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Found 2 matching records:

Displaying record number 57920

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HXB2 Location	Pol(328-336) RT(173-181) DNA(3066..3092)	Pol Epitope Map
Author Location	Pol(328-336)
Epitope	`KQNPDIVIY`	Epitope Alignment
Epitope Name	Pol 11-164 or Pol 328-9 or KY9
Subtype	B
Species (MHC/HLA)	human(C*12:02)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Intracellular cytokine staining
Keywords	optimal epitope, novel epitope

Notes

Two novel HLA-Cw*1202-restricted epitopes were identified and tested for the ability to select escape mutants.
Optimal epitope KQNPDIVIY was identified by responses to overlapping peptides.
CTL clones of the epitope were able to kill Cw*1202 cells expressing HIV-1, confirming that the epitope is a naturally processed CTL epitope.

References

Honda2011 Kazutaka Honda, Nan Zheng, Hayato Murakoshi, Masao Hashimoto, Keiko Sakai, Mohamed Ali Borghan, Takayuki Chikata, Madoka Koyanagi, Yoshiko Tamura, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi. Selection of Escape Mutant by HLA-C-Restricted HIV-1 Pol-Specific Cytotoxic T Lymphocytes Carrying Strong Ability to Suppress HIV-1 Replication. Eur. J. Immunol., 41(1):197-106, Jan 2011. PubMed ID: 21182081. Show all entries for this paper.

Displaying record number 57919

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HXB2 Location	Pol(464-473) RT(309-318) DNA(3474..3503)	Pol Epitope Map
Author Location	Pol(463-472)
Epitope	`ILKEPVHGVY`	Epitope Alignment
		Show epitope variants
Epitope Name	Pol 463-10 or IY10
Subtype	B
Species (MHC/HLA)	human(C*12:02)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Intracellular cytokine staining, Relative replication capacity assay
Keywords	escape, optimal epitope, novel epitope

Notes

Two novel HLA-Cw*1202-restricted epitopes were identified and tested for the ability to select escape mutants.
Optimal epitope ILKEPVHGVY was identified by responses to overlapping peptides.
CTL clones of the epitope were able to kill Cw*1202 cells expressing HIV-1, confirming that the epitope is a naturally processed CTL epitope.
The 9A mutant was associated with Cw*1202 absence in a cohort of 108 patients. ILKEPVHGVY-specific clones effectively killed target cells infected with the WT peptide, but failed to kill those infected with 9A mutant, indicating an escape. The mutant also showed up in 4 HLA-Cw*1202 patients late time in infection, suggesting slow selection. Finally, the mutant had a higher fitness cost than WT in a competitive replication assay, and 3/5 Cw*1202- individuals showed the reversion within 2 years after 9A mutant was found.