Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 57688

Download this epitope record as JSON.

HXB2 Location	Gag(162-172) p24(30-40) DNA(1273..1305)	Gag Epitope Map
Author Location
Epitope	KAFSPEVIPMF	Epitope Alignment
Epitope Name	KF11
Subtype	B
Species (MHC/HLA)	human(B57)
Immunogen	HIV-1 infection
Country	Germany, United States
Experimental methods	Flow cytometric T-cell cytokine assay, Tetramer binding
Keywords	memory cells

Notes

• CTL effector-memory differentiation (i.e. presence of CD45RO+/CCR7- or CD45RA+/CCR7- CTL) was studied in relation to 2 surface expression markers, CD45RO+/CCR7- (TemRA) and CD57. HIV-1-specific CTL with TemRA expression form an under-represented population of total TemRA+ CTL; but in subjects with slow progression to disease, a large proportion of their HIV-1-specific CTL are TemRA+. This shows HIV-1-specific CTL differentiation to be TCR-dependent with respect to the marker TemRA. For CD57+ CTL however, maturation was TCR-independent since senescence marker CD57 surface expression was similar for (i) different clonotypes targeting the same HIV-1 epitope (ii) epitope-specific CTL clonotypes at varied stages of differentiation. Thus both TCR-dependent and -independent development of CTL are important for their effector-memory repertoire.
• TCRB clonotype distribution between TemRA and TemRO phenotypes of CTL suggest that differentiation is simultaneous from naive CTL to either TemRA or TemRO CTL compartments. This study was conducted on epitope KF11-specific CTL in HLA-B57 subject 10076.
• Differentiation into the TemRA memory phenotype was shown to be selective and variable, driven by the selective expansion of distinct TCR clonotype populations within HIV-1-specific CTL populations. FL8-specific- and KK10-specific-memory-CTL in subject 10022 are composed of varying percentages of different TRBV expressions. FL8-specific memory CTL in subject 10071 also express 3 different TRBV genes, resulting in differing proportions of 3 different memory CTL sub-populations.
• HLA-B57 restricted CTL specific against epitope KF11 were studied by Tetramer staining and flow cytometry. TemRO, TemRA and memory cells against this epitope were followed.
• Other epitopes to which CTL were detected were B15-TY11, B15-GY10, B57-QW9, B15-QW9, A3-RK9 and B8-EI8.

References

Meyer-Olson2010 Dirk Meyer-Olson, Brenna C. Simons, Joseph A. Conrad, Rita M. Smith, Louise Barnett, Shelly L. Lorey, Coley B. Duncan, Ramesh Ramalingam, and Spyros A. Kalams. Clonal Expansion and TCR-Independent Differentiation Shape the HIV-Specific CD8+ Effector-Memory T-Cell Repertoire In Vivo. Blood, 116(3):396-405, Jul 22 2010. PubMed ID: 20424187. Show all entries for this paper.

Displaying record number 57690

Download this epitope record as JSON.

HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location
Epitope	KRWIILGLNK	Epitope Alignment
Epitope Name	KK10
Subtype	B
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Country	Germany, United States
Experimental methods	Flow cytometric T-cell cytokine assay, Tetramer binding
Keywords	memory cells

Notes

• CTL effector-memory differentiation (i.e. presence of CD45RO+/CCR7- or CD45RA+/CCR7- CTL) was studied in relation to 2 surface expression markers, CD45RO+/CCR7- (TemRA) and CD57. HIV-1-specific CTL with TemRA expression form an under-represented population of total TemRA+ CTL; but in subjects with slow progression to disease, a large proportion of their HIV-1-specific CTL are TemRA+. This shows HIV-1-specific CTL differentiation to be TCR-dependent with respect to the marker TemRA. For CD57+ CTL however, maturation was TCR-independent since senescence marker CD57 surface expression was similar for (i) different clonotypes targeting the same HIV-1 epitope (ii) epitope-specific CTL clonotypes at varied stages of differentiation. Thus both TCR-dependent and -independent development of CTL are important for their effector-memory repertoire.
• TCRB clonotype distribution between TemRA and TemRO phenotypes of CTL suggest that differentiation is simultaneous from naive CTL to either TemRA or TemRO CTL compartments. This study was conducted on epitope KF11-specific CTL in HLA-B57 subject 10076.
• Differentiation into the TemRA memory phenotype was shown to be selective and variable, driven by the selective expansion of distinct TCR clonotype populations within HIV-1-specific CTL populations. FL8-specific- and KK10-specific-memory-CTL in subject 10022 are composed of varying percentages of different TRBV expressions. FL8-specific memory CTL in subject 10071 also express 3 different TRBV genes, resulting in differing proportions of 3 different memory CTL sub-populations.
• HLA-B27 restricted CTL specific against epitope KK10 were studied by Tetramer staining and flow cytometry. TemRO, TemRA and memory cells against this epitope were followed.

References

Meyer-Olson2010 Dirk Meyer-Olson, Brenna C. Simons, Joseph A. Conrad, Rita M. Smith, Louise Barnett, Shelly L. Lorey, Coley B. Duncan, Ramesh Ramalingam, and Spyros A. Kalams. Clonal Expansion and TCR-Independent Differentiation Shape the HIV-Specific CD8+ Effector-Memory T-Cell Repertoire In Vivo. Blood, 116(3):396-405, Jul 22 2010. PubMed ID: 20424187. Show all entries for this paper.

Displaying record number 57689

Download this epitope record as JSON.

HXB2 Location	Nef(90-97) DNA(9064..9087)	Nef Epitope Map
Author Location
Epitope	FLKEKGGL	Epitope Alignment
Epitope Name	FL8
Subtype	B
Species (MHC/HLA)	human(B8)
Immunogen	HIV-1 infection
Country	Germany, United States
Experimental methods	Flow cytometric T-cell cytokine assay, Tetramer binding
Keywords	memory cells

Notes

• CTL effector-memory differentiation (i.e. presence of CD45RO+/CCR7- or CD45RA+/CCR7- CTL) was studied in relation to 2 surface expression markers, CD45RO+/CCR7- (TemRA) and CD57. HIV-1-specific CTL with TemRA expression form an under-represented population of total TemRA+ CTL; but in subjects with slow progression to disease, a large proportion of their HIV-1-specific CTL are TemRA+. This shows HIV-1-specific CTL differentiation to be TCR-dependent with respect to the marker TemRA. For CD57+ CTL however, maturation was TCR-independent since senescence marker CD57 surface expression was similar for (i) different clonotypes targeting the same HIV-1 epitope (ii) epitope-specific CTL clonotypes at varied stages of differentiation. Thus both TCR-dependent and -independent development of CTL are important for their effector-memory repertoire.
• TCRB clonotype distribution between TemRA and TemRO phenotypes of CTL suggest that differentiation is simultaneous from naive CTL to either TemRA or TemRO CTL compartments. This study was conducted on epitope KF11-specific CTL in HLA-B57 subject 10076.
• Differentiation into the TemRA memory phenotype was shown to be selective and variable, driven by the selective expansion of distinct TCR clonotype populations within HIV-1-specific CTL populations. FL8-specific- and KK10-specific-memory-CTL in subject 10022 are composed of varying percentages of different TRBV expressions. FL8-specific memory CTL in subject 10071 also express 3 different TRBV genes, resulting in differing proportions of 3 different memory CTL sub-populations.
• HLA-B8 restricted CTL specific against epitope FL8 were studied by Tetramer staining and flow cytometry. TemRO, TemRA and memory cells against this epitope were followed.

References

Meyer-Olson2010 Dirk Meyer-Olson, Brenna C. Simons, Joseph A. Conrad, Rita M. Smith, Louise Barnett, Shelly L. Lorey, Coley B. Duncan, Ramesh Ramalingam, and Spyros A. Kalams. Clonal Expansion and TCR-Independent Differentiation Shape the HIV-Specific CD8+ Effector-Memory T-Cell Repertoire In Vivo. Blood, 116(3):396-405, Jul 22 2010. PubMed ID: 20424187. Show all entries for this paper.