Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 55579

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HXB2 Location	Gag(131-138) p17(131)-p24(6) DNA(1180..1203)	Gag Epitope Map
Author Location	Gag(133-141 NL-432 or NL-M20A)
Epitope	NYPIVQNL	Epitope Alignment
Epitope Name	Gag133-8
Subtype	B
Species (MHC/HLA)	human(A*24:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*24:02
Country	Japan
Experimental methods	CTL suppression of replication, Chromium-release assay, HLA binding
Keywords

Notes

• To clarify mechanisms of escape mutation accumulation in the population, the Japanese Nef138-10 (RYPLTFGWCF) epitope was studied amongst hemophiliacs and others, to determine replication suppression abilities of both the wild type and 2F (RfPLTFGWCF) mutant virus. This mutant is conserved due to reduced CTL suppression of viral replication, also preventing viral reversion to WT upon transfer to a new host.
• Epitope Gag133-8, NYPIVQNL, was used as a comparison for positive cytolytic activity of epitope-specific HLA-A*2402 clones against target cells prepulsed with corresponding peptide. These clones partially suppressed NL-M20A viral replication.

References

Fujiwara2008 Mamoru Fujiwara, Junko Tanuma, Hirokazu Koizumi, Yuka Kawashima, Kazutaka Honda, Saori Mastuoka-Aizawa, Sachi Dohki, Shinichi Oka, and Masafumi Takiguchi. Different Abilities of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-Type Human Immunodeficiency Virus Type 1 in New Hosts. J. Virol., 82(1):138-147, Jan 2008. PubMed ID: 17959671. Show all entries for this paper.

Displaying record number 55582

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HXB2 Location	Gag(261-269) p24(129-137) DNA(1570..1596)	Gag Epitope Map
Author Location	Gag(263-272 NL-432 or NL-M20A)
Epitope	IYKRWIILG	Epitope Alignment
Epitope Name	Gag263-10
Subtype	B
Species (MHC/HLA)	human(A*24:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*24:02
Country	Japan
Experimental methods	CTL suppression of replication, Chromium-release assay, HLA binding
Keywords

Notes

• To clarify mechanisms of escape mutation accumulation in the population, the Japanese Nef138-10 (RYPLTFGWCF) epitope was studied amongst hemophiliacs and others, to determine replication suppression abilities of both the wild type and 2F (RfPLTFGWCF) mutant virus. This mutant is conserved due to reduced CTL suppression of viral replication, also preventing viral reversion to WT upon transfer to a new host.
• Epitope Gag263-10, IYKRWIILG, was used as a comparison for positive cytolytic activity of epitope-specific HLA-A*2402 clones against target cells prepulsed with corresponding peptide. These clones partially suppressed NL-M20A viral replication.

References

Fujiwara2008 Mamoru Fujiwara, Junko Tanuma, Hirokazu Koizumi, Yuka Kawashima, Kazutaka Honda, Saori Mastuoka-Aizawa, Sachi Dohki, Shinichi Oka, and Masafumi Takiguchi. Different Abilities of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-Type Human Immunodeficiency Virus Type 1 in New Hosts. J. Virol., 82(1):138-147, Jan 2008. PubMed ID: 17959671. Show all entries for this paper.

Displaying record number 55580

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HXB2 Location	Pol(797-804) Integrase(82-89) DNA(4473..4496)	Pol Epitope Map
Author Location	Pol(797-804 NL-432 or NL-M20A)
Epitope	GYIEAEVI	Epitope Alignment
Epitope Name	Pol797-8
Subtype	B
Species (MHC/HLA)	human(A*24:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*24:02
Country	Japan
Experimental methods	CTL suppression of replication, Chromium-release assay, HLA binding
Keywords

Notes

• To clarify mechanisms of escape mutation accumulation in the population, the Japanese Nef138-10 (RYPLTFGWCF) epitope was studied amongst hemophiliacs and others, to determine replication suppression abilities of both the wild type and 2F (RfPLTFGWCF) mutant virus. This mutant is conserved due to reduced CTL suppression of viral replication, also preventing viral reversion to WT upon transfer to a new host.
• Epitope Pol797-8, GYIEAEVI, was used as a comparison for positive cytolytic activity of epitope-specific HLA-A*2402 clones against target cells prepulsed with corresponding peptide. These clones partially suppressed NL-M20A viral replication.

References

Fujiwara2008 Mamoru Fujiwara, Junko Tanuma, Hirokazu Koizumi, Yuka Kawashima, Kazutaka Honda, Saori Mastuoka-Aizawa, Sachi Dohki, Shinichi Oka, and Masafumi Takiguchi. Different Abilities of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-Type Human Immunodeficiency Virus Type 1 in New Hosts. J. Virol., 82(1):138-147, Jan 2008. PubMed ID: 17959671. Show all entries for this paper.

Displaying record number 55577

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HXB2 Location	Nef(134-143) DNA(9196..9225)	Nef Epitope Map
Author Location	Nef(138-147 SF2)
Epitope	RYPLTFGWCF	Epitope Alignment
		Show epitope variants
Epitope Name	Nef138-10
Subtype	B
Species (MHC/HLA)	human(A*24:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*24:02
Country	Japan
Experimental methods	CTL suppression of replication, Chromium-release assay, HLA binding, Tetramer binding
Keywords	escape

Notes

• To clarify mechanisms of escape mutation accumulation in the population, the Japanese Nef138-10 (RYPLTFGWCF) epitope was studied amongst hemophiliacs and others, to determine replication suppression abilities of both the wild type and 2F (RfPLTFGWCF) mutant virus. This mutant is conserved due to reduced CTL suppression of viral replication, also preventing viral reversion to WT upon transfer to a new host.
• 2F mutant for strain SF-2, RfPLTFGWCF, is associated with HLA-A*2402, but has also accumulated in the HLA-A*2402- population. It was shown to be an escape mutation due to the inability of Nef138-10 clones to lyse or suppress replication of 2F10F mutant-infected cells.
• CTL clones specific for the 2F mutant are elicited when the 2F virus infects a new host. Such clones are able to recognize and suppress replication of NL-432-2F10F-infected cells. 2F epitope presentation however, was weaker than that of the WT epitope in one patient harboring both viruses.
• RYPLTFGWCF epitope mutations found were RfPLTFGWCF (25/41), RYPLTFGWCy (8/41), RYPLcFGWCF (3/41), RfPLcFGWCF (1/41), RfPLlFGWCF (1/41), RfPiTFGWCF (1/41), RYPLTFGWpF (1/41) and RlPLTFGWCF (1/41) in 41 HLA-A*2402+ Japanese patients. In 22 HLA-A*2402- Japanese patients, mutations found were RfPLTFGWCF (3/22), RYPLTFGWCy (2/22), RYPLcFGWCF (2/22) and RfPLTFGWsF (1/22).

References

Fujiwara2008 Mamoru Fujiwara, Junko Tanuma, Hirokazu Koizumi, Yuka Kawashima, Kazutaka Honda, Saori Mastuoka-Aizawa, Sachi Dohki, Shinichi Oka, and Masafumi Takiguchi. Different Abilities of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-Type Human Immunodeficiency Virus Type 1 in New Hosts. J. Virol., 82(1):138-147, Jan 2008. PubMed ID: 17959671. Show all entries for this paper.

Displaying record number 55578

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HXB2 Location	Nef(134-143) DNA(9196..9225)	Nef Epitope Map
Author Location	Nef(138-147 NL-432 or NL-M20A)
Epitope	RYPLTFGWCY	Epitope Alignment
		Show epitope variants
Epitope Name	Nef138-10
Subtype	B
Species (MHC/HLA)	human(A*24:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*24:02
Country	Japan
Experimental methods	CTL suppression of replication, Chromium-release assay, HLA binding, Other, Tetramer binding
Keywords	escape

Notes

• To clarify mechanisms of escape mutation accumulation in the population, the Japanese Nef138-10 (RYPLTFGWCF) epitope was studied amongst hemophiliacs and others, to determine replication suppression abilities of both the wild type and 2F (RfPLTFGWCF) mutant virus. This mutant is conserved due to reduced CTL suppression of viral replication, also preventing viral reversion to WT upon transfer to a new host.
• Strain NL-432 or NL-M20A epitope, RYPLTFGWCY, was used to confirm that A*2402-restricted Nef138-10 specific-CTLs have cytolytic activity and suppress viral replication within CD4+ infected cells. These CTLs also recognized the corresponding SF2-strain epitope RYPLTFGWCF.
• CTL clones recognizing 2F mutant, RfPLTFGWCY, for strains NL-432 and NL-M20A suppressed viral replication completely.

References

Fujiwara2008 Mamoru Fujiwara, Junko Tanuma, Hirokazu Koizumi, Yuka Kawashima, Kazutaka Honda, Saori Mastuoka-Aizawa, Sachi Dohki, Shinichi Oka, and Masafumi Takiguchi. Different Abilities of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-Type Human Immunodeficiency Virus Type 1 in New Hosts. J. Virol., 82(1):138-147, Jan 2008. PubMed ID: 17959671. Show all entries for this paper.