Search CTL/CD8+ T-Cell Epitope Database

Found 14 matching records:

Displaying record number 61914

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HXB2 Location	Gag(147-158) p24(15-26) DNA(1228..1263)	Gag Epitope Map
Author Location	Gag(439-474)
Epitope	ISPRTLNAWVKV	Epitope Alignment
Epitope Name	CE1-1
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. The p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE1 comprised ISPRTLNAWVKV as well as CE1-2 LSPRTLNAWVKV in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE1, LSPRTLNAWVKL.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61915

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HXB2 Location	Gag(147-158) p24(15-26) DNA(1228..1263)	Gag Epitope Map
Author Location	Gag(439-474)
Epitope	LSPRTLNAWVKV	Epitope Alignment
Epitope Name	CE1-2
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE1 comprised LSPRTLNAWVKV as well as CE1-1 iSPRTLNAWVKV in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE1, LSPRTLNAWVKL.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61916

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HXB2 Location	Gag(168-185) p24(36-53) DNA(1291..1344)	Gag Epitope Map
Author Location	Gag(502-555)
Epitope	VIPMFSALSEGATPQDLN	Epitope Alignment
Epitope Name	CE2-1
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE2 comprised VIPMFSALSEGATPQDLN as well as CE2-2 VIPMFtALSEGATPQDLN in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, VVPGFQALSEGCTPYDIN and VVPGFQALSEGCAPYDIN.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61917

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HXB2 Location	Gag(168-185) p24(36-53) DNA(1291..1344)	Gag Epitope Map
Author Location	Gag(502-555)
Epitope	VIPMFTALSEGATPQDLN	Epitope Alignment
Epitope Name	CE2-2
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE2 comprised VIPMFTALSEGATPQDLN as well as CE2-1 VIPMFsALSEGATPQDLN in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, VVPGFQALSEGCTPYDIN and VVPGFQALSEGCAPYDIN.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61918

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HXB2 Location	Gag(191-214) p24(59-82) DNA(1360..1431)	Gag Epitope Map
Author Location	Gag(571-642)
Epitope	VGGHQAAMQMLKDTINEEAAEWDR	Epitope Alignment
Epitope Name	CE3-1
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE3 comprised VGGHQAAMQMLKDTINEEAAEWDR as well as CE3-2 VGGHQAAMQMLKeTINEEAAEWDR in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, VGDHQAAMQIIRDIINEEAADWDL and VGEHQAAMQIIRDIINEEAADWDL.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61919

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HXB2 Location	Gag(191-214) p24(59-82) DNA(1360..1431)	Gag Epitope Map
Author Location	Gag(571-642)
Epitope	VGGHQAAMQMLKETINEEAAEWDR	Epitope Alignment
Epitope Name	CE3-2
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE3 comprised VGGHQAAMQMLKETINEEAAEWDR as well as CE3-1 VGGHQAAMQMLKdTINEEAAEWDR in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, VGDHQAAMQIIRDIINEEAADWDL and VGEHQAAMQIIRDIINEEAADWDL.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61920

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HXB2 Location	Gag(231-249) p24(99-117) DNA(1480..1536)	Gag Epitope Map
Author Location	Gag(681-747)
Epitope	PRGSDIAGTTSTLQEQIGW	Epitope Alignment
Epitope Name	CE4-1
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE4 comprised PRGSDIAGTTSTLQEQIGW as well as CE4-2 PRGSDIAGTTSTLQEQIaW in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, PSGSDIAGTTSSVDEQIQW and PRGSDIAGTTSTVEEQIQW.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61921

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HXB2 Location	Gag(231-249) p24(99-117) DNA(1480..1536)	Gag Epitope Map
Author Location	Gag(681-747)
Epitope	PRGSDIAGTTSTLQEQIAW	Epitope Alignment
Epitope Name	CE4-2
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE4 comprised PRGSDIAGTTSTLQEQIAW as well as CE4-1 PRGSDIAGTTSTLQEQIgW in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, PSGSDIAGTTSSVDEQIQW and PRGSDIAGTTSTVEEQIQW.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61922

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HXB2 Location	Gag(263-282) p24(131-150) DNA(1576..1635)	Gag Epitope Map
Author Location	Gag(787-846)
Epitope	KRWIILGLNKIVRMYSPTSI	Epitope Alignment
Epitope Name	CE5-1
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE5 comprised KRWIILGLNKIVRMYSPTSI as well as CE5-2 KRWIILGLNKIVRMYSPvSI in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, RRWIQLGLQKCVRMYNPTNI and RRWIQLGLQKCVRMYNPVNI.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61923

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HXB2 Location	Gag(263-282) p24(131-150) DNA(1576..1635)	Gag Epitope Map
Author Location	Gag(787-846)
Epitope	KRWIILGLNKIVRMYSPVSI	Epitope Alignment
Epitope Name	CE5-2
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE5 comprised KRWIILGLNKIVRMYSPVSI as well as CE5-1 KRWIILGLNKIVRMYSPtSI in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, RRWIQLGLQKCVRMYNPTNI and RRWIQLGLQKCVRMYNPVNI.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61924

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HXB2 Location	Gag(296-309) p24(164-177) DNA(1675..1716)	Gag Epitope Map
Author Location	Gag(886-927)
Epitope	YVDRFYKTLRAEQA	Epitope Alignment
Epitope Name	CE6-1
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE6 comprised YVDRFYKTLRAEQA as well as CE6-2 YVDRFfKTLRAEQA in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, YVDRFYKSLRAEQT.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61925

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HXB2 Location	Gag(296-309) p24(164-177) DNA(1675..1716)	Gag Epitope Map
Author Location	Gag(886-927)
Epitope	YVDRFFKTLRAEQA	Epitope Alignment
Epitope Name	CE6-2
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE6 comprised YVDRFFKTLRAEQA as well as CE6-1 YVDRFyKTLRAEQA in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, YVDRFYKSLRAEQT.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61926

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HXB2 Location	Gag(343-359) p24(211-227) DNA(1816..1866)	Gag Epitope Map
Author Location	Gag(1027-1077)
Epitope	LEEMMTACQGVGGPGHK	Epitope Alignment
Epitope Name	CE7-1
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE7 comprised LEEMMTACQGVGGPGHK as well as CE7-2 LEEMMTACQGVGGPsHK in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, LEEMLTACQGVGGPGQK.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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Displaying record number 61927

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HXB2 Location	Gag(343-359) p24(211-227) DNA(1816..1866)	Gag Epitope Map
Author Location	Gag(1027-1077)
Epitope	LEEMMTACQGVGGPSHK	Epitope Alignment
Epitope Name	CE7-2
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

• To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. the p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
• Conserved elements CE7 comprised LEEMMTACQGVGGPSHK as well as CE7-1 LEEMMTACQGVGGPgHK in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE2, LEEMLTACQGVGGPGQK.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.

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