Search CTL/CD8+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 57822

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HXB2 Location	Gag(19-27) p17(19-27) DNA(844..870)	Gag Epitope Map
Author Location	p17(19-27)
Epitope	IRLRPGGKK	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Country	Australia
Experimental methods	CD8 T-cell Elispot - IFNγ, Tetramer binding
Keywords	rate of progression, escape, TCR usage

Notes

• CTL responses were examined in 19 B*27-positive long-term non-progressors. CTL escape at R264 in the immunodominant KK10 epitope was a major determinant of viral load. Escape mutants at other B*27-restricted epitopes were uncommon. Coreceptor polymorphisms and breadth of TCR repertoire responding to KK10 were not different between patients with versus without escape at KK10.
• Following generation of escape mutants at KK10, responses to the subdominant epitope IRLRPGGKK were detected in 2/19 patients.
• 17/17 chronically infected B*27-positive patients had wild-type sequence for subdominant epitope IRLRPGGKK.

References

Ammaranond2011 Palanee Ammaranond, David J. van Bockel, Kathy Petoumenos, Marylin McMurchie, Robert Finlayson, Melanie G. Middleton, Miles P. Davenport, Vanessa Venturi, Kazuo Suzuki, Linda Gelgor, John M. Kaldor, David A. Cooper, and Anthony D. Kelleher. HIV Immune Escape at an Immunodominant Epitope in HLA-B*27-Positive Individuals Predicts Viral Load Outcome. J. Immunol., 186(1):479-488, 1 Jan 2011. PubMed ID: 21115730. Show all entries for this paper.

Displaying record number 57821

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HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location	Gag(263-272)
Epitope	KRWIILGLNK	Epitope Alignment
		Show epitope variants
Epitope Name	KK10
Subtype	B
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Country	Australia
Experimental methods	CD8 T-cell Elispot - IFNγ, Tetramer binding
Keywords	rate of progression, immunodominance, escape, TCR usage

Notes

• CTL responses were examined in 19 B*27-positive long-term non-progressors. CTL escape at R264 in the immunodominant KK10 epitope was a major determinant of viral load. Escape mutants at other B*27-restricted epitopes were uncommon. Coreceptor polymorphisms and breadth of TCR repertoire responding to KK10 were not different between patients with/without escape at KK10.
• 12/19 chronically infected B*27-positive patients had wild-type KK10 epitope KRWIILGLNK (n=4) or common L268M variant KRWIImGLNK (n=8), while 7/19 carried previously-documented escape mutants KgWIILGLNK (R264G, n=3), KkWIvLGLNK (R264K, n=1), KkWIImGLNK (R264K, n=2) or KqWIImGLNK (R264Q, n=1).
• Substantial IFN-γ responses were measured from PBMCs stimulated with WT or L268M variant at pre-escape time points. No responses were detected to escape variants R264G, R264Q, R264K at pre- or post-escape time points.
• 19/19 B*27-positive patients had substantial proportions of CD8+ T cells specific for immunodominant epitope KK10.

References

Ammaranond2011 Palanee Ammaranond, David J. van Bockel, Kathy Petoumenos, Marylin McMurchie, Robert Finlayson, Melanie G. Middleton, Miles P. Davenport, Vanessa Venturi, Kazuo Suzuki, Linda Gelgor, John M. Kaldor, David A. Cooper, and Anthony D. Kelleher. HIV Immune Escape at an Immunodominant Epitope in HLA-B*27-Positive Individuals Predicts Viral Load Outcome. J. Immunol., 186(1):479-488, 1 Jan 2011. PubMed ID: 21115730. Show all entries for this paper.

Displaying record number 57823

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HXB2 Location	gp160(786-795) gp41(275-284) DNA(8580..8609)	gp160 Epitope Map
Author Location	gp41(786-795)
Epitope	GRRGWEALKY	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Country	Australia
Experimental methods	CD8 T-cell Elispot - IFNγ, Tetramer binding
Keywords	rate of progression, escape, TCR usage

Notes

• CTL responses were examined in 19 B*27-positive long-term non-progressors. CTL escape at R264 in the immunodominant KK10 epitope was a major determinant of viral load. Escape mutants at other B*27-restricted epitopes were uncommon. Coreceptor polymorphisms and breadth of TCR repertoire responding to KK10 were not different between patients with versus without escape at KK10.
• 15/18 chronically infected B*27-positive patients had wild-type sequence for subdominant epitope GRRGWEALKY. 3/18 carried possible escape mutants GqRGWEALKY or GhRGWEALKY.

References

Ammaranond2011 Palanee Ammaranond, David J. van Bockel, Kathy Petoumenos, Marylin McMurchie, Robert Finlayson, Melanie G. Middleton, Miles P. Davenport, Vanessa Venturi, Kazuo Suzuki, Linda Gelgor, John M. Kaldor, David A. Cooper, and Anthony D. Kelleher. HIV Immune Escape at an Immunodominant Epitope in HLA-B*27-Positive Individuals Predicts Viral Load Outcome. J. Immunol., 186(1):479-488, 1 Jan 2011. PubMed ID: 21115730. Show all entries for this paper.

Displaying record number 57824

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HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114)
Epitope	RRQDILDLWI	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Country	Australia
Experimental methods	CD8 T-cell Elispot - IFNγ, Tetramer binding
Keywords	rate of progression, escape, TCR usage

Notes

• CTL responses were examined in 19 B*27-positive long-term non-progressors. CTL escape at R264 in the immunodominant KK10 epitope was a major determinant of viral load. Escape mutants at other B*27-restricted epitopes were uncommon. Coreceptor polymorphisms and breadth of TCR repertoire responding to KK10 were not different between patients with versus without escape at KK10.
• 14/16 chronically infected B*27-positive patients had wild-type sequence for subdominant epitope RRQDILDLWI. 2/16 carried possible escape mutants RgQDILDLWI or ReQDILDLWI.

References

Ammaranond2011 Palanee Ammaranond, David J. van Bockel, Kathy Petoumenos, Marylin McMurchie, Robert Finlayson, Melanie G. Middleton, Miles P. Davenport, Vanessa Venturi, Kazuo Suzuki, Linda Gelgor, John M. Kaldor, David A. Cooper, and Anthony D. Kelleher. HIV Immune Escape at an Immunodominant Epitope in HLA-B*27-Positive Individuals Predicts Viral Load Outcome. J. Immunol., 186(1):479-488, 1 Jan 2011. PubMed ID: 21115730. Show all entries for this paper.