Search CTL/CD8+ T-Cell Epitope Database

Found 6 matching records:

Displaying record number 1088

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HXB2 Location	Nef(182-198) DNA(9340..9390)	Nef Epitope Map
Author Location	Nef(182-198 LAI)
Epitope	`EWRFDSRLAFHHVAREL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A2, A25)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

The C-terminal region of Nef (182-205) contains multiple CTL epitopes with 5 distinct HLA restrictions.

References

Hadida1995 F. Hadida, G. Haas, G. Zimmermann, A. Hosmalin, R. Spohn, A. Samri, G. Jung, P. Debre, and B. Autran. CTLs from Lymphoid Organs Recognize an Optimal HLA-A2 Restricted and HLA-B52 Restricted Nonapeptide and Several Epitopes in the C-Terminal Region of HIV-1 Nef. J. Immunol., 154:4174-4186, 1995. An in vitro limiting dilution analysis showed CTL recognition in the context of HLA B52 and A2.1, A2.2 and A2.4 in nanomolar concentrations. Molecular modeling suggests motifs important for peptide binding to the pocket of an HLA-A2.1 molecule. PubMed ID: 7535824. Show all entries for this paper.

Displaying record number 1087

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HXB2 Location	Nef(182-198) DNA(9340..9390)	Nef Epitope Map
Author Location	Nef(182-198 LAI)
Epitope	`EWRFDSRLAFHHVAREL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B35)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

The C-terminal region of Nef (182-205) contains multiple CTL epitopes with 5 distinct HLA restrictions.

References

Displaying record number 1091

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HXB2 Location	Nef(186-193) DNA(9352..9375)	Nef Epitope Map
Author Location	Nef(186-193 LAI)
Epitope	`DSRLAFHH`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B35)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

The C-terminal region of Nef (182-205) contains multiple CTL epitopes with 5 distinct HLA restrictions.

References

Displaying record number 1093

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HXB2 Location	Nef(188-196) DNA(9358..9384)	Nef Epitope Map
Author Location	Nef(188-196 LAI)
Epitope	`RLAFHHVAR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B52)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

The C-terminal region of Nef (182-205) contains multiple CTL epitopes with 5 distinct HLA restrictions.

References

Displaying record number 1095

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HXB2 Location	Nef(190-198) DNA(9364..9390)	Nef Epitope Map
Author Location	Nef(190-198 LAI)
Epitope	`AFHHVAREL`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 exposed seronegative
Experimental methods
Keywords	subtype comparisons, HIV exposed persistently seronegative (HEPS)

Notes

CTL recognition reported in the context of HLA-B52 and A2.1, A2.2 and A2.4.
A CTL response was found in exposed but uninfected prostitutes from Nairobi using previously-defined B clade epitopes that tended to be conserved in A and D clades -- such cross-reactivity could protect against both A and D and confer protection in Nairobi where both subtypes are circulating.
The A subtype consensus is ALKHRAYEL.
The D subtype consensus is AfEHKAREm.
Hunziker et al. Aids Res. Hum. Ret., 14(11): 921-4 (1998) suggests that HLA-A2 does not in fact present this epitope, and notes that it does not promote A2 assembly Connan1994 -- also see Brander et al. Aids Res. Hum. Ret., 14(11): 923-94 (1998)
Hunziker et al. Aids Res. Hum. Ret., 14(11): 921-4 (1998) maintains that HLA-A2 does not present this epitope contrary to an earlier report Hadida1995, (also see Brander et al. Aids Res. Hum. Ret., 14(11): 923-94 (1998))---despite the position of Hunziker et al., Rowland-Jones and colleagues are confident that this epitope in its A clade form is presented by HLA-A*0201 and A*0202, and it is one of the most common responses seen in both seropositive and exposed-uninfected donors from Nairobi (Rupert Kaul, pers. comm.)
Database note: First author "RowlandJones" is also found as "Rowland-Jones" on annotated papers in this database.

References

Connan1994 F. Connan, F. Hlavac, J. Hoebeke, J. G. Guillet, and J. Choppin. A Simple Assay for Detection of Peptides Promoting the Assembly of HLA Class I Molecules. Eur. J. Immunol., 24:777-780, 1994. Peptides from influenza and HIV-1 were tested for their ability to promote the assembly of HLA-A2 and HLA-B51 molecules in T2 cell lysates. HIV Pol 476-484 allowed significant assembly of HLA-A2, and is a target for CTL. Nef peptide 186-194 produced significant assembly of HLA-B51. A hydrophobic anchor residue (V, L, I) at position 9 could occupy pocket F, and a hydrophobic residue (V, L) at position 3 or 4 may anchor to hydrophobic pocket D of HLA-B51. Proline at position 2 increases HLA-B51 anchoring. PubMed ID: 8125145. Show all entries for this paper.

RowlandJones1998b S. Rowland-Jones, T. Dong, P. Krausa, J. Sutton, H. Newell, K. Ariyoshi, F. Gotch, S. Sabally, T. Corrah, J. Kimani, K. MacDonald, F. Plummer, J. Ndinya-Achola, H. Whittle, and A. McMichael. The Role of Cytotoxic T Cells in HIV Infection. Dev. Biol. Stand., 92:209-214, 1998. In this paper CTL response to previously defined conserved epitopes was found in exposed but uninfected prostitutes in Nairobi. Subtypes A and D are circulating in this regions, and the reactive epitopes tended to be conserved. Similarly previous studies in the Gambia showed that exposed but uninfected prostitutes tended to have B35 presented CTL epitopes conserved between HIV-1 and HIV-2. It was suggested that what was special about B35 is simply that it presents epitopes found both in HIV-1 and HIV-2. PubMed ID: 9554277. Show all entries for this paper.

Displaying record number 1096

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HXB2 Location	Nef(190-198) DNA(9364..9390)	Nef Epitope Map
Author Location	Nef(190-198 LAI)
Epitope	`AFHHVAREK`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A3)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

Naturally occurring L to K anchor substitution abrogates A2 binding, but permits HLA-A3 binding.