Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 53622

Download this epitope record as JSON.

HXB2 Location	Gag(77-85) p17(77-85) DNA(1018..1044)	Gag Epitope Map
Author Location	p17(77-85 HXB2)
Epitope	`SLYNTVATL`	Epitope Alignment
Epitope Name	17A
Subtype	B
Species (MHC/HLA)	transgenic mouse(A2)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Cytokine production
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	DNA
Vaccine strain	multiple epitope immunogen
Vaccine component	p17/p24 Gag, Pol
Adjuvant	IL-12

Notes

Immunization of transgenic mice with a codon-optimized hGagp17p24-Polp51 DNA plasmid, consisting of clusters of highly conserved CTL epitopes presented by multiple MHC class I alleles, induced 2- to 5-fold higher CD8+ T-cell responses than the corresponding full-length proteins. The modified proteins had the ribosomal frameshift deleted, as well as the potentially immunosuppressive p15, and protease and integrase. This correlated with higher protection against challenge with Gag and Pol expressing recombinant vaccinia virus. Mice immunized with the hGagp17p24-Polp51 also showed an elevated level of type 1 cytokine production as well as an increased titer of p24- and RT-specific IgG2 antibody responses.
This was 1 of 4 A2 gag/pol epitopes tested. Transgenic mice immunized with the deleted construct induced more potent EliSpot reactions to this epitope than those immunized with full length Gag/Pol.

References

Bolesta2005a Elizabeth Bolesta, Jaroslaw Gzyl, Andrzej Wierzbicki, Dariusz Kmieciak, Aleksandra Kowalczyk, Yutaro Kaneko, Alagarsamy Srinivasan, and Danuta Kozbor. Clustered Epitopes within the Gag-Pol Fusion Protein DNA Vaccine Enhance Immune Responses and Protection against Challenge with Recombinant Vaccinia Viruses Expressing HIV-1 Gag and Pol Antigens. Virology, 332(2):467-479, 20 Feb 2005. PubMed ID: 15680412. Show all entries for this paper.

Displaying record number 53623

Download this epitope record as JSON.

HXB2 Location	Gag(151-159) p24(19-27) DNA(1240..1266)	Gag Epitope Map
Author Location	p24(19-27 HXB2)
Epitope	`TLNAWVKVI`	Epitope Alignment
Epitope Name	24D
Subtype	B
Species (MHC/HLA)	transgenic mouse(A2)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Cytokine production
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	DNA
Vaccine strain	multiple epitope immunogen
Vaccine component	p17/p24 Gag, Pol
Adjuvant	IL-12

Notes

Immunization of transgenic mice with a codon-optimized hGagp17p24-Polp51 DNA plasmid, consisting of clusters of highly conserved CTL epitopes presented by multiple MHC class I alleles, induced 2- to 5-fold higher CD8+ T-cell responses than the corresponding full-length proteins. The modified proteins had the ribosomal frameshift deleted, as well as the potentially immunosuppressive p15, and protease and integrase. This correlated with higher protection against challenge with Gag and Pol expressing recombinant vaccinia virus. Mice immunized with the hGagp17p24-Polp51 also showed an elevated level of type 1 cytokine production as well as an increased titer of p24- and RT-specific IgG2 antibody responses.
This was 1 of 4 A2 gag/pol epitopes tested. Transgenic mice immunized with the deleted construct induced more potent EliSpot reactions to this epitope than those immunized with full length Gag/Pol.

References

Displaying record number 53624

Download this epitope record as JSON.

HXB2 Location	Pol(334-342) RT(179-187) DNA(3084..3110)	Pol Epitope Map
Author Location	RT(179-187 HXB2)
Epitope	`VIYQYMDDL`	Epitope Alignment
Epitope Name	51F
Subtype	B
Species (MHC/HLA)	transgenic mouse(A2)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Cytokine production
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	DNA
Vaccine strain	multiple epitope immunogen
Vaccine component	p17/p24 Gag, Pol
Adjuvant	IL-12

Notes

Immunization of transgenic mice with a codon-optimized hGagp17p24-Polp51 DNA plasmid, consisting of clusters of highly conserved CTL epitopes presented by multiple MHC class I alleles, induced 2- to 5-fold higher CD8+ T-cell responses than the corresponding full-length proteins. The modified proteins had the ribosomal frameshift deleted, as well as the potentially immunosuppressive p15, and protease and integrase. This correlated with higher protection against challenge with Gag and Pol expressing recombinant vaccinia virus. Mice immunized with the hGagp17p24-Polp51 also showed an elevated level of type 1 cytokine production as well as an increased titer of p24- and RT-specific IgG2 antibody responses.
This was 1 of 4 A2 gag/pol epitopes tested. Transgenic mice immunized with the deleted construct induced more potent EliSpot reactions to this epitope than those immunized with full length Gag/Pol.

References

Displaying record number 53625

Download this epitope record as JSON.

HXB2 Location	Pol(640-649) RT(485-494) DNA(4002..4031)	Pol Epitope Map
Author Location	RT(485-495 HXB2)
Epitope	`ALQDSGSEVN`	Epitope Alignment
Epitope Name	51H
Subtype	B
Species (MHC/HLA)	transgenic mouse(A2)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Cytokine production
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	DNA
Vaccine strain	multiple epitope immunogen
Vaccine component	p17/p24 Gag, Pol
Adjuvant	IL-12

Notes

Immunization of transgenic mice with a codon-optimized hGagp17p24-Polp51 DNA plasmid, consisting of clusters of highly conserved CTL epitopes presented by multiple MHC class I alleles, induced 2- to 5-fold higher CD8+ T-cell responses than the corresponding full-length proteins. The modified proteins had the ribosomal frameshift deleted, as well as the potentially immunosuppressive p15, and protease and integrase. This correlated with higher protection against challenge with Gag and Pol expressing recombinant vaccinia virus. Mice immunized with the hGagp17p24-Polp51 also showed an elevated level of type 1 cytokine production as well as an increased titer of p24- and RT-specific IgG2 antibody responses.
This was 1 of 4 A2 gag/pol epitopes tested. Transgenic mice immunized with the deleted construct induced more potent EliSpot reactions to this epitope than those immunized with full length Gag/Pol.

References

Displaying record number 53626

Download this epitope record as JSON.

HXB2 Location	gp160(121-129) gp120(121-129) DNA(6585..6611)	gp160 Epitope Map
Author Location	Env(121-129 HXB2)
Epitope	`KLTPLCVTL`	Epitope Alignment
Epitope Name	D1
Subtype	B
Species (MHC/HLA)	transgenic mouse(A2)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Cytokine production
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	DNA
Vaccine strain	multiple epitope immunogen
Vaccine component	p17/p24 Gag, Pol
Adjuvant	IL-12

Notes

Immunization of transgenic mice with a codon-optimized hGagp17p24-Polp51 DNA plasmid, consisting of clusters of highly conserved CTL epitopes presented by multiple MHC class I alleles, induced 2- to 5-fold higher CD8+ T-cell responses than the corresponding full-length proteins. The modified proteins had the ribosomal frameshift deleted, as well as the potentially immunosuppressive p15, and protease and integrase. This correlated with higher protection against challenge with Gag and Pol expressing recombinant vaccinia virus. Mice immunized with the hGagp17p24-Polp51 also showed an elevated level of type 1 cytokine production as well as an increased titer of p24- and RT-specific IgG2 antibody responses.
Four A2 gag/pol epitopes were tested, and this Env A2 epitope was used as a negative control.