Search CTL/CD8+ T-Cell Epitope Database

Found 19 matching records:

Displaying record number 1259

HXB2 Location	Gag(18-26) p17(18-26) DNA(841..867)	Gag Epitope Map
Author Location	p17(18-26 SF2)
Epitope	`KIRLRPGGK`	Epitope Alignment
Species (MHC/HLA)	human(A*0301)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
The reactive peptide p17 gag WEKIRLRPGGKKKYK contained two A*0301-restricted epitopes, KIRLRPGGK and RLRPGGKKK A*0301.

References

Altfeld2001c M. Altfeld, M. M. Addo, R. L. Eldridge, X. G. Yu, S. Thomas, A. Khatri, D. Strick, M. N. Phillips, G. B. Cohen, S. A. Islam, S. A. Kalams, C. Brander, P. J. Goulder, E. S. Rosenberg, B. D. Walker, and The HIV Study Collaboration.. Vpr is preferentially targeted by CTL during HIV-1 infection. J. Immunol., 167(5):2743-52, 1 Sep 2001. URL: http://www.jimmunol.org/cgi/content/full/167/5/2743. PubMed ID: 11509618. Show all entries for this paper.

Displaying record number 1260

HXB2 Location	Gag(20-28) p17(20-28) DNA(847..873)	Gag Epitope Map
Author Location	p17(20-28 SF2)
Epitope	`RLRPGGKKK`	Epitope Alignment
Species (MHC/HLA)	human(A*0301)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
The reactive peptide p17 gag WEKIRLRPGGKKKYK contained two A*0301-restricted epitopes, KIRLRPGGK and RLRPGGKKK A*0301.

References

Displaying record number 1261

HXB2 Location	Gag(77-85) p17(77-85) DNA(1018..1044)	Gag Epitope Map
Author Location	p17(77-85 SF2)
Epitope	`SLYNTVATL`	Epitope Alignment
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods
Keywords	acute/early infection

Notes

The relative contribution of CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
Individuals with long-term nonprogressive and treated chronic HIV-1 infection targeted Vpr more frequently than individuals with treated acute infection.
Vpr is a frequent target of HIV-1 specific CD8+ T-cells -- a response was detected in 45% of individuals tested and Vpr and p17 were the most preferentially targeted proteins per unit length by CD8+ T-cells.
The A2 epitopes Vpr AIIRLLQQL and p17 SLYNTVATL do not account for the dominance of Vpr and p17, the result holds even when HLA-A2+ individuals are excluded.

References

Displaying record number 1258

HXB2 Location	Gag(355-363) p24(223-231) DNA(1852..1878)	Gag Epitope Map
Author Location	p24(223-231 SF2)
Epitope	`GPGHKARVL`	Epitope Alignment
Epitope Name	GL9
Species (MHC/HLA)	human(B*0702)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
The response to GPGHKARVL was dominant.

References

Displaying record number 1256

HXB2 Location	Pol(308-322) RT(153-167) DNA(3006..3050)	Pol Epitope Map
Author Location	RT( SF2)
Epitope	`WKGSPAIFQSSMTKI`	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
RT peptides SQIYPGIKVRQLCKL and WKGSPAIFQSSMTKI were recognized.

References

Displaying record number 1257

HXB2 Location	Pol(423-437) RT(268-282) DNA(3351..3395)	Pol Epitope Map
Author Location	RT( SF2)
Epitope	`SQIYPGIKVRQLCKL`	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
RT peptides SQIYPGIKVRQLCKL and WKGSPAIFQSSMTKI were recognized.

References

Displaying record number 1267

HXB2 Location	Vif(17-26) DNA(5089..5118)	Vif Epitope Map
Author Location	Vif(17-26 SF2)
Epitope	`RIRTWKSLVK`	Epitope Alignment
Epitope Name	RK10
Species (MHC/HLA)	human(A*0301)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
10/29 (35%) individuals tested responded to Vif.
This epitope was recognized by 3/15 individuals expressing A*0301 allele.
HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
Overlapping Vif peptides QVDRMRIRTWKSLVK and RIRTWKSLVKHHMYI both reacted with T-cells from AC-06 and contained epitope RIRTWKSLVK.

References

Displaying record number 1268

HXB2 Location	Vif(31-39) DNA(5131..5157)	Vif Epitope Map
Author Location	Vif(31-39 SF2)
Epitope	`ISKKAKGWF`	Epitope Alignment
Species (MHC/HLA)	human(B*5701)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
10/29 (35%) individuals tested responded to Vif.
This epitope was recognized by 2/6 individuals expressing B*5701 allele.

References

Displaying record number 1269

HXB2 Location	Vif(48-57) DNA(5182..5211)	Vif Epitope Map
Author Location	Vif(48-57 SF2)
Epitope	`HPRVSSEVHI`	Epitope Alignment
Epitope Name	HI10
Species (MHC/HLA)	human(B*0702)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
10/29 (35%) individuals tested responded to Vif.
This epitope was recognized by 3/8 individuals expressing B*0702 allele.
HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
Overlapping Vif peptides HHYESTHPRVSSEVH and THPRVSSEVHIPLG both reacted with T-cells from AC-06 and contained epitope HPRVSSEVHI.

References

Displaying record number 1270

HXB2 Location	Vif(102-111) DNA(5344..5373)	Vif Epitope Map
Author Location	Vif(102-111 SF2)
Epitope	`LADQLIHLHY`	Epitope Alignment
Species (MHC/HLA)	human(B*1801)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
10/29 (35%) individuals tested responded to Vif.
This epitope was recognized by 2/5 individuals expressing B*1801 allele.

References

Displaying record number 1262

HXB2 Location	Vpr(12-20) DNA(5592..5618)	Vpr Epitope Map
Author Location	Vpr(12-20 SF2)
Epitope	`REPHNEWTL`	Epitope Alignment
Species (MHC/HLA)	human(B*4002)
Immunogen	HIV-1 infection
Experimental methods
Keywords	acute/early infection

Notes

CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
Individuals with long-term nonprogressive and treated chronic HIV-1 infection targeted Vpr more frequently than individuals with treated acute infection.
Only one B*4002+ individual was tested, and had a CTL response against REPHNEWTL.
Vpr is a frequent target of HIV-1 specific CD8+ T-cells -- a response was detected in 45% of individuals tested and Vpr and p17 were the most preferentially targeted proteins per unit length by CD8+ T-cells.

References

Displaying record number 1263

HXB2 Location	Vpr(30-38) DNA(5646..5672)	Vpr Epitope Map
Author Location	Vpr(29-38 SF2)
Epitope	`AVRHFPRIW`	Epitope Alignment
Species (MHC/HLA)	human(B*5701)
Immunogen	HIV-1 infection
Experimental methods
Keywords	acute/early infection

Notes

CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
This epitope was recognized by 4/6 individuals expressing B*5701 allele.
Individuals with long-term nonprogressive and treated chronic HIV-1 infection targeted Vpr more frequently than individuals with treated acute infection.
Vpr is a frequent target of HIV-1 specific CD8+ T-cells -- a response was detected in 45% of individuals tested and Vpr and p17 were the most preferentially targeted proteins per unit length by CD8+ T-cells.

References

Displaying record number 1265

HXB2 Location	Vpr(34-42) DNA(5658..5684)	Vpr Epitope Map
Author Location	Vpr(34-42 SF2)
Epitope	`FPRIWLHGL`	Epitope Alignment
Epitope Name	FL9
Species (MHC/HLA)	human(B*0702)
Immunogen	HIV-1 infection
Experimental methods
Keywords	acute/early infection

Notes

CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
This epitope was recognized by 2/2 individuals expressing B*8101 allele and 4/8 individuals expressing B*0702 allele.
Individuals with long-term nonprogressive and treated chronic HIV-1 infection targeted Vpr more frequently than individuals with treated acute infection.
Vpr is a frequent target of HIV-1 specific CD8+ T-cells -- a response was detected in 45% of individuals tested and Vpr and p17 were the most preferentially targeted proteins per unit length by CD8+ T-cells.
HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
FPRIWLHGL was the only epitope identified in Vpr for AC-06.

References

Displaying record number 1264

HXB2 Location	Vpr(34-42) DNA(5658..5684)	Vpr Epitope Map
Author Location	Vpr(34-42 SF2)
Epitope	`FPRIWLHGL`	Epitope Alignment
Epitope Name	FL9
Species (MHC/HLA)	human(B*8101)
Immunogen	HIV-1 infection
Experimental methods
Keywords	acute/early infection

Notes

CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
This epitope was recognized by 2/2 individuals expressing B*8101 allele and 4/8 individuals expressing B*0702 allele.
Individuals with long-term nonprogressive and treated chronic HIV-1 infection targeted Vpr more frequently than individuals with treated acute infection.
Vpr is a frequent target of HIV-1 specific CD8+ T-cells -- a response was detected in 45% of individuals tested and Vpr and p17 were the most preferentially targeted proteins per unit length by CD8+ T-cells.

References

Displaying record number 1266

HXB2 Location	Vpr(59-67) DNA(5733..5759)	Vpr Epitope Map
Author Location	Vpr(58-66 SF2)
Epitope	`AIIRILQQL`	Epitope Alignment
Epitope Name	AL9
Species (MHC/HLA)	human(A*0201)
Immunogen	HIV-1 infection
Experimental methods
Keywords	acute/early infection

Notes

CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
This epitope was recognized by 8/24 individuals expressing A*0201 allele.
Epitope is located within a highly conserved alpha helix in Vpr.
Individuals with long-term nonprogressive and treated chronic HIV-1 infection targeted Vpr more frequently than individuals with treated acute infection.
Vpr is a frequent target of HIV-1 specific CD8+ T-cells -- a response was detected in 45% of individuals tested and Vpr and p17 were the most preferentially targeted proteins per unit length by CD8+ T-cells.
The A2 epitopes Vpr AIIRLLQQL and p17 SLYNTVATL do not account for the dominance of Vpr and p17, the result holds even when HLA-A2+ individuals are excluded.

References

Displaying record number 1252

HXB2 Location	gp160(842-856) gp41(331-345) DNA(8748..8792)	gp160 Epitope Map
Author Location	gp41( SF2)
Epitope	`HIPRRIRQGLERALL`	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
The only Env peptide recognized was gp41 HIPRRIRQGLERALL.

References

Displaying record number 1255

HXB2 Location	Nef(62-81) DNA(8980..9039)	Nef Epitope Map
Author Location	Nef( SF2)
Epitope	`EEEEVGFPVTPQVPLRPMTY`	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
Nef peptides PQVPLRRMTYKAAVDLSHFL, KAAVDLSHFLKEKGGLEGLI and EEEEVGFPVTPQVPLRPMTY were recognized and the first two share KAAVDLSHFL (a Cw8 epitope), the first and last share PQVPLRPMTY.

References

Displaying record number 1253

HXB2 Location	Nef(72-91) DNA(9010..9069)	Nef Epitope Map
Author Location	Nef( SF2)
Epitope	`PQVPLRRMTYKAAVDLSHFL`	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
Nef peptides PQVPLRRMTYKAAVDLSHFL, KAAVDLSHFLKEKGGLEGLI and EEEEVGFPVTPQVPLRPMTY were recognized and the first two share KAAVDLSHFL (a Cw8 epitope), the first and last share PQVPLRPMTY.

References

Displaying record number 1254

HXB2 Location	Nef(82-101) DNA(9040..9099)	Nef Epitope Map
Author Location	Nef( SF2)
Epitope	`KAAVDLSHFLKEKGGLEGLI`	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

HIV+ individual AC-06 was tested for reactive overlapping peptides spanning all HIV-1 proteins in an ELISPOT and was found to react with 12 peptides from 7 proteins, suggesting that the breadth of CTL responses are underestimated if accessory proteins are not included in the study.
Nef peptides PQVPLRRMTYKAAVDLSHFL, KAAVDLSHFLKEKGGLEGLI and EEEEVGFPVTPQVPLRPMTY were recognized and the first two share KAAVDLSHFL (a Cw8 epitope), the first and last share PQVPLRPMTY.