Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 58875

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HXB2 Location	Pol(131-140) Protease(75-84) DNA(2475..2504)	Pol Epitope Map
Author Location	Protease(75-84 MN)
Epitope	VLVGPTPVNI	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(A*02:01)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay, HLA binding, Other
Keywords	binding affinity

Notes

• Peptide predicted to be reactive based on HLA-A*0201 binding motif.
• Peptide could stimulate CTL in PBMC from seronegative donors.
• Peptide located in a highly conserved region of protease.
• Both 9-mer and 10-mer could stimulate CTL: VLVGPTPVNI and LVGPTPVNI.
• Binding affinity to A*0201 was measured, C_{1/2 max}µM = 6 for 10-mer, 3 for 9-mer.
• MAL variant of Pr(75-84 MN), with substitutions V77, G78, and P79, gave reduced binding and CTL recognition.

References

Konya1997 J. Konya, G. Stuber, A. Bjorndal, E. M. Fenyo, and J. Dillner. Primary induction of human cytotoxic lymphocytes against a synthetic peptide of the human immunodeficiency virus type 1 protease. J. Gen. Virol., 78:2217-2224, 1997. PubMed ID: 9292009. Show all entries for this paper.

Displaying record number 400

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HXB2 Location	Pol(132-140) Protease(76-84) DNA(2478..2504)	Pol Epitope Map
Author Location	Protease(75-84 MN)
Epitope	LVGPTPVNI	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*02:01)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay, HLA binding, Other
Keywords	binding affinity

Notes

• Peptide predicted to be reactive based on HLA-A*0201 binding motif.
• Peptide could stimulate CTL in PBMC from 5/6 seronegative donors.
• Peptide located in a highly conserved region of protease.
• Both 9-mer and 10-mer could stimulate CTL: VLVGPTPVNI and LVGPTPVNI.
• Binding affinity to A*0201 was measured, C_{1/2 max}µM = 6 for 10-mer, 3 for 9-mer.
• MAL variant of Pr(75-84 MN), with substitutions V77, G78, and P79, gave reduced binding and CTL recognition.

References

Konya1997 J. Konya, G. Stuber, A. Bjorndal, E. M. Fenyo, and J. Dillner. Primary induction of human cytotoxic lymphocytes against a synthetic peptide of the human immunodeficiency virus type 1 protease. J. Gen. Virol., 78:2217-2224, 1997. PubMed ID: 9292009. Show all entries for this paper.

Displaying record number 516

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HXB2 Location	Pol(464-472) RT(309-317) DNA(3474..3500)	Pol Epitope Map
Author Location	RT(476-484)
Epitope	ILKEPVHGV	Epitope Alignment
Species (MHC/HLA)	human(A*02:01)
Immunogen	in vitro stimulation or selection
Experimental methods
Keywords	binding affinity

Notes

• This epitope was included as a positive control.
• Binding affinity to A*0201 was measured, C_{1/2 max} µM =12.

References

Konya1997 J. Konya, G. Stuber, A. Bjorndal, E. M. Fenyo, and J. Dillner. Primary induction of human cytotoxic lymphocytes against a synthetic peptide of the human immunodeficiency virus type 1 protease. J. Gen. Virol., 78:2217-2224, 1997. PubMed ID: 9292009. Show all entries for this paper.

Menendez-Arias1998 L. Menendez-Arias, A. Mas, and E. Domingo. Cytotoxic T-Lymphocyte Responses to HIV-1 Reverse Transcriptase. Viral Immunol., 11:167-181, 1998. PubMed ID: 10189185. Show all entries for this paper.