 Location	Gag(164-172) p24(32-40) DNA(1279..1305)	Gag Epitope Map
Author Location	Gag(164-172)
Epitope	`FSPEVIPMF`	Epitope Alignment
Species (MHC/HLA)	human(B57)
Immunogen	HIV-1 infection
Patient MHC/HLA	A28, A3, B53, B57
Experimental methods	Chromium-release assay
Keywords	TCR usage, genital and mucosal immunity

Notes

CTL clones from blood, semen, cervix and rectum samples from 12 individuals were compared. CTL clones derived from blood and mucosal samples had similar high lysis efficiency, primarily perforin dependent, and TCRβ VDJ region sequencing revealed cases of expansion of the same clone in different compartments.
CD8+ T cell clones directed at this epitope were derived from blood and semen.
The TCRβ VDJ rearrangement of a CTL clone from the blood was Vβ21S3DJ1.2, and a clone from the semen used Vβ7S1DJ2.3.

References

Displaying record number 52752

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HXB2 Location	Gag(261-280) p24(129-148) DNA(1570..1629)	Gag Epitope Map
Author Location	Gag(261-280)
Epitope	`IYKLWIILGLNKIVRMYSPT`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B27, B62)
Immunogen	HIV-1 infection
Patient MHC/HLA	A3, A31, B27, B38; A24, B27, B62
Experimental methods	Chromium-release assay
Keywords	genital and mucosal immunity

Notes

CTL clones from blood, semen, cervix and rectum samples from 12 individuals were compared. CTL clones derived from blood and mucosal samples had similar high lysis efficiency, primarily perforin dependent, and TCRβ VDJ region sequencing revealed cases of expansion of the same clone in different compartments.
CD8+ T cell clones directed at this epitope were derived from blood and rectum of one individual, and blood and semen of another. Both individuals are HLA-B27 positive, and within the peptide there is a B27 epitope that was recognized in the blood and rectum of the first patient, and in the blood of the second. A HLA-B62 epitope is also recognized in this peptide in the second individual, and the CD8+ T cells clones from both the blood and semen recognized this epitope.

References

Displaying record number 52756

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HXB2 Location	Gag(264-272) p24(132-140) DNA(1579..1605)	Gag Epitope Map
Author Location	Gag(261-280)
Epitope	`RWIILGLNK`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Patient MHC/HLA	A24, A33, B14, B27; A2, A32, B27, B62
Experimental methods	Chromium-release assay
Keywords	genital and mucosal immunity

Notes

CTL clones from blood, semen, cervix and rectum samples from 12 individuals were compared. CTL clones derived from blood and mucosal samples had similar high lysis efficiency, primarily perforin dependent, and TCRβ VDJ region sequencing revealed cases of expansion of the same clone in different compartments.
CD8+ T cell clones that recognize this epitope were derived from both blood and cervix from a woman, and the blood and semen from a man.

References

Displaying record number 52750

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HXB2 Location	Gag(269-277) p24(137-145) DNA(1594..1620)	Gag Epitope Map
Author Location	Gag(269-277)
Epitope	`GLNKIVRMY`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B62)
Immunogen	HIV-1 infection
Patient MHC/HLA	A2, A24, B27, B62
Experimental methods	Chromium-release assay
Keywords	TCR usage, genital and mucosal immunity

Notes

CTL clones from blood, semen, cervix and rectum samples from 12 individuals were compared. CTL clones derived from blood and mucosal samples had similar high lysis efficiency, primarily perforin dependent, and TCRβ VDJ region sequencing revealed cases of expansion of the same clone in different compartments.
CD8+ T cell clones directed at this epitope were derived from blood, rectum and semen.
The TCRβ VDJ rearrangement of the CTL clones was Vβ22S1DJ1.2, demonstrating expansion of CTL clones in all three compartments from the same progenitor cell.

References

Displaying record number 52757

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HXB2 Location	Gag(298-307) p24(166-175) DNA(1681..1710)	Gag Epitope Map
Author Location	Gag(298-307)
Epitope	`DRFYKTRAE`	Epitope Alignment
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Patient MHC/HLA	A24, A33, B14, B27
Experimental methods	Chromium-release assay
Keywords	TCR usage, genital and mucosal immunity

Notes

CTL clones from blood, semen, cervix and rectum samples from 12 individuals were compared. CTL clones derived from blood and mucosal samples had similar high lysis efficiency, primarily perforin dependent, and TCRβ VDJ region sequencing revealed cases of expansion of the same clone in different compartments.
CD8+ T cell clones directed at this epitope were derived from blood and cervix.

References

Displaying record number 52751

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HXB2 Location	Gag(301-320) p24(169-188) DNA(1690..1749)	Gag Epitope Map
Author Location	Gag(301-320)
Epitope	`YKTLRAEQASQEVKNWMTET`	Epitope Alignment
Species (MHC/HLA)	human(B57)
Immunogen	HIV-1 infection
Patient MHC/HLA	A1, A66, B52, B57
Experimental methods	Chromium-release assay
Keywords	TCR usage, genital and mucosal immunity

Notes

CTL clones from blood, semen, cervix and rectum samples from 12 individuals were compared. CTL clones derived from blood and mucosal samples had similar high lysis efficiency, primarily perforin dependent, and TCRβ VDJ region sequencing revealed cases of expansion of the same clone in different compartments.
CD8+ T cell clones directed at this epitope were derived from blood and rectum.