Search CTL/CD8+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 52758

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HXB2 Location	Gag(147-155) p24(15-23) DNA(1228..1254)	Gag Epitope Map
Author Location
Epitope	ISPRTLNAW	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(B*57:01)
Immunogen	HIV-1 infection
Experimental methods	Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	rate of progression, escape

Notes

• cDNA Gag sequences from a set of 17 HLA-B*5701+ progressors and 10 LTNPs were obtained, and the variation in four p24 B*5701 epitopes examined. Sequence variants were more common (p < 0.01) in the epitopes in the progressors (median 3, range 1-7) than LTNPs (median 2, range 0-4).
• In general use of the autologous protein in a target cell did not diminish the overall CD8+ T-cell responses.

References

Migueles2003 Stephen A. Migueles, Alisha C. Laborico, Hiromi Imamichi, W. Lesley Shupert, Cassandra Royce, Mary McLaughlin, Linda Ehler, Julia Metcalf, Shuying Liu, Claire W. Hallahan, and Mark Connors. The Differential Ability of HLA B*5701+ Long-Term Nonprogressors and Progressors to Restrict Human Immunodeficiency Virus Replication Is Not Caused by Loss of Recognition of Autologous Viral gag Sequences. J. Virol., 77(12):6889-6898, Jun 2003. PubMed ID: 12768008. Show all entries for this paper.

Displaying record number 52760

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HXB2 Location	Gag(162-172) p24(30-40) DNA(1273..1305)	Gag Epitope Map
Author Location	Gag(162-172)
Epitope	KAFSPEVIPMF	Epitope Alignment
		Show epitope variants
Epitope Name	KAF11
Subtype	B
Species (MHC/HLA)	human(B*57:01)
Immunogen	HIV-1 infection
Experimental methods	Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	rate of progression, escape

Notes

• cDNA Gag sequences from a set of 17 HLA-B*5701+ progressors and 10 LTNPs were obtained, and the variation in four p24 B*5701 epitopes examined. Sequence variants were more common (p < 0.01) in the epitopes in the progressors (median 3, range 1-7) than LTNPs (median 2, range 0-4).
• In general, use of the autologous protein in a target cell did not diminish the overall CD8+ T-cell responses.
• This epitope tends to be quantitatively immunodominant in B57+ people, including in some of the individuals in this study. It was extremely well conserved in the sequences obtained here, despite strong immune pressure, suggesting fitness constraints.

References

Migueles2003 Stephen A. Migueles, Alisha C. Laborico, Hiromi Imamichi, W. Lesley Shupert, Cassandra Royce, Mary McLaughlin, Linda Ehler, Julia Metcalf, Shuying Liu, Claire W. Hallahan, and Mark Connors. The Differential Ability of HLA B*5701+ Long-Term Nonprogressors and Progressors to Restrict Human Immunodeficiency Virus Replication Is Not Caused by Loss of Recognition of Autologous Viral gag Sequences. J. Virol., 77(12):6889-6898, Jun 2003. PubMed ID: 12768008. Show all entries for this paper.

Displaying record number 52759

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HXB2 Location	Gag(240-249) p24(108-117) DNA(1507..1536)	Gag Epitope Map
Author Location
Epitope	TSTLQEQIGN	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(B*57:01)
Immunogen	HIV-1 infection
Experimental methods	Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	rate of progression, escape

Notes

• cDNA Gag sequences from a set of 17 HLA-B*5701+ progressors and 10 LTNPs were obtained, and the variation in four p24 B*5701 epitopes examined. Sequence variants were more common (p < 0.01) in the epitopes in the progressors (median 3, range 1-7) than LTNPs (median 2, range 0-4).
• In general, use of the autologous protein in a target cell did not diminish the overall CD8+ T-cell responses.

References

Migueles2003 Stephen A. Migueles, Alisha C. Laborico, Hiromi Imamichi, W. Lesley Shupert, Cassandra Royce, Mary McLaughlin, Linda Ehler, Julia Metcalf, Shuying Liu, Claire W. Hallahan, and Mark Connors. The Differential Ability of HLA B*5701+ Long-Term Nonprogressors and Progressors to Restrict Human Immunodeficiency Virus Replication Is Not Caused by Loss of Recognition of Autologous Viral gag Sequences. J. Virol., 77(12):6889-6898, Jun 2003. PubMed ID: 12768008. Show all entries for this paper.

Displaying record number 52761

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HXB2 Location	Gag(308-316) p24(176-184) DNA(1711..1737)	Gag Epitope Map
Author Location	Gag(308-316)
Epitope	QASQEVKNW	Epitope Alignment
		Show epitope variants
Epitope Name	QW9
Subtype	B
Species (MHC/HLA)	human(B*57:01)
Immunogen	HIV-1 infection
Experimental methods	Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	rate of progression, escape

Notes

• cDNA Gag sequences from a set of 17 HLA-B*5701+ progressors and 10 LTNPs were obtained, and the variation in four p24 B*5701 epitopes examined. Sequence variants were more common (p < 0.01) in the epitopes in the progressors (median 3, range 1-7) than LTNPs (median 2, range 0-4).
• In general use of the autologous protein in a target cell did not diminish the overall CD8+ T-cell responses.
• The substitution E312D (qasqDvknw) was common in progressors (8/17) and rare in LTNP (1/8) (p = 0.06). qasqDvknw and qasqEvknw peptides were made; this mutation does not affect binding to B*57. 2/4 progressors that carried only the D variant could not recognize the D variant peptide, but could recognize the E variant peptide, demonstrating immune escape.

References

Migueles2003 Stephen A. Migueles, Alisha C. Laborico, Hiromi Imamichi, W. Lesley Shupert, Cassandra Royce, Mary McLaughlin, Linda Ehler, Julia Metcalf, Shuying Liu, Claire W. Hallahan, and Mark Connors. The Differential Ability of HLA B*5701+ Long-Term Nonprogressors and Progressors to Restrict Human Immunodeficiency Virus Replication Is Not Caused by Loss of Recognition of Autologous Viral gag Sequences. J. Virol., 77(12):6889-6898, Jun 2003. PubMed ID: 12768008. Show all entries for this paper.