Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 61035

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HXB2 Location	Gag(197-205) p24(65-73) DNA(1378..1404)	Gag Epitope Map
Author Location	Gag
Epitope	`AMQMLKDTI`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Cytokine production
Keywords	vaccine-induced immune responses, vaccine antigen design

Notes

A recombinant auxotroph for pantothenate expressing Gag or RT, M.bovis rBCGΔCD, induced HIV-C RT-specific and truncated-Env-specific CTL response when used to immunize mice. Immune boost was provided by rMVA which expressed a polyprotein of Gag, RT, Tat, Nef and truncated Env. Higher numbers of total CTL and those specific to subdominant Gag and RT epitopes were detected, as well as higher frequencies of response to subdominant epitopes.
Gag AMQMLKDTI, was a subdominant CTL epitope used to test for immune response. Immune responses to HIV-1 Gag, RT and Env can be achieved when a mixture of the rBCGΔpanCD vaccines is used.

References

Chapman2013 Rosamund Chapman, Helen Stutz, William Jacobs, Jr., Enid Shephard, and Anna-Lise Williamson. Priming with Recombinant Auxotrophic BCG Expressing HIV-1 Gag, RT and Gp120 and Boosting with Recombinant MVA Induces a Robust T Cell Response in Mice. PLoS One, 8(8):e71601, 20 Aug 2013. PubMed ID: 23977084. Show all entries for this paper.

Displaying record number 61036

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HXB2 Location	Pol(472-482) RT(317-327) DNA(3498..3530)	Pol Epitope Map
Author Location	RT
Epitope	`VYYDPSKDLIA`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Cytokine production
Keywords	vaccine-induced immune responses, vaccine antigen design

Notes

A recombinant auxotroph for pantothenate expressing Gag or RT, M.bovis rBCGΔCD, induced HIV-C RT-specific and truncated-Env-specific CTL response when used to immunize mice. Immune boost was provided by rMVA which expressed a polyprotein of Gag, RT, Tat, Nef and truncated Env. Higher numbers of total CTL and those specific to subdominant Gag and RT epitopes were detected, as well as higher frequencies of response to subdominant epitopes.
RT VYYDPSKDLIA, was a subdominant CTL epitope used to test for immune response. Immune responses to HIV-1 Gag, RT and Env can be achieved when a mixture of the rBCGΔpanCD vaccines is used.

References

Displaying record number 59453

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HXB2 Location	gp160(311-320) gp120(311-320) DNA(7155..7184)	gp160 Epitope Map
Author Location	Env
Epitope	`RGPGRAFVTI`	Epitope Alignment
Epitope Name	Peptide H
Subtype	C
Species (MHC/HLA)	mouse(H-2D^d)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Cytokine production
Keywords	vaccine-induced immune responses, vaccine antigen design

Notes

A recombinant auxotroph for pantothenate expressing Gag or RT, M.bovis rBCGΔCD, induced HIV-C RT-specific and truncated-Env-specific CTL response when used to immunize mice. Immune boost was provided by rMVA which expressed a polyprotein of Gag, RT, Tat, Nef and truncated Env. Higher numbers of total CTL and those specific to subdominant Gag and RT epitopes were detected, as well as higher frequencies of response to subdominant epitopes.
Env Peptide H, RGPGRAFVTI, was the dominant CTL epitope used to test for immune response. Immune responses to HIV-1 Gag, RT and Env can be achieved when a mixture of the rBCGΔpanCD vaccines is used.