Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 52577

HXB2 Location	Pol(464-472) RT(309-317) DNA(3474..3500)	Pol Epitope Map
Author Location	Pol(476-484)
Epitope	ILKEPVHGV	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*0201)
Immunogen	in vitro stimulation or selection
Experimental methods
Keywords	epitope processing, dendritic cells

Notes

• This study demonstrates that lipopeptides carrying epitopes can be taken up by human dendritic cells, processed using different pathways, and recognized by epitope-specific CD8+ T-cells originally derived from HIV+ individuals. The RT ILKEPVHGV peptide was embedded in a longer peptide fragment in the lipopeptide, and was internalized by endocytosis and processed in the cytosol by proteasomal cleavage by following an endosome-to-cytosol pathway for processing and presentation. Administration of epoxomycin, a proteasome inhibitor, completely abrogated epitope presentation to a CD8+ T-cell line, while monensin, an inhibitor of acid-dependent endosomal enzyme activity did not.
• In contrast to the RT epitope, dendritic cell presentation of the Nef epitope QVPLRPMTYK embedded in a longer peptide in a lipopeptide was not inhibited by epoxomycin, but was inhibited by monensin, indicative of endocytolitic epitope processing.

References

Andrieu2003 Muriel Andrieu, Jean-François Desoutter, Estelle Loing, Jésintha Gaston, Daniel Hanau, Jean-Gérard Guillet, and Anne Hosmalin. Two Human Immunodeficiency Virus Vaccinal Lipopeptides Follow Different Cross-Presentation Pathways in Human Dendritic Cells. J. Virol., 77(2):1564-1570, Jan 2003. PubMed ID: 12502869. Show all entries for this paper.

Displaying record number 52578

HXB2 Location	Nef(73-82) DNA(9013..9042)	Nef Epitope Map
Author Location	Nef(73-82)
Epitope	QVPLRPMTYK	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*0301)
Immunogen	in vitro stimulation or selection
Experimental methods
Keywords	epitope processing, dendritic cells

Notes

• This study demonstrates that lipopeptides carrying epitopes can be taken up by human dendritic cells, processed using different pathways, and recognized by epitope-specific CD8+ T-cells originally derived from HIV+ individuals. The RT ILKEPVHGV peptide was embedded in a longer peptide fragment in the lipopeptide, and was internalized by endocytosis and processed in the cytosol by proteasomal cleavage by following an endosome-to-cytosol pathway for processing and presentation. Administration of epoxomycin, a proteasome inhibitor, completely abrogated epitope presentation to a CD8+ T-cell line, while monensin, an inhibitor of acid-dependent endosomal enzyme activity did not.
• In contrast to the RT epitope, dendritic cell presentation of the Nef epitope QVPLRPMTYK embedded in a longer peptide in a lipopeptide was not inhibited by epoxomycin, but was inhibited by monensin, indicative of endocytolitic epitope processing.

References

Andrieu2003 Muriel Andrieu, Jean-François Desoutter, Estelle Loing, Jésintha Gaston, Daniel Hanau, Jean-Gérard Guillet, and Anne Hosmalin. Two Human Immunodeficiency Virus Vaccinal Lipopeptides Follow Different Cross-Presentation Pathways in Human Dendritic Cells. J. Virol., 77(2):1564-1570, Jan 2003. PubMed ID: 12502869. Show all entries for this paper.