Search CTL/CD8+ T-Cell Epitope Database

Found 56 matching records:

Displaying record number 1652

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HXB2 Location	Gag(18-26) p17(18-26) DNA(841..867)	Gag Epitope Map
Author Location	p17(18-26)
Epitope	`KIRLRPGGK`	Epitope Alignment
Species (MHC/HLA)	human(A3)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

KIRLRPGGK is cross-reactive for A, B, and D clades.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Kaul2001a R. Kaul, T. Dong, F. A. Plummer, J. Kimani, T. Rostron, P. Kiama, E. Njagi, E. Irungu, B. Farah, J. Oyugi, R. Chakraborty, K. S. MacDonald, J. J. Bwayo, A. McMichael, and S. L. Rowland-Jones. CD8(+) lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J. Clin. Invest., 107(10):1303-10, May 2001. URL: http://www.jci.org/cgi/content/full/107/10/1303. PubMed ID: 11375420. Show all entries for this paper.

Displaying record number 1674

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HXB2 Location	Gag(24-31) p17(24-31) DNA(859..882)	Gag Epitope Map
Author Location	p17(24-31)
Epitope	`GGKKKYRL`	Epitope Alignment
Species (MHC/HLA)	human(B8)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1698

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HXB2 Location	Gag(28-36) p17(28-36) DNA(871..897)	Gag Epitope Map
Author Location	p17(728-736 subtype A)
Epitope	`KYRLKHLVW`	Epitope Alignment
Subtype	A
Species (MHC/HLA)	human(Cw4)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-Cw4 women, 2/2 HEPS and 7/11 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in both of the 2/2 HEPS cases and in 3 of the 7/11 HIV-1 infected women.

References

Displaying record number 1646

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HXB2 Location	Gag(71-79) p17(71-79) DNA(1000..1026)	Gag Epitope Map
Author Location	p17(71-79)
Epitope	`GSEELRSLY`	Epitope Alignment
Species (MHC/HLA)	human(A1)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-A1 women, 1/1 HEPS and 3/3 HIV-1 infected women recognized this epitope, and the response was the dominant HLA-A1 response in all cases.

References

Displaying record number 1649

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HXB2 Location	Gag(77-85) p17(77-85) DNA(1018..1044)	Gag Epitope Map
Author Location	p17(77-85)
Epitope	`SLFNTVATL`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	subtype comparisons, HIV exposed persistently seronegative (HEPS), immunodominance

Notes

Variants SL(F/Y)NTVATL are A/B clade specific.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-A2 women, 1/10 HEPS and 22/26 HIV-1 infected women recognized this epitope, likelihood ratio 18.3, p value < 0.003, and ILK(D/E)PVHGV tended to be more reactive in HEPS women, SL(F/Y)NTVATL in infected women.
The dominant response to this HLA allele was to this epitope in the 1/10 HEPS case and in 18 of the 22/26 HIV-1 infected women that responded.
Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.
Subject ML 1250 had an A2 response to ILK(D/E)PVHGV prior to seroconversion, which switched to SL(F/Y)NTVATL post-seroconversion.
Subjects ML 1575 and ML 1592 had no response to SL(F/Y)NTVATL prior to seroconversion, but made responses post-seroconversion.
Subject ML 1760 had an A2 response to ILK(D/E)PVHGV prior to seroconversion, and gained responses to epitopes A2 SL(F/Y)NTVATL and B27 KRWII(L/M)GLNK post-seroconversion.

References

Displaying record number 1659

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HXB2 Location	Gag(84-92) p17(84-92) DNA(1039..1065)	Gag Epitope Map
Author Location	p17(84-92)
Epitope	`TLYCVHQRI`	Epitope Alignment
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1696

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HXB2 Location	Gag(145-152) p24(13-20) DNA(1222..1245)	Gag Epitope Map
Author Location	p24(145-152)
Epitope	`QAISPRTL`	Epitope Alignment
Species (MHC/HLA)	human(Cw3)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1694

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HXB2 Location	Gag(147-155) p24(15-23) DNA(1228..1254)	Gag Epitope Map
Author Location	p24(147-155)
Epitope	`LSPRTLNAW`	Epitope Alignment
Species (MHC/HLA)	human(B57, B58)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

Variants (L/I)SPRTLNAW are specific for the A/B clades.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B57/B58 women, 4/6 HEPS and 14/17 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in 2 of the 4/6 HEPS cases and in 7 of the 14/17 responsive HIV-1 infected women.

References

Displaying record number 57340

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HXB2 Location	Gag(147-155) p24(15-23) DNA(1228..1254)	Gag Epitope Map
Author Location	p24(147-155)
Epitope	`ISPRTLNAW`	Epitope Alignment
Species (MHC/HLA)	human(B57, B58)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

Variants (L/I)SPRTLNAW are specific for the A/B clades.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B57/B58 women, 4/6 HEPS and 14/17 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in 2 of the 4/6 HEPS cases and in 7 of the 14/17 responsive HIV-1 infected women.

References

Displaying record number 1671

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HXB2 Location	Gag(148-156) p24(16-24) DNA(1231..1257)	Gag Epitope Map
Author Location	p24(148-156)
Epitope	`SPRTLNAWV`	Epitope Alignment
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Subject ML 1203 started with CTL responses to A*6802 DTVLEDINL and to B7 FPVTPQVPLR prior to seroconversion, and upon seroconversion acquired additional responses to A*6802 ETAYFILKL which became dominant, B7 TPGPGVIRYPL, B7 IPRRIRQGL, and B7 SPRTLNAWV.

References

Displaying record number 1650

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HXB2 Location	Gag(151-159) p24(19-27) DNA(1240..1266)	Gag Epitope Map
Author Location	p24(150-159)
Epitope	`TLNAWVKVI`	Epitope Alignment
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	subtype comparisons, HIV exposed persistently seronegative (HEPS)

Notes

Variants TLNAWVKV(I/V) are A/B clade specific.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1695

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HXB2 Location	Gag(162-172) p24(30-40) DNA(1273..1305)	Gag Epitope Map
Author Location	p24(153-164)
Epitope	`KAFSPEVIPMF`	Epitope Alignment
Species (MHC/HLA)	human(B57, B58)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B57/B58 women, 4/6 HEPS and 12/17 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in 2 of the 4/6 HEPS cases and in 7 of the 12/17 HIV-1 infected women.

References

Displaying record number 1676

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HXB2 Location	Gag(183-191) p24(51-59) DNA(1336..1362)	Gag Epitope Map
Author Location	p24(183-191)
Epitope	`DLNMMLNIV`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

Variants DLNMMLNIV/DLNTMLNVV are specific for clades A/B.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B14 women, 4/4 HEPS and 3/7 HIV-1 infected women recognized this epitope, likelihood ratio 4.8, p value 0.1, and HEPS women tended to respond to DLNMMLNIV/DLNTMLNVV, while infected women tended to respond to DRF(F/W)KTLRA.
The dominant response to this HLA allele was to this epitope for all 4/4 HEPS cases and in only one of the 3/7 HIV-1 infected women.
Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.
Four epitopes were considered to be ``resistant epitopes'', as they were preferentially reactive in HEPS women and so may confer resistance, and these were found in three different proteins: A2 ILK(D/E)PVHGV in RT, A*6802 DTVLEDINL in Protease, B14 DLN(M/T)LN(I/V)V in p24 and B18 FRDYVDRF(Y/F)K also in p24.

References

Displaying record number 1690

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HXB2 Location	Gag(203-212) p24(71-80) DNA(1396..1425)	Gag Epitope Map
Author Location	p24(203-212)
Epitope	`DTINEEAAEW`	Epitope Alignment
Species (MHC/HLA)	human(B53)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B53 women, 0/2 HEPS and 7/9 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in 4 of the 7/9 responsive HIV-1 infected women.

References

Displaying record number 1693

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HXB2 Location	Gag(240-249) p24(108-117) DNA(1507..1536)	Gag Epitope Map
Author Location	p24(235-243)
Epitope	`TSTLQEQIGW`	Epitope Alignment
Species (MHC/HLA)	human(B57, B58)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

TSTLQEQIGW cross reacts with both A and B clades.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1685

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HXB2 Location	Gag(254-262) p24(122-130) DNA(1549..1575)	Gag Epitope Map
Author Location	p24(260-268)
Epitope	`PPIPVGDIY`	Epitope Alignment
Species (MHC/HLA)	human(B35)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B35 women, 1/3 HEPS and 3/4 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in the 1/3 HEPS case and in the all 3/4 responsive HIV-1 infected women.
Subject ML 857 shifted from a A*6802 DTVLEDINL and B35 (H/N)PDIVIYQY response prior to seroconversion to a B35 PPIPVGDIY and B35 VPLRPMTY response post-seroconversion.

References

Displaying record number 1681

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HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location	p24(263-272)
Epitope	`KRWIILGLNK`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Subject ML 1760 had an A2 response to ILK(D/E)PVHGV prior to seroconversion, and gained responses to epitopes A2 SL(F/Y)NTVATL and B27 KRWII(L/M)GLNK post-seroconversion.

References

Displaying record number 1680

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HXB2 Location	Gag(293-302) p24(161-170) DNA(1666..1695)	Gag Epitope Map
Author Location	p24(293-302)
Epitope	`FRDYVDRFYK`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B18)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

Variants FRDYVDRF(Y/F)K are specific for the B,D/A,C clades.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B18 women, 3/4 HEPS and 1/9 HIV-1 infected women recognized this epitope, likelihood ratio 5.3, p value 0.04, and HEPS women tended to respond to FRDYVDRFY/FK, while infected women tended to respond to YPLTFGWCY/F.
The dominant response to this HLA allele was to this epitope for all 3/4 HEPS cases and for the single HIV-1 infected women that responded to this epitope.
Four epitopes were considered to be ``resistant epitopes'', as they were preferentially reactive in HEPS women and so may confer resistance, and these were found in three different proteins: A2 ILK(D/E)PVHGV in RT, A*6802 DTVLEDINL in Protease, B14 DLNM/TLN(I/V)V in p24 and B18 FRDYVDRF(Y/F)K also in p24.
Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.

References

Displaying record number 1663

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	p24(296-306)
Epitope	`RDYVDRFFKTL`	Epitope Alignment
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-A24 women, 0/4 HEPS and 6/10 HIV-1 infected women recognized this epitope, likelihood ratio 7.2, p value 0.03, and (R)YL(R/K)DQQLL tended to be reactive in HEPS and infected women, RDYVDRFFKTL in infected women only.
The dominant response to this HLA allele was to this epitope in all of the 6/10 HIV-1 infected women.
Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.
Subject ML 1707 started with a CTL response to A*6802 DTVLEDINL prior to seroconversion, and switched to A*6802 ETAYFILKL and A24 RDYVDRFFKTL post-seroconversion.

References

Displaying record number 1675

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HXB2 Location	Gag(298-306) p24(166-174) DNA(1681..1707)	Gag Epitope Map
Author Location	p24(298-306)
Epitope	`DRFFKTLRA`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

Variants DRF(F/W)KTLRA are specific for clades A/B.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B14 women, 0/4 HEPS and 6/7 HIV-1 infected women recognized this epitope, likelihood ratio 14.4, p value 0.004 and HEPS women tended to respond to DLNMMLNIV/DLNTMLNVV, while infected women tended to respond to DRF(F/W)KTLRA.
The dominant response to this HLA allele was to this epitope for all of the 6/7 HIV-1 infected women.
Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.

References

Displaying record number 1678

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	p24(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1691

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HXB2 Location	Gag(308-316) p24(176-184) DNA(1711..1737)	Gag Epitope Map
Author Location	p24(308-316)
Epitope	`QATQEVKNW`	Epitope Alignment
Species (MHC/HLA)	human(B53)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B53 women, 1/2 HEPS and 7/9 HIV-1 infected women recognized this epitope.

References

Displaying record number 1699

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HXB2 Location	Gag(308-316) p24(176-184) DNA(1711..1737)	Gag Epitope Map
Author Location	p24(176-184)
Epitope	`QASGEVKNW`	Epitope Alignment
Species (MHC/HLA)	human(Cw4)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1689

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HXB2 Location	Gag(309-317) p24(177-185) DNA(1714..1740)	Gag Epitope Map
Author Location	p24(177-185)
Epitope	`ATQEVKNWM`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B53)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

Variants A(T/S)QEVKNWM are specific for the A/B clades.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B53 women, 1/2 HEPS and 5/9 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in the 1/2 HEPS case and in only one of the 5/9 HIV-1 infected women.

References

Displaying record number 1692

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HXB2 Location	Gag(312-321) p24(180-189) DNA(1723..1752)	Gag Epitope Map
Author Location	p24(313-322)
Epitope	`EVKNWMTETL`	Epitope Alignment
Species (MHC/HLA)	human(B53)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1667

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HXB2 Location	Pol(59-67) Protease(3-11) DNA(2259..2285)	Pol Epitope Map
Author Location	Pol
Epitope	`ITLWQRPLV`	Epitope Alignment
Species (MHC/HLA)	human(A74)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ITLWQRPLV cross-reacts with clades A, B and D.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1665

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HXB2 Location	Pol(86-94) Protease(30-38) DNA(2340..2366)	Pol Epitope Map
Author Location	Pol(85-93)
Epitope	`DTVLEDINL`	Epitope Alignment
Species (MHC/HLA)	human(A*68:02)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-A*6802 women, 11/12 HEPS and 6/11 HIV-1 infected women recognized this epitope likelihood ratio 4.4, p value 0.08, and HEPS women tended to respond to DTVLEDINL, infected women tended to ETAYFILKL.
The dominant response to this HLA allele was to this epitope in 10 of the 11/12 HEPS cases, but in only 4 of the 6/11 HIV-1 infected women.
Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.
Four epitopes were considered to be ``resistant epitopes'', as they were preferentially reactive in HEPS women and so may confer resistance, and these were found in three different proteins: A2 ILK(D/E)PVHGV in RT, A*6802 DTVLEDINL in Protease, B14 DLN(M/T)LN(I/V)V in p24 and B18 FRDYVDRF(Y/F)K also in p24.
Subject ML 857 shifted from a A*6802 DTVLEDINL and B35 (H/N)PDIVIYQY response prior to seroconversion to a B35 PPIPVGDIY and B35 VPLRPMTY response post-seroconversion, and the loss of the pre-seroconversion response was not due to sequence variation within these epitopes.
Subject ML 1203 started with CTL responses to A*6802 DTVLEDINL and B7 FPVTPQVPLR prior to seroconversion, and upon seroconversion acquired additional responses to A*6802 ETAYFILKL which became dominant, B7 TPGPGV/IRYPL, B7 IPRRIRQGL, and B7 SPRTLNAWV.
Subject ML 1707 started with a CTL response to A*6802 DTVLEDINL prior to seroconversion, and switched to A*6802 ETAYFILKL and A24 RDYVDRFFKTL post-seroconversion, and the loss of the pre-seroconversion response was not due to sequence variation within the epitope.
Subject ML 1830 made no detectable response prior to seroconversion, but responded to A*6802 DTVLEDINL and A*6802 ETAYFILKL post-seroconversion.

References

Displaying record number 1672

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HXB2 Location	Pol(173-181) RT(18-26) DNA(2601..2627)	Pol Epitope Map
Author Location	Pol(171-180)
Epitope	`GPKVKQWPL`	Epitope Alignment
Subtype	A, B, C, D
Species (MHC/HLA)	human(B8)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

GPKVKQWPL is cross-reactive for clades A, B, C, and D.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 57339

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HXB2 Location	Pol(313-321) RT(158-166) DNA(3021..3047)	Pol Epitope Map
Author Location	Pol(325-333)
Epitope	`SIFQSSMTK`	Epitope Alignment
Species (MHC/HLA)	human(A11, A3, A33)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

Variants (S/A)IFQSSMTK are specific for the A/B clades.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-A3 women, 2/2 HEPS and 3/3 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in one of the 2/2 HEPS cases and in one of the 3/3 HIV-1 infected women.

References

Displaying record number 1654

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HXB2 Location	Pol(313-321) RT(158-166) DNA(3021..3047)	Pol Epitope Map
Author Location	Pol(325-333)
Epitope	`AIFQSSMTK`	Epitope Alignment
Species (MHC/HLA)	human(A11, A3, A33)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

Variants (S/A)IFQSSMTK are specific for the A/B clades.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-A3 women, 2/2 HEPS and 3/3 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in one of the 2/2 HEPS cases and in one of the 3/3 HIV-1 infected women.

References

Displaying record number 1684

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location	Pol(342-350)
Epitope	`HPDIVIYQY`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B35)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

Variants (H/N)PDIVIYQY are specific for the A/B clades.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B35 women, 2/3 HEPS and 1/4 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in only one of the 2/3 HEPS cases, and was not to this epitope in the one responsive HIV-1 infected women.
Subject ML 857 shifted from a A*6802 DTVLEDINL and B35 (H/N)PDIVIYQY response prior to seroconversion to a B35 PPIPVGDIY and B35 VPLRPMTY response post-seroconversion and the loss of the pre-seroconversion response was not due to sequence variation within these epitopes.

References

Displaying record number 1686

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HXB2 Location	Pol(448-456) RT(293-301) DNA(3426..3452)	Pol Epitope Map
Author Location	Pol(447-455)
Epitope	`IPLTEEAEL`	Epitope Alignment
Species (MHC/HLA)	human(B51)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1648

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HXB2 Location	Pol(464-472) RT(309-317) DNA(3474..3500)	Pol Epitope Map
Author Location	Pol(476-484)
Epitope	`ILKDPVHGV`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	subtype comparisons, HIV exposed persistently seronegative (HEPS), immunodominance

Notes

Variants ILK(D/E)PVHGV are A/B clade specific.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-A2 women, 7/10 HEPS and 14/26 HIV-1 infected women recognized this epitope, and ILK(D/E)PVHGV tended to be more reactive in HEPS women, SL(F/Y)NTVATL in infected women.
The dominant response to this HLA allele was to this epitope in all 7/10 HEPS cases but in only 5 of the 14/26 HIV-1 infected women.
Four epitopes were considered to be ``resistant epitopes'', as they were preferentially reactive in HEPS women and so may confer resistance, and these were found in three different proteins: A2 ILK(D/E)PVHGV in RT, A*6802 DTVLEDINL in Protease, B14 DLN(M/T)LN(I/V)V in p24 and B18 FRDYVDRF(Y/F)K also in p24.
Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.
Subject ML 1250 had an A2 response to ILK(D/E)PVHGV prior to seroconversion, which switched to SL(F/Y)NTVATL post-seroconversion.
Subject ML 1760 had an A2 response to ILK(D/E)PVHGV prior to seroconversion, and gained responses to epitopes A2 SL(F/Y)NTVATL and B27 KRWII(L/M)GLNK post-seroconversion.

References

Displaying record number 1658

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HXB2 Location	Pol(496-505) RT(341-350) DNA(3570..3599)	Pol Epitope Map
Author Location	Pol(508-516)
Epitope	`IYQEPFKNLK`	Epitope Alignment
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1666

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HXB2 Location	Pol(811-819) Integrase(96-104) DNA(4515..4541)	Pol Epitope Map
Author Location	Pol(744-752)
Epitope	`ETAYFILKL`	Epitope Alignment
Species (MHC/HLA)	human(A*68:02)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

ETAYFILKL cross-reacts with clades A, B and D.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-A*6802 women, 3/12 HEPS and 9/11 HIV-1 infected women recognized this epitope likelihood ratio 7.9, p value 0.01, and HEPS women tended to respond to DTVLEDINL, while infected women to ETAYFILKL.
The dominant response to this HLA allele was to this epitope in 2 of the 3/12 HEPS cases and in all 9/11 HIV-1 infected women that responded to the epitope.
Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.
Subject ML 1203 started with CTL responses to A*6802 DTVLEDINL and to B7 FPVTPQVPLR prior to seroconversion, and upon seroconversion acquired additional responses to A*6802 ETAYFILKL which became dominant, B7 TPGPG(V/I)RYPL, B7 IPRRIRQGL, and B7 SPRTLNAWV.
Subject ML 1707 started with a CTL response to A*6802 DTVLEDINL prior to seroconversion, and switched to A*6802 ETAYFILKL and A24 RDYVDRFFKTL post-seroconversion.
Subject ML 1830 made no detectable response prior to seroconversion, but responded to A*6802 DTVLEDINL and A*6802 ETAYFILKL post-seroconversion.

References

Displaying record number 1647

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HXB2 Location	Rev(55-63) DNA(8465..8491)	Rev Epitope Map
Author Location	Rev(55-63)
Epitope	`ISERILSTY`	Epitope Alignment
Species (MHC/HLA)	human(A1)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1661

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HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	gp120(53-62)
Epitope	`LFCASDAKAY`	Epitope Alignment
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1687

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HXB2 Location	gp160(78-86) gp120(78-86) DNA(6456..6482)	gp160 Epitope Map
Author Location	gp120(77-85)
Epitope	`DPNPQEVVL`	Epitope Alignment
Species (MHC/HLA)	human(B51)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1664

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HXB2 Location	gp160(208-217) gp120(208-217) DNA(6846..6875)	gp160 Epitope Map
Author Location	gp120(263-272)
Epitope	`VSFEPIPIHY`	Epitope Alignment
Species (MHC/HLA)	human(A29)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1697

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HXB2 Location	gp160(375-383) gp120(375-383) DNA(7347..7373)	gp160 Epitope Map
Author Location	gp120(376-383)
Epitope	`SFNCGGEFF`	Epitope Alignment
Species (MHC/HLA)	human(Cw4)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-Cw4 women, 1/2 HEPS and 10/11 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in 6 of the 10/11 responsive HIV-1 infected women, and not in the HEPS case.

References

Displaying record number 1688

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HXB2 Location	gp160(416-424) gp120(416-424) DNA(7470..7496)	gp160 Epitope Map
Author Location	gp120(378-385)
Epitope	`LPCRIKQII`	Epitope Alignment
Species (MHC/HLA)	human(B51)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1677

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HXB2 Location	gp160(584-592) gp41(73-81) DNA(7974..8000)	gp160 Epitope Map
Author Location	gp41(589-597)
Epitope	`ERYLRDQQL`	Epitope Alignment
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1660

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HXB2 Location	gp160(586-593) gp41(75-82) DNA(7980..8003)	gp160 Epitope Map
Author Location	gp41(591-598)
Epitope	`YLRDQQLL`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	subtype comparisons, HIV exposed persistently seronegative (HEPS), immunodominance

Notes

Variants (R)YL(R/K)DQQLL are specific for the A/B clade.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-A24 women, 3/4 HEPS and 10/10 HIV-1 infected women recognized this epitope, and (R)YL(R/K)DQQLL tended to be reactive in HEPS and infected women, RDYVDRFFKTL in infected women only.
The dominant response to this HLA allele was to this epitope in all 3/4 HEPS cases but in only 4 of the 10/10 HIV-1 infected women.
Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.

References

Displaying record number 1673

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HXB2 Location	gp160(586-593) gp41(75-82) DNA(7980..8003)	gp160 Epitope Map
Author Location	gp41(586-593)
Epitope	`YLKDQQLL`	Epitope Alignment
Species (MHC/HLA)	human(B8)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1682

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HXB2 Location	gp160(606-614) gp41(95-103) DNA(8040..8066)	gp160 Epitope Map
Author Location	gp41(606-614)
Epitope	`TAVPWNASW`	Epitope Alignment
Species (MHC/HLA)	human(B35)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1653

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HXB2 Location	gp160(770-780) gp41(259-269) DNA(8532..8564)	gp160 Epitope Map
Author Location	gp41(775-785)
Epitope	`RLRDLLLIVTR`	Epitope Alignment
Species (MHC/HLA)	human(A3)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1668

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HXB2 Location	gp160(843-851) gp41(332-340) DNA(8751..8777)	gp160 Epitope Map
Author Location	gp41(848-856)
Epitope	`IPRRIRQGL`	Epitope Alignment
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

IPRRIRQGL cross-reacts with clades A, B and D.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B7 women, 2/5 HEPS and 5/6 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in 2 of the 5/6 HIV-1 infected women that responded to the epitope, but in neither of the 2/5 HEPS cases.
Subject ML 1203 started with CTL responses to A*6802 DTVLEDINL and to B7 FPVTPQVPLR prior to seroconversion, and upon seroconversion acquired additional responses to A*6802 ETAYFILKL which became dominant, B7 TPGPGV/IRYPL, B7 IPRRIRQGL, and B7 SPRTLNAWV.

References

Displaying record number 1670

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Subject ML 1203 started with CTL responses to A*6802 DTVLEDINL and to B7 FPVTPQVPLR prior to seroconversion, and upon seroconversion acquired additional responses to A*6802 ETAYFILKL which became dominant, B7 TPGPG(V/I)RYPL, B7 IPRRIRQGL, and B7 SPRTLNAWV.

References

Displaying record number 1656

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HXB2 Location	Nef(73-82) DNA(9013..9042)	Nef Epitope Map
Author Location	Nef(73-82)
Epitope	`QVPLRPMTYK`	Epitope Alignment
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1683

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HXB2 Location	Nef(74-81) DNA(9016..9039)	Nef Epitope Map
Author Location	Nef(75-82)
Epitope	`VPLRPMTY`	Epitope Alignment
Species (MHC/HLA)	human(B35)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Subject ML 857 shifted from a A*6802 DTVLEDINL and B35 (H/N)PDIVIYQY response prior to seroconversion to a B35 PPIPVGDIY and B35 VPLRPMTY response post-seroconversion.

References

Displaying record number 1657

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HXB2 Location	Nef(84-92) DNA(9046..9072)	Nef Epitope Map
Author Location	Nef(84-92)
Epitope	`AVDLSHFLK`	Epitope Alignment
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1655

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HXB2 Location	Nef(86-94) DNA(9052..9078)	Nef Epitope Map
Author Location	Nef(86-94)
Epitope	`DLSHFLKEK`	Epitope Alignment
Species (MHC/HLA)	human(A3)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1669

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HXB2 Location	Nef(128-137) DNA(9178..9207)	Nef Epitope Map
Author Location	Nef(128-137)
Epitope	`TPGPGVRYPL`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B7 women, 4/5 HEPS and 5/6 HIV-1 infected women recognized this epitope.
The dominant response to this HLA allele was to this epitope in 3 of the 4/5 HEPS cases and in 2 of the 5/6 HIV-1 infected women.
Subject ML 1203 started with CTL responses to A*6802 DTVLEDINL and to B7 FPVTPQVPLR prior to seroconversion, and upon seroconversion acquired additional responses to A*6802 ETAYFILKL which became dominant, B7 TPGPG(V/I)RYPL, B7 IPRRIRQGL, and B7 SPRTLNAWV.

References

Displaying record number 1679

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HXB2 Location	Nef(135-143) DNA(9199..9225)	Nef Epitope Map
Author Location	Nef(135-143)
Epitope	`YPLTFGWCY`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B18, B49)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), immunodominance

Notes

Variants YPLTFGWC(Y/F) are specific for the B/D clades.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Among HLA-B18 women, 1/4 HEPS and 8/9 HIV-1 infected women recognized this epitope, likelihood ratio 5.3, p value 0.04, and HEPS women tended to respond to FRDYVDRF(Y/F)K, while infected women tended to respond to YPLTFGWC(Y/F)
The dominant response to this HLA allele was to this epitope for the one reactive HEPS case and in all 8/9 HIV-1 infected women.
Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.

References

Displaying record number 1662

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HXB2 Location	Nef(186-194) DNA(9352..9378)	Nef Epitope Map
Author Location	Nef(186-194)
Epitope	`DSRLAFHHM`	Epitope Alignment
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Displaying record number 1651

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HXB2 Location	Nef(190-198) DNA(9364..9390)	Nef Epitope Map
Author Location	Nef(190-198)
Epitope	`ALKHRAYEL`	Epitope Alignment
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	subtype comparisons, HIV exposed persistently seronegative (HEPS)

Notes

Variants ALKHRAYEL and AFHHVAREL are A/B clade specific.
ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.