Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 57644

HXB2 Location	Gag(349-359) p24(217-227) DNA(1834..1866)	Gag Epitope Map
Author Location	Gag(349-)
Epitope	ACQGVGGPGHK	Epitope Alignment
Epitope Name	Gag349
Subtype	B
Species (MHC/HLA)	human(A*1101)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	HLA binding, Intracellular cytokine staining, Relative replication capacity assay, Tetramer binding
Keywords	rate of progression, immunodominance

Notes

• No correlation was found between ability of A*1101-restricted CTLs recognizing immunodominant epitopes to suppress replication in vitro and the appearance of escape mutants. Results suggest that escape mutations are selected by HIV-1-specific CTLs that have a stronger ability to recognize HIV-1 in vivo but not in vitro.
• 12/16 A*1101-positive individuals had CD8+ T cells specific for this epitope.

References

Koizumi2010 Hirokazu Koizumi, Masao Hashimoto, Mamoru Fujiwara, Hayato Murakoshi, Takayuki Chikata, Mohamed Ali Borghan, Atsuko Hachiya, Yuka Kawashima, Hiroshi Takata, Takamasa Ueno, Shinichi Oka, and Masafumi Takiguchi. Different In Vivo Effects of HIV-1 Immunodominant Epitope-Specific Cytotoxic T Lymphocytes on Selection of Escape Mutant Viruses. J. Virol., 84(11):5508-5519, Jun 2010. PubMed ID: 20335254. Show all entries for this paper.

Displaying record number 57645

HXB2 Location	Pol(675-683) RT(520-528) DNA(4107..4133)	Pol Epitope Map
Author Location	Pol(675-)
Epitope	QIIEQLIKK	Epitope Alignment
Epitope Name	Pol675
Subtype	B
Species (MHC/HLA)	human(A*1101)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	HLA binding, Intracellular cytokine staining, Relative replication capacity assay, Tetramer binding
Keywords	rate of progression

Notes

• No correlation was found between ability of A*1101-restricted CTLs recognizing immunodominant epitopes to suppress replication in vitro and the appearance of escape mutants. Results suggest that escape mutations are selected by HIV-1-specific CTLs that have a stronger ability to recognize HIV-1 in vivo but not in vitro.
• 1/8 A*1101-positive individuals had CD8+ T cells specific for this epitope.

References

Koizumi2010 Hirokazu Koizumi, Masao Hashimoto, Mamoru Fujiwara, Hayato Murakoshi, Takayuki Chikata, Mohamed Ali Borghan, Atsuko Hachiya, Yuka Kawashima, Hiroshi Takata, Takamasa Ueno, Shinichi Oka, and Masafumi Takiguchi. Different In Vivo Effects of HIV-1 Immunodominant Epitope-Specific Cytotoxic T Lymphocytes on Selection of Escape Mutant Viruses. J. Virol., 84(11):5508-5519, Jun 2010. PubMed ID: 20335254. Show all entries for this paper.

Displaying record number 57646

HXB2 Location	Nef(73-82) DNA(9013..9042)	Nef Epitope Map
Author Location	Nef(73-)
Epitope	QVPLRPMTYK	Epitope Alignment
Epitope Name	Nef73
Subtype	B
Species (MHC/HLA)	human(A*1101)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding, Intracellular cytokine staining, Relative replication capacity assay, Tetramer binding
Keywords	rate of progression, immunodominance

Notes

• No correlation was found between ability of A*1101-restricted CTLs recognizing immunodominant epitopes to suppress replication in vitro and the appearance of escape mutants. Results suggest that escape mutations are selected by HIV-1-specific CTLs that have a stronger ability to recognize HIV-1 in vivo but not in vitro.
• 13/16 A*1101-positive individuals had CD8+ T cells specific for this epitope.
• Analysis using 7 Nef73-specific CTL clones confirmed the strong ability of the Nef73-specific CTL clones to suppress HIV-1 replication.
• Nef73-specific ones did not select any escape mutant. The 9F mutation was frequently found, but there was no significant difference in the frequency of this mutation between the HLA-A*1101-positive and -negative subjects.

References

Koizumi2010 Hirokazu Koizumi, Masao Hashimoto, Mamoru Fujiwara, Hayato Murakoshi, Takayuki Chikata, Mohamed Ali Borghan, Atsuko Hachiya, Yuka Kawashima, Hiroshi Takata, Takamasa Ueno, Shinichi Oka, and Masafumi Takiguchi. Different In Vivo Effects of HIV-1 Immunodominant Epitope-Specific Cytotoxic T Lymphocytes on Selection of Escape Mutant Viruses. J. Virol., 84(11):5508-5519, Jun 2010. PubMed ID: 20335254. Show all entries for this paper.

Displaying record number 57647

HXB2 Location	Nef(84-92) DNA(9046..9072)	Nef Epitope Map
Author Location	Nef(84-)
Epitope	AVDLSHFLK	Epitope Alignment
		Show epitope variants
Epitope Name	Nef84
Subtype	B
Species (MHC/HLA)	human(A*1101)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding, Intracellular cytokine staining, Relative replication capacity assay, Tetramer binding
Keywords	rate of progression, immunodominance

Notes

• No correlation was found between ability of A*1101-restricted CTLs recognizing immunodominant epitopes to suppress replication in vitro and the appearance of escape mutants. Results suggest that escape mutations are selected by HIV-1-specific CTLs that have a stronger ability to recognize HIV-1 in vivo but not in vitro.
• 11/16 A*1101-positive individuals had CD8+ T cells specific for either AVDLSHFLK or AlDLSHFLK; both sequences are frequently found in clade B.
• Analysis using 3 Nef84-specific CTL clones confirmed the strong ability of the Nef84-specific CTL clones to suppress HIV-1 replication
• 9R mutation was significantly enriched in HLA-A*1101-positive individuals, while mutations at position 2 were significantly enriched in for HLA-A*1101-negative individuals, suggesting that they were not selected by HLA-A*1101-restricted CTLs.
• CTL clones could not recognize cells infected with Nef84-2L9R, confirming 9R to be an escape mutation, but 9R mutation did not influence the level of PD-1 on Nef84-specific CD8+ T cells.

References

Koizumi2010 Hirokazu Koizumi, Masao Hashimoto, Mamoru Fujiwara, Hayato Murakoshi, Takayuki Chikata, Mohamed Ali Borghan, Atsuko Hachiya, Yuka Kawashima, Hiroshi Takata, Takamasa Ueno, Shinichi Oka, and Masafumi Takiguchi. Different In Vivo Effects of HIV-1 Immunodominant Epitope-Specific Cytotoxic T Lymphocytes on Selection of Escape Mutant Viruses. J. Virol., 84(11):5508-5519, Jun 2010. PubMed ID: 20335254. Show all entries for this paper.

Displaying record number 57695

HXB2 Location	Nef(84-92) DNA(9046..9072)	Nef Epitope Map
Author Location	Nef(84-)
Epitope	ALDLSHFLK	Epitope Alignment
		Show epitope variants
Epitope Name	Nef84-2L
Subtype	B
Species (MHC/HLA)	human(A*1101)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding, Intracellular cytokine staining, Relative replication capacity assay, Tetramer binding
Keywords	rate of progression, immunodominance

Notes

• No correlation was found between ability of A*1101-restricted CTLs recognizing immunodominant epitopes to suppress replication in vitro and the appearance of escape mutants. Results suggest that escape mutations are selected by HIV-1-specific CTLs that have a stronger ability to recognize HIV-1 in vivo but not in vitro.
• 11/16 A*1101-positive individuals had CD8+ T cells specific for either AVDLSHFLK or AlDLSHFLK; both sequences are frequently found in clade B.
• Analysis using 3 Nef84-specific CTL clones confirmed the strong ability of the Nef84-specific CTL clones to suppress HIV-1 replication
• 9R mutation was significantly enriched in HLA-A*1101-positive individuals, while mutations at position 2 were significantly enriched in for HLA-A*1101-negative individuals, suggesting that they were not selected by HLA-A*1101-restricted CTLs.
• Nef84-2L-specific CTL clones could not recognize cells infected with Nef84-2L9R, confirming 9R to be an escape mutation, but 9R mutation did not influence the level of PD-1 on Nef84-specific CD8+ T cells.

References

Koizumi2010 Hirokazu Koizumi, Masao Hashimoto, Mamoru Fujiwara, Hayato Murakoshi, Takayuki Chikata, Mohamed Ali Borghan, Atsuko Hachiya, Yuka Kawashima, Hiroshi Takata, Takamasa Ueno, Shinichi Oka, and Masafumi Takiguchi. Different In Vivo Effects of HIV-1 Immunodominant Epitope-Specific Cytotoxic T Lymphocytes on Selection of Escape Mutant Viruses. J. Virol., 84(11):5508-5519, Jun 2010. PubMed ID: 20335254. Show all entries for this paper.