Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 1169

Download this epitope record as JSON.

HXB2 Location	Gag(77-85) p17(77-85) DNA(1018..1044)	Gag Epitope Map
Author Location	p17(77-85)
Epitope	SLYNTVATL	Epitope Alignment
Species (MHC/HLA)	human(A*02)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART

Notes

• The single cell ELISPOT assay was optimized and highly specific, and found to work well even after the primary cells had been frozen and thawed.
• Increases in gamma IFN producing cells were observed in response to anti-retroviral therapy using single cell IFN-gamma-production ELISPOT.
• 4/8 A*02 subjects had a positive response to this epitope indicating that it is a major epitope for CD8+ gamma IFN production.
• In 3/3 HLA A*02, B*27 individuals, the dominant response in gag measured by both gamma IFN production and T-cell lysis was a B27 epitope, p24(263-272), not the A2 SLYNTVATL epitope.

References

Huang2000 X. L. Huang, Z. Fan, C. Kalinyak, J. W. Mellors, and C. R. Rinaldo. CD8+ T Cell Gamma Interferon Production Specific for Human Immunodeficiency Virus Type 1 (HIV-1) in HIV-1-Infected Subjects. Clin. Diagn. Lab. Immunol., 7:279-287, 2000. PubMed ID: 10702505. Show all entries for this paper.

Displaying record number 150

Download this epitope record as JSON.

HXB2 Location	Gag(151-159) p24(19-27) DNA(1240..1266)	Gag Epitope Map
Author Location	p24(151-159)
Epitope	TLNAWVKVV	Epitope Alignment
Species (MHC/HLA)	human(A*02)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART, immunodominance

Notes

• The single cell ELISPOT assay was optimized and highly specific, and found to work well even after the primary cells had been frozen and thawed.
• Increases in gamma interferon producing cells were observed in response to anti-retroviral therapy using single cell IFN-gamma-production ELISPOT.
• In 3/3 HLA-A*02, -B*27 subjects the immunodominant epitope was against HLA B*27 Gag p24 epitope KRWIILGL, not A2 Gag epitopes.

References

Huang2000 X. L. Huang, Z. Fan, C. Kalinyak, J. W. Mellors, and C. R. Rinaldo. CD8+ T Cell Gamma Interferon Production Specific for Human Immunodeficiency Virus Type 1 (HIV-1) in HIV-1-Infected Subjects. Clin. Diagn. Lab. Immunol., 7:279-287, 2000. PubMed ID: 10702505. Show all entries for this paper.

Displaying record number 267

Download this epitope record as JSON.

HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location	p24(263-272)
Epitope	KRWIILGLNK	Epitope Alignment
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART

Notes

• The single cell ELISPOT assay was optimized and highly specific, and found to work well even after the primary cells had been frozen and thawed.
• Increases in gamma interferon producing cells were observed in response to anti-retroviral therapy using single cell IFN-gamma-production ELISPOT.
• In 3/3 HLA A*02, B*27 individuals, the dominant response in gag measured by both gamma IFN production and T cell lysis was to the B27 epitope, KRWIILLGLNK, not the A2 SLYNTVATL epitope.

References

Huang2000 X. L. Huang, Z. Fan, C. Kalinyak, J. W. Mellors, and C. R. Rinaldo. CD8+ T Cell Gamma Interferon Production Specific for Human Immunodeficiency Virus Type 1 (HIV-1) in HIV-1-Infected Subjects. Clin. Diagn. Lab. Immunol., 7:279-287, 2000. PubMed ID: 10702505. Show all entries for this paper.

Displaying record number 499

Download this epitope record as JSON.

HXB2 Location	Pol(464-472) RT(309-317) DNA(3474..3500)	Pol Epitope Map
Author Location	RT(476-484)
Epitope	ILKEPVHGV	Epitope Alignment
Species (MHC/HLA)	human(A*02)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART

Notes

• The single cell ELISPOT assay was optimized and highly specific, and found to work well even after the primary cells had been frozen and thawed.
• Increases in gamma interferon producing cells were observed in response to anti-retroviral therapy using single cell IFN-gamma-production ELISPOT.

References

Huang2000 X. L. Huang, Z. Fan, C. Kalinyak, J. W. Mellors, and C. R. Rinaldo. CD8+ T Cell Gamma Interferon Production Specific for Human Immunodeficiency Virus Type 1 (HIV-1) in HIV-1-Infected Subjects. Clin. Diagn. Lab. Immunol., 7:279-287, 2000. PubMed ID: 10702505. Show all entries for this paper.

Displaying record number 676

Download this epitope record as JSON.

HXB2 Location	gp160(192-200) gp120(192-200) DNA(6798..6824)	gp160 Epitope Map
Author Location	gp120(192-199)
Epitope	KLTSCNTSV	Epitope Alignment
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART

Notes

• The single cell ELISPOT assay was optimized and highly specific, and found to work well even after the primary cells had been frozen and thawed.
• Increases in gamma interferon producing cells were observed in response to anti-retroviral therapy using single cell IFN-gamma-production ELISPOT.

References

Huang2000 X. L. Huang, Z. Fan, C. Kalinyak, J. W. Mellors, and C. R. Rinaldo. CD8+ T Cell Gamma Interferon Production Specific for Human Immunodeficiency Virus Type 1 (HIV-1) in HIV-1-Infected Subjects. Clin. Diagn. Lab. Immunol., 7:279-287, 2000. PubMed ID: 10702505. Show all entries for this paper.