Search CTL/CD8+ T-Cell Epitope Database

Found 13 matching records:

Displaying record number 62493

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HXB2 Location	Gag(18-26) p17(18-26) DNA(841..867)	Gag Epitope Map
Author Location	Gag(18-26)
Epitope	`KIRLRPGGK`	Epitope Alignment
Epitope Name	KK9
Species (MHC/HLA)	human(A*03)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
A KIRLRPGGK-specific CTL clone cross-recognized an additional 1 of 8 A*03-restricted epitopes (RILSTYLGR).

References

Balamurugan2018 Arumugam Balamurugan, Hwee L. Ng, and Otto O. Yang. Cross-Reactivity against Multiple HIV-1 Epitopes Is Characteristic of HIV-1-Specific Cytotoxic T Lymphocyte Clones. J. Virol., 92(16), 15 Aug 2018. PubMed ID: 29899094. Show all entries for this paper.

Displaying record number 62494

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HXB2 Location	Gag(20-29) p17(20-29) DNA(847..876)	Gag Epitope Map
Author Location	Gag(20-29)
Epitope	`RLRPGGKKKY`	Epitope Alignment
Epitope Name	RY10
Species (MHC/HLA)	human(B*15)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
A RLRPGGKKKY-specific CTL clone cross-recognized 3 of 13 B*15-restricted epitopes (GLNKIVRMY, RMRRAEPAA and TQGYFPDWQNY).

References

Displaying record number 62487

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HXB2 Location	Gag(77-85) p17(77-85) DNA(1018..1044)	Gag Epitope Map
Author Location	Gag(77-85)
Epitope	`SLYNTVATL`	Epitope Alignment
Epitope Name	SL9
Species (MHC/HLA)	human(A*02)
Immunogen	in vitro stimulation or selection, engineered
Experimental methods	CD8 T-cell Elispot - IFNγ, CTL suppression of replication, Chromium-release assay, Peptide titration
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells, neutralization by CTL

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
Seven SLYNTVATL-specific CTL clones, established from 4 subjects, each cross-recognized an additional 4 to 12 of 28 A*02-restricted epitopes but with substantially lower functional avidity.
The following epitopes were recognized by SLYNTVATL-specific CTL clones: TLNAWVKVV (7/7 clones), VLAEAMSQV (4/7), FLGKIWPSHK (4/7), LVGPTPVNI (4/7), ALVEICTEM (3/7), IYQYMDDLYV (4/7), ILKEPVHGV (7/7), QMHEDIISL (6/7), RLISCNTSV (5/7), LLNATAIAV (2/7), VLEWRFDSR (3/7), AFHHVAREL (4/7) and RILQQLLFI (2/7).
Despite these cross-reactivities, there was no viral suppression in 2/2 SLYNTVATL-specific CTL clones when co-cultured with a T-cell line infected with NL4-3 HIV modified to include a SL9 escape mutant.

References

Displaying record number 62497

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HXB2 Location	Gag(147-155) p24(15-23) DNA(1228..1254)	Gag Epitope Map
Author Location	Gag(147-155)
Epitope	`ISPRTLNAW`	Epitope Alignment
Epitope Name	IW9
Species (MHC/HLA)	human(B*57)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
Unlike most CTL clones in this study, five ISPRTLNAW-specific CTL clones, established from 3 subjects, failed to cross-recognize 12 B*57-restricted epitopes.

References

Displaying record number 62500

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HXB2 Location	Gag(162-172) p24(30-40) DNA(1273..1305)	Gag Epitope Map
Author Location	Gag(162-172)
Epitope	`KAFSPEVIPMF`	Epitope Alignment
Epitope Name	KF11
Species (MHC/HLA)	human(B*57)
Immunogen	in vitro stimulation or selection, engineered, virus
Experimental methods	CTL suppression of replication, Chromium-release assay, Peptide titration
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells, neutralization by CTL

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
Five KAFSPEVIPMF-specific CTL clones, established from a single subject each cross-recognized 3 to 4 of 12 B*57-restricted epitopes but with lower functional avidity.
The following epitopes were recognized by KAFSPEVIPMF-specific CTL clones: TSTLQEQIGW (5/5 clones), QASQEVKNW (4/5), HTQGYFPDWQ (5/5), and YFPDWQNYT (5/5).

References

Displaying record number 62498

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HXB2 Location	Gag(240-249) p24(108-117) DNA(1507..1536)	Gag Epitope Map
Author Location	Gag(240-249)
Epitope	`TSTLQEQIGW`	Epitope Alignment
Epitope Name	TW10
Species (MHC/HLA)	human(B*57)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay, Peptide titration
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
Three TSTLQEQIGW-specific CTL clones, established from 2 subjects, each cross-recognized 1 to 3 of 13 B*57-restricted epitopes but with lower functional avidity.
The following epitopes were recognized by TSTLQEQIGW-specific CTL clones: QASQEVKNW (1/3 clones), HTQGYFPDWQ (1/3) and YFPDWQNYT (3/3).

References

Displaying record number 62504

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HXB2 Location	Pol(357-365) RT(202-210) DNA(3153..3179)	Pol Epitope Map
Author Location	Pol(357-365)
Epitope	`IEELRQHLL`	Epitope Alignment
Epitope Name	IL9
Species (MHC/HLA)	human(B*40)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
Two IEELRQHLL-specific CTL clones, established from a single subject, each cross-recognized the same 1 of 12 B*40-restricted epitopes (KELYPLASL, Gag KL9 - as confirmed by author communication due to a misprint in Figure 2 as originally published. Corrected erratum has been published).

References

Displaying record number 62496

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HXB2 Location	Pol(464-472) RT(309-317) DNA(3474..3500)	Pol Epitope Map
Author Location	Pol(464-472)
Epitope	`ILKEPVHGV`	Epitope Alignment
Epitope Name	IV9
Species (MHC/HLA)	human(A*02)
Immunogen	in vitro stimulation or selection, engineered
Experimental methods	CTL suppression of replication, Chromium-release assay, Peptide titration
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells, neutralization by CTL

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
ILKEPVHGV-specific CTL clone 68A62 cross-recognized 2 of 28 A*02-restricted epitopes (TLNAWVKVV and LVGPTPVNI) but with substantially lower functional avidity and demonstrated viral suppression when co-cultured with a T-cell line infected with NL4-3 HIV.

References

Displaying record number 62502

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HXB2 Location	Pol(529-538) RT(374-383) DNA(3669..3698)	Pol Epitope Map
Author Location	Pol(529-538)
Epitope	`KIATESIVIW`	Epitope Alignment
Epitope Name	KW10
Species (MHC/HLA)	human(B*57)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
Unlike most CTL clones in this study, a KIATESIVIW-specific CTL clone failed to cross-recognize 12 B*57-restricted epitopes.

References

Displaying record number 62503

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HXB2 Location	Rev(14-23) DNA(6009..6038)	Rev Epitope Map
Author Location	Rev(14-23)
Epitope	`RTVRLIKLLY`	Epitope Alignment
Epitope Name	RY10
Species (MHC/HLA)	human(B*57)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
Unlike most CTL clones in this study, a RTVRLIKLLY-specific CTL clone failed to cross-recognize 12 B*57-restricted epitopes.

References

Displaying record number 62501

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HXB2 Location	Nef(116-125) DNA(9142..9171)	Nef Epitope Map
Author Location	Nef(116-125)
Epitope	`HTQGYFPDWQ`	Epitope Alignment
Epitope Name	HQ10
Species (MHC/HLA)	human(B*57)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay, Peptide titration
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
Six HTQGYFPDWQ-specific CTL clones, established from 2 subjects, each cross-recognized 0 to 4 of 12 B*57-restricted epitopes but with lower functional avidity.
The following epitopes were recognized by HTQGYFPDWQ-specific CTL clones: KAFSPEVIPMF (2/6 clones), TSTLQEQIGW (4/6 clones), QASQEVKNW (4/6), and YFPDWQNYT (4/6).

References

Displaying record number 62495

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HXB2 Location	Nef(117-127) DNA(9145..9177)	Nef Epitope Map
Author Location	Nef(117-127)
Epitope	`TQGYFPDWQNY`	Epitope Alignment
Epitope Name	TY11
Species (MHC/HLA)	human(B*15)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
Unlike most CTL clones in this study, two TQGYFPDWQNY-specific CTL clones, established from a single subject, failed to cross-recognize 13 B*15-restricted epitopes.

References

Displaying record number 62499

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HXB2 Location	Nef(120-128) DNA(9154..9180)	Nef Epitope Map
Author Location	Nef(120-128)
Epitope	`YFPDWQNYT`	Epitope Alignment
Epitope Name	YT9
Species (MHC/HLA)	human(B*57)
Immunogen	in vitro stimulation or selection
Experimental methods	Chromium-release assay
Keywords	assay or method development, variant cross-reactivity, characterizing CD8+ T cells

Notes

Low-avidity cross-reactivities against HLA-matched HIV epitopes were commonly found in HIV-specific CTL clones but did not contribute to viral suppression activity. In A*02- and B*57-restricted CTL clones, only primary epitope specificities were identified by a viral suppression assay but cross-reactivities were also identified by standard chromium release and CTL IFNγ EliSpot assays, which often use supraphysiological peptide concentrations.
Two YFPDWQNYT-specific CTL clones, established from a single subject, each cross-recognized the same 3 of 12 B*57-restricted epitopes (TSTLQEQIGW, QASQEVKNW, and HTQGYFPDWQ).