Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 52914

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HXB2 Location	Pol(311-319) RT(156-164) DNA(3015..3041)	Pol Epitope Map
Author Location
Epitope	`SPAIFQSSM`	Epitope Alignment
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	epitope processing, assay or method development, optimal epitope

Notes

96% of optimally defined epitopes have one of only nine amino acids serving as the C-terminal anchor position. Seven amino acids are never found in this position and four are only present in 4% of cases. CD8 T-cell response to an epitope is shown to be best detected when the epitope is situated at the C-terminal end of a longer peptide, and authors suggest that EliSpot reagents would be better designed if peptides ended on known C-terminal anchors.
SPAIFQSSM is suggested to be the optimal epitope instead of SPAIFQSSMT.

References

Draenert2004 Rika Draenert, Christian Brander, Xu G. Yu, Marcus Altfeld, Cori L. Verrill, Margeret E. Feeney, Bruce D. Walker, and Philip J. R. Goulder. Impact of Intrapeptide Epitope Location on CD8 T Cell Recognition: Implications for Design of Overlapping Peptide Panels. AIDS, 18(6):871-876, 9 Apr 2004. PubMed ID: 15060434. Show all entries for this paper.

Displaying record number 52915

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HXB2 Location	Nef(116-124) DNA(9142..9168)	Nef Epitope Map
Author Location
Epitope	`HTQGYFPDW`	Epitope Alignment
Species (MHC/HLA)	human(B57)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	epitope processing, assay or method development, optimal epitope

Notes

96% of optimally defined epitopes have one of only nine amino acids serving as the C-terminal anchor position. Seven amino acids are never found in this position and four are only present in 4% of cases. CD8 T-cell response to an epitope is shown to be best detected when the epitope is situated at the C-terminal end of a longer peptide, and authors suggest that EliSpot reagents would be better designed if peptides ended on known C-terminal anchors.
HTQGYFPDW is suggested to be the optimal epitope instead of HTQGYFPDWQ since Gln is not described as a C-terminal anchor residue in any of the other optimally defined epitopes. HTQGYFPDW was also found to be recognized at two times lower peptide concentrations than HTQGYFPDWQ.

References

Displaying record number 52916

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HXB2 Location	Nef(120-128) DNA(9154..9180)	Nef Epitope Map
Author Location
Epitope	`YFPDWQNYT`	Epitope Alignment
Species (MHC/HLA)	human(A29)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	epitope processing, assay or method development, optimal epitope

Notes

96% of optimally defined epitopes have one of only nine amino acids serving as the C-terminal anchor position. Seven amino acids are never found in this position and four are only present in 4% of cases. CD8 T-cell response to an epitope is shown to be best detected when the epitope is situated at the C-terminal end of a longer peptide, and authors suggest that EliSpot reagents would be better designed if peptides ended on known C-terminal anchors.
Instead of YFPDWQNYT, YFPDWQNY was found to be the optimal epitope in one patient (data not shown). For the other patient tested, data shown, YFPDWQNYT was the optimal epitope, restricted by A29.