HIV molecular immunology database

 

Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 113

HXB2 Location Gag(88-115)
p17(88-115)
DNA(1051..1134)
Gag Epitope Map
Author Location p17(88-115 ARV)
Epitope VHQRIEIKDTKEALDKIEEEQNKSKKKA Epitope Alignment
Species (MHC/HLA) mouse(H-2d)
Immunogen vaccine
Experimental methods  
Keywords  

Vaccine Details

Vaccine type peptide
Vaccine component CD4BS, HPG30, Env V3
Adjuvant IL-12

Notes

References

Hamajima1997 K. Hamajima, J. Fukushima, H. Bukawa, T. Kaneko, T. Tsuji, Y. Asakura, S. Sasaki, K. Q. Xin, and K. Okuda. Strong Augment Effect of IL-12 Expression Plasmid on the Induction of HIV-Specific Cytotoxic T Lymphocyte Activity by a Peptide Vaccine Candidate. Clin. Immunol. Immunopathol., 83:179-184, 1997. We previously reported that repeated inoculation of VC1, a macromolecular multicomponent peptide vaccine emulsified with Freund's adjuvant (VC1-F), induced high cytotoxic T lymphocyte (CTL) levels and a substantial level of multivalent antibodies which neutralized various human immunodeficiency virus type 1 (HIV-1) isolates. In the present study, we report that inoculation of VC1-F plus interleukin (IL)-12 expression plasmid can induce a higher antigen-specific CTL response compared to that with VC1-F alone. VC1-F plus IL-12 expression plasmid or VC1-F alone were inoculated to BALB/c mice twice at interval of 2 weeks. Two weeks after the second inoculation, spleen effector cells from these mice were examined. Stronger CTL responses against target cells were observed from the inoculation of VC1-F plus IL-12 plasmid than from that with VC-1F alone, but there was no difference in antibody induction. The inoculation of VC1 plus IL-12 plasmid also produced higher CTL activity than the inoculation of VC1 alone. These augmented CTL activities were not observed using target cells pulsed with non-HIV-specific peptides and different class I haplotype cells. These data demonstrate that co-inoculation of cell-mediated immune potent antigen and IL-12 plasmids can enhance the antigen-specific CTL response. This may be a potential approach for the induction of cellular immunization against HIV-1 and other diseases. PubMed ID: 9143379. Show all entries for this paper.


Displaying record number 757

HXB2 Location gp160(311-320)
gp120(311-320)
DNA(7155..7184)
gp160 Epitope Map
Author Location gp160(318-327 IIIB)
Epitope RGPGRAFVTI Epitope Alignment
RGPGRAFVTI epitope logo
Species (MHC/HLA) mouse(H-2d)
Immunogen vaccine
Experimental methods  
Keywords  

Vaccine Details

Vaccine type peptide
Vaccine component CD4BS, HPG30, Env V3
Adjuvant IL-12

Notes

References

Hamajima1997 K. Hamajima, J. Fukushima, H. Bukawa, T. Kaneko, T. Tsuji, Y. Asakura, S. Sasaki, K. Q. Xin, and K. Okuda. Strong Augment Effect of IL-12 Expression Plasmid on the Induction of HIV-Specific Cytotoxic T Lymphocyte Activity by a Peptide Vaccine Candidate. Clin. Immunol. Immunopathol., 83:179-184, 1997. We previously reported that repeated inoculation of VC1, a macromolecular multicomponent peptide vaccine emulsified with Freund's adjuvant (VC1-F), induced high cytotoxic T lymphocyte (CTL) levels and a substantial level of multivalent antibodies which neutralized various human immunodeficiency virus type 1 (HIV-1) isolates. In the present study, we report that inoculation of VC1-F plus interleukin (IL)-12 expression plasmid can induce a higher antigen-specific CTL response compared to that with VC1-F alone. VC1-F plus IL-12 expression plasmid or VC1-F alone were inoculated to BALB/c mice twice at interval of 2 weeks. Two weeks after the second inoculation, spleen effector cells from these mice were examined. Stronger CTL responses against target cells were observed from the inoculation of VC1-F plus IL-12 plasmid than from that with VC-1F alone, but there was no difference in antibody induction. The inoculation of VC1 plus IL-12 plasmid also produced higher CTL activity than the inoculation of VC1 alone. These augmented CTL activities were not observed using target cells pulsed with non-HIV-specific peptides and different class I haplotype cells. These data demonstrate that co-inoculation of cell-mediated immune potent antigen and IL-12 plasmids can enhance the antigen-specific CTL response. This may be a potential approach for the induction of cellular immunization against HIV-1 and other diseases. PubMed ID: 9143379. Show all entries for this paper.


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