Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 57936

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location
Epitope	AMQMLKDTI	Epitope Alignment
		Show epitope variants
Epitope Name	G1
Subtype	A
Species (MHC/HLA)	mouse(H-2Kd)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Cytotoxicity assay, HLA binding, Intracellular cytokine staining
Keywords	immunodominance, vaccine antigen design, optimal epitope

Vaccine Details

Notes

• Using a candidate vaccine immunogen (HIVA) immunodominant epitopes were sequentially removed to test whether subdominant responses could be protective. Subdominant epitopes induced robust responses and became immunodominant in the absence of higher-ranked epitopes. Thus vaccines focused on less-variable regions with subdominant epitopes could be effective.
• Disruption of epitopes H and P caused subdominant epitope G1 to become dominant.
• Disruption of epitope G1,AMQMLKDTI, in addition to epitopes H (RGPGRAFVTI) and P (IFQSSMTKI), caused another epitope to become immunodominant. The mutant used to disrupt the anchor residues of G1 (AMefLKDTa) was confirmed to lack binding to the MHCs H-2Dd, H-2Kd and H-2Ld.

References

Im2011 Eung-Jun Im, Jessie P. Hong, Yaowaluck Roshorm, Anne Bridgeman, Sven Létourneau, Peter Liljeström, Mary Jane Potash, David J. Volsky, Andrew J. McMichael, and Tomáš Hanke. Protective Efficacy of Serially Up-Ranked Subdominant CD8+ T Cell Epitopes against Virus Challenges. PLoS Pathog., 7(5):e1002041, May 2011. PubMed ID: 21625575. Show all entries for this paper.

Displaying record number 57938

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HXB2 Location	Gag(239-247) p24(107-115) DNA(1504..1530)	Gag Epitope Map
Author Location
Epitope	TTSTLQEQI	Epitope Alignment
Epitope Name	G2
Subtype	A
Species (MHC/HLA)	mouse(H-2Kd)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Cytotoxicity assay, HLA binding, Intracellular cytokine staining
Keywords	immunodominance, vaccine antigen design, optimal epitope

Vaccine Details

Notes

• Using a candidate vaccine immunogen (HIVA), immunodominant epitopes were sequentially removed to test whether subdominant responses could be protective. Subdominant epitopes induced robust responses and became immunodominant in the absence of higher-ranked epitopes. Thus vaccines focused on less-variable regions with subdominant epitopes could be effective.
• Disruption of dominant epitopes H (RGPGRAFVTI), P (IFQSSMTKI), G1 (AMQMLKDTI), and NPP (NPPIPVGDI) caused subdominant epitope G2, TTSTLQEQI, to become dominant.

References

Im2011 Eung-Jun Im, Jessie P. Hong, Yaowaluck Roshorm, Anne Bridgeman, Sven Létourneau, Peter Liljeström, Mary Jane Potash, David J. Volsky, Andrew J. McMichael, and Tomáš Hanke. Protective Efficacy of Serially Up-Ranked Subdominant CD8+ T Cell Epitopes against Virus Challenges. PLoS Pathog., 7(5):e1002041, May 2011. PubMed ID: 21625575. Show all entries for this paper.

Displaying record number 57937

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HXB2 Location	Gag(253-261) p24(121-129) DNA(1546..1572)	Gag Epitope Map
Author Location
Epitope	NPPIPVGDI	Epitope Alignment
Epitope Name	NPP
Subtype	A
Species (MHC/HLA)	mouse(H-2Dd, H-2Ld)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Cytotoxicity assay, HLA binding, Intracellular cytokine staining
Keywords	immunodominance, vaccine antigen design, optimal epitope

Vaccine Details

Notes

• Using a candidate vaccine immunogen (HIVA), immunodominant epitopes were sequentially removed to test whether subdominant responses could be protective. Subdominant epitopes induced robust responses and became immunodominant in the absence of higher-ranked epitopes. Thus vaccines focused on less-variable regions with subdominant epitopes could be effective.
• Mutation of dominant epitopes H (RGPGRAFVTI), P (IFQSSMTKI), and G1 (AMQMLKDTI) revealed response to peptide NPPIPVGDIYKRWIILGLNK. Peptide NPPIPVGDI was subsequently shown to be restricted by H-2Dd and H-2Ld.

References

Im2011 Eung-Jun Im, Jessie P. Hong, Yaowaluck Roshorm, Anne Bridgeman, Sven Létourneau, Peter Liljeström, Mary Jane Potash, David J. Volsky, Andrew J. McMichael, and Tomáš Hanke. Protective Efficacy of Serially Up-Ranked Subdominant CD8+ T Cell Epitopes against Virus Challenges. PLoS Pathog., 7(5):e1002041, May 2011. PubMed ID: 21625575. Show all entries for this paper.

Displaying record number 57934

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HXB2 Location	Pol(314-322) RT(159-167) DNA(3024..3050)	Pol Epitope Map
Author Location
Epitope	IFQSSMTKI	Epitope Alignment
		Show epitope variants
Epitope Name	P
Subtype	A
Species (MHC/HLA)	mouse(H-2Kd)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Cytotoxicity assay, HLA binding, Intracellular cytokine staining
Keywords	immunodominance, vaccine antigen design, optimal epitope

Vaccine Details

Notes

• Using a candidate vaccine immunogen (HIVA), immunodominant epitopes were sequentially removed to test whether subdominant responses could be protective. Subdominant epitopes induced robust responses and became immunodominant in the absence of higher-ranked epitopes. Thus vaccines focused on less-variable regions with subdominant epitopes could be effective.
• When the immunodominant epitope H (RGPGRAFVTI) was deleted, subdominant epitope P (IFQSSMTKI) became immunodominant.
• Mutation of epitope P to disrupt the anchor residues caused subdominant epitope G1 (AMQMLKDTI) to become dominant. The mutant used to replace epitope P (IFgSSMTKa) was confirmed to lack binding to MHCs H-2D^d, H-2K^d and H-2L^d.
• Fine mapping of this epitope, P, revealed IFQSSMTKI to be the optimal epitope.

References

Im2011 Eung-Jun Im, Jessie P. Hong, Yaowaluck Roshorm, Anne Bridgeman, Sven Létourneau, Peter Liljeström, Mary Jane Potash, David J. Volsky, Andrew J. McMichael, and Tomáš Hanke. Protective Efficacy of Serially Up-Ranked Subdominant CD8+ T Cell Epitopes against Virus Challenges. PLoS Pathog., 7(5):e1002041, May 2011. PubMed ID: 21625575. Show all entries for this paper.

Displaying record number 57935

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HXB2 Location	gp160(311-320) gp120(311-320) DNA(7155..7184)	gp160 Epitope Map
Author Location
Epitope	RGPGRAFVTI	Epitope Alignment
Epitope Name	H
Subtype	A
Species (MHC/HLA)	mouse(H-2Dd, H-2Ld)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Cytotoxicity assay, HLA binding, Intracellular cytokine staining
Keywords	immunodominance, vaccine antigen design, optimal epitope

Vaccine Details

Notes

• Using a candidate vaccine immunogen (HIVA), immunodominant epitopes were sequentially removed to test whether subdominant responses could be protective. Subdominant epitopes induced robust responses and became immunodominant in the absence of higher-ranked epitopes. Thus vaccines focused on less-variable regions with subdominant epitopes could be effective.
• When this immunodominant epitope H (RGPGRAFVTI) was deleted, subdominant epitope P (IFQSSMTKI) became immunodominant.

References

Im2011 Eung-Jun Im, Jessie P. Hong, Yaowaluck Roshorm, Anne Bridgeman, Sven Létourneau, Peter Liljeström, Mary Jane Potash, David J. Volsky, Andrew J. McMichael, and Tomáš Hanke. Protective Efficacy of Serially Up-Ranked Subdominant CD8+ T Cell Epitopes against Virus Challenges. PLoS Pathog., 7(5):e1002041, May 2011. PubMed ID: 21625575. Show all entries for this paper.